Synthesis, RNAi activity and nuclease-resistant properties of apolar carbohydrates siRNA conjugates

Oligoribonucleotide conjugates carrying apolar carbohydrates at the 5′-end and the corresponding siRNA duplexes have been prepared using phosphoramidite chemistry. All the carbohydrate-siRNA derivatives were compatible with RNA interference machinery if transfected with oligofectamine. In the absenc...

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Detalles Bibliográficos
Autores: Vengut Climent, Empar, Terrazas Martínez, Montserrat, Lucas, Ricardo, Arévalo Ruiz, Matilde, Eritja i Casadellà, Ramon, Morales, Juan Carlos
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2013
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/179776
Acceso en línea:https://hdl.handle.net/2445/179776
Access Level:acceso abierto
Palabra clave:RNA
Oligonucleòtids
Química orgànica
Oligonucleotides
Organic chemistry
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spelling Synthesis, RNAi activity and nuclease-resistant properties of apolar carbohydrates siRNA conjugatesVengut Climent, EmparTerrazas Martínez, MontserratLucas, RicardoArévalo Ruiz, MatildeEritja i Casadellà, RamonMorales, Juan CarlosRNAOligonucleòtidsQuímica orgànicaRNAOligonucleotidesOrganic chemistryOligoribonucleotide conjugates carrying apolar carbohydrates at the 5′-end and the corresponding siRNA duplexes have been prepared using phosphoramidite chemistry. All the carbohydrate-siRNA derivatives were compatible with RNA interference machinery if transfected with oligofectamine. In the absence of a transfection agent, some of them exerted certain reduction of gene expression. Double-tailed permethylated glucose conjugated to siRNA through a long spacer inhibited gene expression up to 26% compared to the scrambled duplex. Such modifications contribute positively to the stability of oligoribonucleotides against 5′-exonuclease degradation.Elsevier Ltd2021202120132021info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion4 p.application/pdfhttps://hdl.handle.net/2445/179776Articles publicats en revistes (Química Inorgànica i Orgànica)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/10.1016/j.bmcl.2013.05.065Bioorganic & Medicinal Chemistry Letters, 2013, vol. 23, num. 14, p. 4048-4051https://doi.org/10.1016/j.bmcl.2013.05.065(c) Elsevier Ltd, 2013info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1797762026-05-29T05:05:01Z
dc.title.none.fl_str_mv Synthesis, RNAi activity and nuclease-resistant properties of apolar carbohydrates siRNA conjugates
title Synthesis, RNAi activity and nuclease-resistant properties of apolar carbohydrates siRNA conjugates
spellingShingle Synthesis, RNAi activity and nuclease-resistant properties of apolar carbohydrates siRNA conjugates
Vengut Climent, Empar
RNA
Oligonucleòtids
Química orgànica
RNA
Oligonucleotides
Organic chemistry
title_short Synthesis, RNAi activity and nuclease-resistant properties of apolar carbohydrates siRNA conjugates
title_full Synthesis, RNAi activity and nuclease-resistant properties of apolar carbohydrates siRNA conjugates
title_fullStr Synthesis, RNAi activity and nuclease-resistant properties of apolar carbohydrates siRNA conjugates
title_full_unstemmed Synthesis, RNAi activity and nuclease-resistant properties of apolar carbohydrates siRNA conjugates
title_sort Synthesis, RNAi activity and nuclease-resistant properties of apolar carbohydrates siRNA conjugates
dc.creator.none.fl_str_mv Vengut Climent, Empar
Terrazas Martínez, Montserrat
Lucas, Ricardo
Arévalo Ruiz, Matilde
Eritja i Casadellà, Ramon
Morales, Juan Carlos
author Vengut Climent, Empar
author_facet Vengut Climent, Empar
Terrazas Martínez, Montserrat
Lucas, Ricardo
Arévalo Ruiz, Matilde
Eritja i Casadellà, Ramon
Morales, Juan Carlos
author_role author
author2 Terrazas Martínez, Montserrat
Lucas, Ricardo
Arévalo Ruiz, Matilde
Eritja i Casadellà, Ramon
Morales, Juan Carlos
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv RNA
Oligonucleòtids
Química orgànica
RNA
Oligonucleotides
Organic chemistry
topic RNA
Oligonucleòtids
Química orgànica
RNA
Oligonucleotides
Organic chemistry
description Oligoribonucleotide conjugates carrying apolar carbohydrates at the 5′-end and the corresponding siRNA duplexes have been prepared using phosphoramidite chemistry. All the carbohydrate-siRNA derivatives were compatible with RNA interference machinery if transfected with oligofectamine. In the absence of a transfection agent, some of them exerted certain reduction of gene expression. Double-tailed permethylated glucose conjugated to siRNA through a long spacer inhibited gene expression up to 26% compared to the scrambled duplex. Such modifications contribute positively to the stability of oligoribonucleotides against 5′-exonuclease degradation.
publishDate 2013
dc.date.none.fl_str_mv 2013
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/179776
url https://hdl.handle.net/2445/179776
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1016/j.bmcl.2013.05.065
Bioorganic & Medicinal Chemistry Letters, 2013, vol. 23, num. 14, p. 4048-4051
https://doi.org/10.1016/j.bmcl.2013.05.065
dc.rights.none.fl_str_mv (c) Elsevier Ltd, 2013
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Elsevier Ltd, 2013
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 4 p.
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ltd
publisher.none.fl_str_mv Elsevier Ltd
dc.source.none.fl_str_mv Articles publicats en revistes (Química Inorgànica i Orgànica)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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