Molecular interaction and cellular location of RecA and CheW proteins in Salmonella enterica during SOS response and their implication in swarming

In addition to its role in DNA damage repair and recombination, the RecA protein, through its interaction with CheW, is involved in swarming motility, a form of flagella-dependent movement across surfaces. In order to better understand how SOS response modulates swarming, in this work the location o...

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Autores: Irazoki Sánchez, Oihane, Aranda Rodríguez, Jesús|||0000-0002-1135-095X, Zimmermann, Timo, Campoy Sánchez, Susana|||0000-0003-1369-0197, Barbé García, Jordi|||0000-0003-4408-013X
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:254254
Acceso en línea:https://ddd.uab.cat/record/254254
https://dx.doi.org/urn:doi:10.3389/fmicb.2016.01560
Access Level:acceso abierto
Palabra clave:SOS response
Swarming
Chemoreceptor polar arrays
Chemosensory cluster assembly
RecA
CheW
3D-STED
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spelling Molecular interaction and cellular location of RecA and CheW proteins in Salmonella enterica during SOS response and their implication in swarmingIrazoki Sánchez, OihaneAranda Rodríguez, Jesús|||0000-0002-1135-095XZimmermann, TimoCampoy Sánchez, Susana|||0000-0003-1369-0197Barbé García, Jordi|||0000-0003-4408-013XSOS responseSwarmingChemoreceptor polar arraysChemosensory cluster assemblyRecACheW3D-STEDIn addition to its role in DNA damage repair and recombination, the RecA protein, through its interaction with CheW, is involved in swarming motility, a form of flagella-dependent movement across surfaces. In order to better understand how SOS response modulates swarming, in this work the location of RecA and CheW proteins within the swarming cells has been studied by using super-resolution microscopy. Further, and after in silico docking studies, the specific RecA and CheW regions associated with the RecA-CheW interaction have also been confirmed by site-directed mutagenesis and immunoprecipitation techniques. Our results point out that the CheW distribution changes, from the cell poles to foci distributed in a helical pattern along the cell axis when SOS response is activated or RecA protein is overexpressed. In this situation, the CheW presents the same subcellular location as that of RecA, pointing out that the previously described RecA storage structures may be modulators of swarming motility. Data reported herein not only confirmed that the RecA-CheW pair is essential for swarming motility but it is directly involved in the CheW distribution change associated to SOS response activation. A model explaining not only the mechanism by which DNA damage modulates swarming but also how both the lack and the excess of RecA protein impair this motility is proposed. 22016-01-0120162016-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/254254https://dx.doi.org/urn:doi:10.3389/fmicb.2016.01560reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 BIO2016-77011-RAgència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2014/SGR-572open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2542542026-06-06T12:50:31Z
dc.title.none.fl_str_mv Molecular interaction and cellular location of RecA and CheW proteins in Salmonella enterica during SOS response and their implication in swarming
title Molecular interaction and cellular location of RecA and CheW proteins in Salmonella enterica during SOS response and their implication in swarming
spellingShingle Molecular interaction and cellular location of RecA and CheW proteins in Salmonella enterica during SOS response and their implication in swarming
Irazoki Sánchez, Oihane
SOS response
Swarming
Chemoreceptor polar arrays
Chemosensory cluster assembly
RecA
CheW
3D-STED
title_short Molecular interaction and cellular location of RecA and CheW proteins in Salmonella enterica during SOS response and their implication in swarming
title_full Molecular interaction and cellular location of RecA and CheW proteins in Salmonella enterica during SOS response and their implication in swarming
title_fullStr Molecular interaction and cellular location of RecA and CheW proteins in Salmonella enterica during SOS response and their implication in swarming
title_full_unstemmed Molecular interaction and cellular location of RecA and CheW proteins in Salmonella enterica during SOS response and their implication in swarming
title_sort Molecular interaction and cellular location of RecA and CheW proteins in Salmonella enterica during SOS response and their implication in swarming
dc.creator.none.fl_str_mv Irazoki Sánchez, Oihane
Aranda Rodríguez, Jesús|||0000-0002-1135-095X
Zimmermann, Timo
Campoy Sánchez, Susana|||0000-0003-1369-0197
Barbé García, Jordi|||0000-0003-4408-013X
author Irazoki Sánchez, Oihane
author_facet Irazoki Sánchez, Oihane
Aranda Rodríguez, Jesús|||0000-0002-1135-095X
Zimmermann, Timo
Campoy Sánchez, Susana|||0000-0003-1369-0197
Barbé García, Jordi|||0000-0003-4408-013X
author_role author
author2 Aranda Rodríguez, Jesús|||0000-0002-1135-095X
Zimmermann, Timo
Campoy Sánchez, Susana|||0000-0003-1369-0197
Barbé García, Jordi|||0000-0003-4408-013X
author2_role author
author
author
author
dc.subject.none.fl_str_mv SOS response
Swarming
Chemoreceptor polar arrays
Chemosensory cluster assembly
RecA
CheW
3D-STED
topic SOS response
Swarming
Chemoreceptor polar arrays
Chemosensory cluster assembly
RecA
CheW
3D-STED
description In addition to its role in DNA damage repair and recombination, the RecA protein, through its interaction with CheW, is involved in swarming motility, a form of flagella-dependent movement across surfaces. In order to better understand how SOS response modulates swarming, in this work the location of RecA and CheW proteins within the swarming cells has been studied by using super-resolution microscopy. Further, and after in silico docking studies, the specific RecA and CheW regions associated with the RecA-CheW interaction have also been confirmed by site-directed mutagenesis and immunoprecipitation techniques. Our results point out that the CheW distribution changes, from the cell poles to foci distributed in a helical pattern along the cell axis when SOS response is activated or RecA protein is overexpressed. In this situation, the CheW presents the same subcellular location as that of RecA, pointing out that the previously described RecA storage structures may be modulators of swarming motility. Data reported herein not only confirmed that the RecA-CheW pair is essential for swarming motility but it is directly involved in the CheW distribution change associated to SOS response activation. A model explaining not only the mechanism by which DNA damage modulates swarming but also how both the lack and the excess of RecA protein impair this motility is proposed.
publishDate 2016
dc.date.none.fl_str_mv 2
2016-01-01
2016
2016-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/254254
https://dx.doi.org/urn:doi:10.3389/fmicb.2016.01560
url https://ddd.uab.cat/record/254254
https://dx.doi.org/urn:doi:10.3389/fmicb.2016.01560
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 BIO2016-77011-R
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2014/SGR-572
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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