Assessing Blood-Based Biomarkers to Define a Therapeutic Window for Natalizumab

Natalizumab is a monoclonal antibody that binds CD49d. Although it is one of the most effective treatments for Relapsing-Remitting Multiple Sclerosis (RRMS), a dosing regimen has not been optimized for safety and efficacy in individual patients. We aimed to identify biomarkers to monitor Natalizumab...

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Autores: Granell-Geli, Júlia|||0000-0002-3369-9765, Izquierdo, Cristina, Sellés-Rius, Ares|||0000-0002-9745-7749, Teniente Serra, Aina|||0000-0003-3440-804X, Presas Rodríguez, Sílvia|||0000-0001-8545-2476, Mansilla Lopez, Maria Jose|||0000-0002-5044-6313, Brieva Ruiz, Luis|||0000-0002-5871-6189, Sotoca Fernández, Javier|||0000-0003-3400-1434, Mañé-Martínez, María Alba, Moral, Ester, Bragado, Irene, Goelz, Susan, Martínez Cáceres, Eva María|||0000-0002-6762-8025, Ramo-Tello, Cristina|||0000-0001-8643-5053
Formato: artículo
Fecha de publicación:2021
País:España
Recursos:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:282871
Acesso em linha:https://ddd.uab.cat/record/282871
https://dx.doi.org/urn:doi:10.3390/jpm11121347
Access Level:acceso abierto
Palavra-chave:Biomarker
CD49d
Extended interval dose
Immunomonitoring
Multiple sclerosis
Natalizumab
Personalized dose
SVCAM-1
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spelling Assessing Blood-Based Biomarkers to Define a Therapeutic Window for NatalizumabGranell-Geli, Júlia|||0000-0002-3369-9765Izquierdo, CristinaSellés-Rius, Ares|||0000-0002-9745-7749Teniente Serra, Aina|||0000-0003-3440-804XPresas Rodríguez, Sílvia|||0000-0001-8545-2476Mansilla Lopez, Maria Jose|||0000-0002-5044-6313Brieva Ruiz, Luis|||0000-0002-5871-6189Sotoca Fernández, Javier|||0000-0003-3400-1434Mañé-Martínez, María AlbaMoral, EsterBragado, IreneGoelz, SusanMartínez Cáceres, Eva María|||0000-0002-6762-8025Ramo-Tello, Cristina|||0000-0001-8643-5053BiomarkerCD49dExtended interval doseImmunomonitoringMultiple sclerosisNatalizumabPersonalized doseSVCAM-1Natalizumab is a monoclonal antibody that binds CD49d. Although it is one of the most effective treatments for Relapsing-Remitting Multiple Sclerosis (RRMS), a dosing regimen has not been optimized for safety and efficacy in individual patients. We aimed to identify biomarkers to monitor Natalizumab treatment and to establish a personalized dose utilizing an ongoing longitudinal study in 29 RRMS patients under Natalizumab with standard interval dose (SD) of 300 mg/4 wks or extended interval dose (EID) of 300 mg/6 wks. Blood samples were analyzed by flow cytometry to determine CD49d saturation and expression in several T and B lymphocytes subpopulations. Each patient was analyzed at two different timepoints separated by 3 Natalizumab administrations. Natalizumab and sVCAM-1 levels in serum were also analyzed using ELISA. To determine the reproducibility of various markers, two different timepoints were compared and no significant differences were observed for CD49d expression nor for saturation; SD patients had higher saturation levels (~80%) than EID patients (~60%). A positive correlation exists between CD49d saturation and Natalizumab serum levels. CD49d expression and saturation are stable parameters that could be used as biomarkers in the immunomonitoring of Natalizumab treatment. Moreover, Natalizumab and sVCAM-1 serum levels could be used to optimize an individual's dosing schedule.Universitat Autònoma de Barcelona 22021-01-0120212021-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/282871https://dx.doi.org/urn:doi:10.3390/jpm11121347reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2828712026-06-06T12:50:31Z
dc.title.none.fl_str_mv Assessing Blood-Based Biomarkers to Define a Therapeutic Window for Natalizumab
title Assessing Blood-Based Biomarkers to Define a Therapeutic Window for Natalizumab
spellingShingle Assessing Blood-Based Biomarkers to Define a Therapeutic Window for Natalizumab
Granell-Geli, Júlia|||0000-0002-3369-9765
Biomarker
CD49d
Extended interval dose
Immunomonitoring
Multiple sclerosis
Natalizumab
Personalized dose
SVCAM-1
title_short Assessing Blood-Based Biomarkers to Define a Therapeutic Window for Natalizumab
