Use of portable devices and confocal Raman spectrometers at different wavelength to obtain the spectral information of the main organic components in tomatoes (Solanum Lycopersicum) fruits

Tomato (Solanum lycopersicum) fruit samples, in two ripening stages, ripe (red) and unripe (green), collected from a cultivar in the North of Spain (Barrika, Basque Country), were analyzed directly, without any sample pretreatment, with two different Raman instruments (portable spectrometer coupled...

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Detalles Bibliográficos
Autores: Trebolazabala, Josu, Maguregui Hernando, Maite, Morillas Loroño, Héctor, De Diego Rodríguez, Alberto, Madariaga Mota, Juan Manuel
Tipo de recurso: artículo
Fecha de publicación:2013
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/29904
Acceso en línea:http://hdl.handle.net/10810/29904
Access Level:acceso abierto
Palabra clave:Portable Raman spectroscopy, Confocal Raman spectroscopy, Tomato, Carotenoids, Cuticular waxes, Polyphenols
portable Raman spectroscopy
confocal Raman spectroscopy
tomato
carotenoids
cuticular waxes
polyphenols
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repository_id_str
spelling Use of portable devices and confocal Raman spectrometers at different wavelength to obtain the spectral information of the main organic components in tomatoes (Solanum Lycopersicum) fruitsTrebolazabala, JosuMaguregui Hernando, MaiteMorillas Loroño, HéctorDe Diego Rodríguez, AlbertoMadariaga Mota, Juan ManuelPortable Raman spectroscopy, Confocal Raman spectroscopy, Tomato, Carotenoids, Cuticular waxes, Polyphenolsportable Raman spectroscopyconfocal Raman spectroscopytomatocarotenoidscuticular waxespolyphenolsTomato (Solanum lycopersicum) fruit samples, in two ripening stages, ripe (red) and unripe (green), collected from a cultivar in the North of Spain (Barrika, Basque Country), were analyzed directly, without any sample pretreatment, with two different Raman instruments (portable spectrometer coupled to a micro-videocamera and a confocal Raman microscope), using two different laser excitation wavelengths (514 and 785 nm, only for the confocal microscope). The combined use of these laser excitation wavelengths allows obtaining, in a short period of time, the maximum spectral information about the main organic compounds present in this fruit. The major identified components of unripe tomatoes were cutin and cuticular waxes. On the other hand, the main components on ripe tomatoes were carotenes, polyphenoles and polysaccharides. Among the carotenes, it was possible to distinguish the presence of lycopene from b-carotene with the help of both excitation wavelengths, but specially using the 514 nm one, which revealed specific overtones and combination tones of this type of carotene.This work has been financially supported by Research Project S-PE11-UN128 of the Basque Country government. Technical and support provided by SGIker (UPV/EHU, MICINN, GV/EJ, ESF) is gratefully acknowledged.Elsevier201820182013info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/29904reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoIngléshttps://www.sciencedirect.com/science/article/pii/S1386142512012723info:eu-repo/semantics/openAccessoai:addi.ehu.eus:10810/299042026-06-18T09:23:17Z
dc.title.none.fl_str_mv Use of portable devices and confocal Raman spectrometers at different wavelength to obtain the spectral information of the main organic components in tomatoes (Solanum Lycopersicum) fruits
title Use of portable devices and confocal Raman spectrometers at different wavelength to obtain the spectral information of the main organic components in tomatoes (Solanum Lycopersicum) fruits
spellingShingle Use of portable devices and confocal Raman spectrometers at different wavelength to obtain the spectral information of the main organic components in tomatoes (Solanum Lycopersicum) fruits
Trebolazabala, Josu
Portable Raman spectroscopy, Confocal Raman spectroscopy, Tomato, Carotenoids, Cuticular waxes, Polyphenols
portable Raman spectroscopy
confocal Raman spectroscopy
tomato
carotenoids
cuticular waxes
polyphenols
title_short Use of portable devices and confocal Raman spectrometers at different wavelength to obtain the spectral information of the main organic components in tomatoes (Solanum Lycopersicum) fruits
title_full Use of portable devices and confocal Raman spectrometers at different wavelength to obtain the spectral information of the main organic components in tomatoes (Solanum Lycopersicum) fruits
title_fullStr Use of portable devices and confocal Raman spectrometers at different wavelength to obtain the spectral information of the main organic components in tomatoes (Solanum Lycopersicum) fruits
title_full_unstemmed Use of portable devices and confocal Raman spectrometers at different wavelength to obtain the spectral information of the main organic components in tomatoes (Solanum Lycopersicum) fruits
title_sort Use of portable devices and confocal Raman spectrometers at different wavelength to obtain the spectral information of the main organic components in tomatoes (Solanum Lycopersicum) fruits
dc.creator.none.fl_str_mv Trebolazabala, Josu
Maguregui Hernando, Maite
Morillas Loroño, Héctor
De Diego Rodríguez, Alberto
Madariaga Mota, Juan Manuel
author Trebolazabala, Josu
author_facet Trebolazabala, Josu
Maguregui Hernando, Maite
Morillas Loroño, Héctor
De Diego Rodríguez, Alberto
Madariaga Mota, Juan Manuel
author_role author
author2 Maguregui Hernando, Maite
Morillas Loroño, Héctor
De Diego Rodríguez, Alberto
Madariaga Mota, Juan Manuel
author2_role author
author
author
author
dc.subject.none.fl_str_mv Portable Raman spectroscopy, Confocal Raman spectroscopy, Tomato, Carotenoids, Cuticular waxes, Polyphenols
portable Raman spectroscopy
confocal Raman spectroscopy
tomato
carotenoids
cuticular waxes
polyphenols
topic Portable Raman spectroscopy, Confocal Raman spectroscopy, Tomato, Carotenoids, Cuticular waxes, Polyphenols
portable Raman spectroscopy
confocal Raman spectroscopy
tomato
carotenoids
cuticular waxes
polyphenols
description Tomato (Solanum lycopersicum) fruit samples, in two ripening stages, ripe (red) and unripe (green), collected from a cultivar in the North of Spain (Barrika, Basque Country), were analyzed directly, without any sample pretreatment, with two different Raman instruments (portable spectrometer coupled to a micro-videocamera and a confocal Raman microscope), using two different laser excitation wavelengths (514 and 785 nm, only for the confocal microscope). The combined use of these laser excitation wavelengths allows obtaining, in a short period of time, the maximum spectral information about the main organic compounds present in this fruit. The major identified components of unripe tomatoes were cutin and cuticular waxes. On the other hand, the main components on ripe tomatoes were carotenes, polyphenoles and polysaccharides. Among the carotenes, it was possible to distinguish the presence of lycopene from b-carotene with the help of both excitation wavelengths, but specially using the 514 nm one, which revealed specific overtones and combination tones of this type of carotene.
publishDate 2013
dc.date.none.fl_str_mv 2013
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/29904
url http://hdl.handle.net/10810/29904
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S1386142512012723
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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