Differential inhibition of the TGF-b signaling pathway in HCC cells using the small molecule inhibitor LY2157299 and the D10 monoclonal antibody against TGF-β receptor Type II

We investigated blocking the TGF-b signaling pathway in HCC using two small molecule inhibitors (LY2157299, LY2109761) and a neutralizing humanized antibody (D10) against TGF-bRII. LY2157299 and LY2109761 inhibited HCC cell migration on Laminin-5, Fibronectin, Vitronectin, Fibrinogen and Collagen-I...

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Autores: Dituri, Francesco, Mazzocca, Antonio, Peidrò, Fernando Juan, Papappicco, Patrizia, Fabregat Romero, Isabel, De Santis, Flavia, Paradiso, Angelo, Sabbà, Carlo, Giannelli, Gianluigi
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/97493
Acesso em linha:https://hdl.handle.net/2445/97493
Access Level:acceso abierto
Palavra-chave:Anticossos monoclonals
Fosforilació
Càncer de fetge
Factors de creixement
Monoclonal antibodies
Phosphorylation
Liver cancer
Growth factors
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spelling Differential inhibition of the TGF-b signaling pathway in HCC cells using the small molecule inhibitor LY2157299 and the D10 monoclonal antibody against TGF-β receptor Type IIDituri, FrancescoMazzocca, AntonioPeidrò, Fernando JuanPapappicco, PatriziaFabregat Romero, IsabelDe Santis, FlaviaParadiso, AngeloSabbà, CarloGiannelli, GianluigiAnticossos monoclonalsFosforilacióCàncer de fetgeFactors de creixementMonoclonal antibodiesPhosphorylationLiver cancerGrowth factorsWe investigated blocking the TGF-b signaling pathway in HCC using two small molecule inhibitors (LY2157299, LY2109761) and a neutralizing humanized antibody (D10) against TGF-bRII. LY2157299 and LY2109761 inhibited HCC cell migration on Laminin-5, Fibronectin, Vitronectin, Fibrinogen and Collagen-I and de novo phosphorylation of pSMAD2. LY2157299 inhibited HCC migration and cell growth independently of the expression levels of TGF-bRII. In contrast to LY2157299, D10 showed a reduction in pSMAD2 only after a short exposure. This study supports the use of LY2157299 in clinical trials, and presents new insights into TGF-b receptor cycling in cancer cells.Public Library of Science (PLoS)2016201620132016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion15 p.application/pdfhttps://hdl.handle.net/2445/97493Articles publicats en revistes (Ciències Fisiològiques)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0067109PLoS One, 2013, vol. 8, num. 6, p. e67109http://dx.doi.org/10.1371/journal.pone.0067109cc-by (c) Dituri, Francesco et al., 2013http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/974932026-05-29T05:05:01Z
dc.title.none.fl_str_mv Differential inhibition of the TGF-b signaling pathway in HCC cells using the small molecule inhibitor LY2157299 and the D10 monoclonal antibody against TGF-β receptor Type II
title Differential inhibition of the TGF-b signaling pathway in HCC cells using the small molecule inhibitor LY2157299 and the D10 monoclonal antibody against TGF-β receptor Type II
spellingShingle Differential inhibition of the TGF-b signaling pathway in HCC cells using the small molecule inhibitor LY2157299 and the D10 monoclonal antibody against TGF-β receptor Type II
Dituri, Francesco
Anticossos monoclonals
Fosforilació
Càncer de fetge
Factors de creixement
Monoclonal antibodies
Phosphorylation
Liver cancer
Growth factors
title_short Differential inhibition of the TGF-b signaling pathway in HCC cells using the small molecule inhibitor LY2157299 and the D10 monoclonal antibody against TGF-β receptor Type II
title_full Differential inhibition of the TGF-b signaling pathway in HCC cells using the small molecule inhibitor LY2157299 and the D10 monoclonal antibody against TGF-β receptor Type II
title_fullStr Differential inhibition of the TGF-b signaling pathway in HCC cells using the small molecule inhibitor LY2157299 and the D10 monoclonal antibody against TGF-β receptor Type II
title_full_unstemmed Differential inhibition of the TGF-b signaling pathway in HCC cells using the small molecule inhibitor LY2157299 and the D10 monoclonal antibody against TGF-β receptor Type II
title_sort Differential inhibition of the TGF-b signaling pathway in HCC cells using the small molecule inhibitor LY2157299 and the D10 monoclonal antibody against TGF-β receptor Type II
dc.creator.none.fl_str_mv Dituri, Francesco
Mazzocca, Antonio
Peidrò, Fernando Juan
Papappicco, Patrizia
Fabregat Romero, Isabel
De Santis, Flavia
Paradiso, Angelo
Sabbà, Carlo
Giannelli, Gianluigi
author Dituri, Francesco
author_facet Dituri, Francesco
Mazzocca, Antonio
Peidrò, Fernando Juan
Papappicco, Patrizia
Fabregat Romero, Isabel
De Santis, Flavia
Paradiso, Angelo
Sabbà, Carlo
Giannelli, Gianluigi
author_role author
author2 Mazzocca, Antonio
Peidrò, Fernando Juan
Papappicco, Patrizia
Fabregat Romero, Isabel
De Santis, Flavia
Paradiso, Angelo
Sabbà, Carlo
Giannelli, Gianluigi
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Anticossos monoclonals
Fosforilació
Càncer de fetge
Factors de creixement
Monoclonal antibodies
Phosphorylation
Liver cancer
Growth factors
topic Anticossos monoclonals
Fosforilació
Càncer de fetge
Factors de creixement
Monoclonal antibodies
Phosphorylation
Liver cancer
Growth factors
description We investigated blocking the TGF-b signaling pathway in HCC using two small molecule inhibitors (LY2157299, LY2109761) and a neutralizing humanized antibody (D10) against TGF-bRII. LY2157299 and LY2109761 inhibited HCC cell migration on Laminin-5, Fibronectin, Vitronectin, Fibrinogen and Collagen-I and de novo phosphorylation of pSMAD2. LY2157299 inhibited HCC migration and cell growth independently of the expression levels of TGF-bRII. In contrast to LY2157299, D10 showed a reduction in pSMAD2 only after a short exposure. This study supports the use of LY2157299 in clinical trials, and presents new insights into TGF-b receptor cycling in cancer cells.
publishDate 2013
dc.date.none.fl_str_mv 2013
2016
2016
2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/97493
url https://hdl.handle.net/2445/97493
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0067109
PLoS One, 2013, vol. 8, num. 6, p. e67109
http://dx.doi.org/10.1371/journal.pone.0067109
dc.rights.none.fl_str_mv cc-by (c) Dituri, Francesco et al., 2013
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Dituri, Francesco et al., 2013
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 15 p.
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLoS)
publisher.none.fl_str_mv Public Library of Science (PLoS)
dc.source.none.fl_str_mv Articles publicats en revistes (Ciències Fisiològiques)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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