title_full Assessing Blood-Based Biomarkers to Define a Therapeutic Window for Natalizumab
title_fullStr Assessing Blood-Based Biomarkers to Define a Therapeutic Window for Natalizumab
title_full_unstemmed Assessing Blood-Based Biomarkers to Define a Therapeutic Window for Natalizumab
title_sort Assessing Blood-Based Biomarkers to Define a Therapeutic Window for Natalizumab
dc.creator.none.fl_str_mv Granell-Geli, Júlia|||0000-0002-3369-9765
Izquierdo, Cristina
Sellés-Rius, Ares|||0000-0002-9745-7749
Teniente Serra, Aina|||0000-0003-3440-804X
Presas Rodríguez, Sílvia|||0000-0001-8545-2476
Mansilla Lopez, Maria Jose|||0000-0002-5044-6313
Brieva Ruiz, Luis|||0000-0002-5871-6189
Sotoca Fernández, Javier|||0000-0003-3400-1434
Mañé-Martínez, María Alba
Moral, Ester
Bragado, Irene
Goelz, Susan
Martínez Cáceres, Eva María|||0000-0002-6762-8025
Ramo-Tello, Cristina|||0000-0001-8643-5053
author Granell-Geli, Júlia|||0000-0002-3369-9765
author_facet Granell-Geli, Júlia|||0000-0002-3369-9765
Izquierdo, Cristina
Sellés-Rius, Ares|||0000-0002-9745-7749
Teniente Serra, Aina|||0000-0003-3440-804X
Presas Rodríguez, Sílvia|||0000-0001-8545-2476
Mansilla Lopez, Maria Jose|||0000-0002-5044-6313
Brieva Ruiz, Luis|||0000-0002-5871-6189
Sotoca Fernández, Javier|||0000-0003-3400-1434
Mañé-Martínez, María Alba
Moral, Ester
Bragado, Irene
Goelz, Susan
Martínez Cáceres, Eva María|||0000-0002-6762-8025
Ramo-Tello, Cristina|||0000-0001-8643-5053
author_role author
author2 Izquierdo, Cristina
Sellés-Rius, Ares|||0000-0002-9745-7749
Teniente Serra, Aina|||0000-0003-3440-804X
Presas Rodríguez, Sílvia|||0000-0001-8545-2476
Mansilla Lopez, Maria Jose|||0000-0002-5044-6313
Brieva Ruiz, Luis|||0000-0002-5871-6189
Sotoca Fernández, Javier|||0000-0003-3400-1434
Mañé-Martínez, María Alba
Moral, Ester
Bragado, Irene
Goelz, Susan
Martínez Cáceres, Eva María|||0000-0002-6762-8025
Ramo-Tello, Cristina|||0000-0001-8643-5053
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universitat Autònoma de Barcelona
dc.subject.none.fl_str_mv Biomarker
CD49d
Extended interval dose
Immunomonitoring
Multiple sclerosis
Natalizumab
Personalized dose
SVCAM-1
topic Biomarker
CD49d
Extended interval dose
Immunomonitoring
Multiple sclerosis
Natalizumab
Personalized dose
SVCAM-1
description Natalizumab is a monoclonal antibody that binds CD49d. Although it is one of the most effective treatments for Relapsing-Remitting Multiple Sclerosis (RRMS), a dosing regimen has not been optimized for safety and efficacy in individual patients. We aimed to identify biomarkers to monitor Natalizumab treatment and to establish a personalized dose utilizing an ongoing longitudinal study in 29 RRMS patients under Natalizumab with standard interval dose (SD) of 300 mg/4 wks or extended interval dose (EID) of 300 mg/6 wks. Blood samples were analyzed by flow cytometry to determine CD49d saturation and expression in several T and B lymphocytes subpopulations. Each patient was analyzed at two different timepoints separated by 3 Natalizumab administrations. Natalizumab and sVCAM-1 levels in serum were also analyzed using ELISA. To determine the reproducibility of various markers, two different timepoints were compared and no significant differences were observed for CD49d expression nor for saturation; SD patients had higher saturation levels (~80%) than EID patients (~60%). A positive correlation exists between CD49d saturation and Natalizumab serum levels. CD49d expression and saturation are stable parameters that could be used as biomarkers in the immunomonitoring of Natalizumab treatment. Moreover, Natalizumab and sVCAM-1 serum levels could be used to optimize an individual's dosing schedule.
publishDate 2021
dc.date.none.fl_str_mv 2
2021-01-01
2021
2021-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/282871
https://dx.doi.org/urn:doi:10.3390/jpm11121347
url https://ddd.uab.cat/record/282871
https://dx.doi.org/urn:doi:10.3390/jpm11121347
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
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collection Dipòsit Digital de Documents de la UAB
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