Efecto del método de procesado sobre la microestructura y bioactividad de andamios eutectoides dentro del sistema 3CaOP2O5-2CaOSiO2
The increase in life expectancy in a population also leads to its aging, thus increasing the probability of suffering diseases related to bone tissue. Therefore, the development of biomaterials destined to bone tissue regeneration is necessary. In this thesis, it is proposed to obtain ceramic materi...
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| Tipo de recurso: | tesis doctoral |
| Fecha de publicación: | 2020 |
| País: | España |
| Institución: | Universidad Miguel Hernández de Elche |
| Repositorio: | REDIUMH. Depósito Digital de la UMH |
| OAI Identifier: | oai:dspace.umh.es:11000/25538 |
| Acceso en línea: | http://hdl.handle.net/11000/25538 |
| Access Level: | acceso abierto |
| Palabra clave: | materiales cerámicos prótesis tecnología de materiales CDU::5 - Ciencias puras y naturales::57 - Biología |
| Sumario: | The increase in life expectancy in a population also leads to its aging, thus increasing the probability of suffering diseases related to bone tissue. Therefore, the development of biomaterials destined to bone tissue regeneration is necessary. In this thesis, it is proposed to obtain ceramic materials, within the Ca2SiO4-Ca3(PO4)2 system, using the Polymer Sponge Replica m ethod (REP) by immersing a polyurethane (PU) sponge in a Sol-gel suspension (SG) and another one from Slurry (BR), followed by a thermal treatment in order to obtain ceramics with a porous 3D structure. Subsequently, the physical-chemical, mineralogical, macro and microstructural characterization of the previously obtained materials will be carried out, as well as their bioactivity evaluation through in vitro tests in simulated body fluid (SFA) to study their possible application in bone tissue regeneration. The characterization of the materials obtained by both methods has been carried out by different techniques, such as XRD, SEM, ATR-FTIR and Hg Porosimetry. The results indicate the scaffolds acquired by SG and BR had similarities at the macrostructure level. However, they present differences in terms of the phases present, microporosity and microstructure. The scaffolds have an open porosity (~95) whose pore size ranges from 300-600μm. However, significant differences have been found in relation to microporosity, being 12.83% and 48.9% in the BR and SG scaffolds, respectively, Regarding the mineralogical composition, the BR scaffolds are biphasic due to the presence of the C2S and Nurse´s A phases, while the SG scaffolds are multiphasic because they present several minority phases and C2S and TCP as majority ones. The results show a lamellar microstructure of alternating layers of C2S and Nurse´s A for BR scaffolds, whereas SG scaffolds present globular microstructure generated by an agglomeration of particles which have lost their identity and have grown cylindrically. The bioactivity evaluation of the 3D scaffolds was determined by changes in the scaffold´s microstructure due to the dissolution/precipitation processes that take place after immersion in SBF. The characterization was performed by SEM and ICP-OES. Both samples are bioactive, because they generated a Hydroxyapatite (HA) precipitate, mineral component of bone tissue, on its surface. However, SG scaffolds exhibited slower bioactivity (14 d) compared to BR (6 h), thus presenting different reaction kinetics. |
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