Rescue of Aspergillus nidulans severely debilitating null mutations in ESCRT-0, I, II and III genes by inactivation of a salt-tolerance pathway allows examination of ESCRT gene roles in pH signalling

13 páginas, 11 figuras -- PAGS nros. 4064-4076

Detalles Bibliográficos
Autores: Calcagno-Pizarelli, Ana María, Hervás-Aguilar, América, Galindo, Antonio, Abenza, Juan F., Peñalva, Miguel Ángel, Arst, Herbert Nathan Jr.
Tipo de recurso: artículo
Fecha de publicación:2011
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/50458
Acceso en línea:http://hdl.handle.net/10261/50458
Access Level:acceso abierto
Palabra clave:PacC processing
Multivesicular body pathway
Endosomes
Arrestin
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repository_id_str
spelling Rescue of Aspergillus nidulans severely debilitating null mutations in ESCRT-0, I, II and III genes by inactivation of a salt-tolerance pathway allows examination of ESCRT gene roles in pH signallingCalcagno-Pizarelli, Ana MaríaHervás-Aguilar, AméricaGalindo, AntonioAbenza, Juan F.Peñalva, Miguel ÁngelArst, Herbert Nathan Jr.PacC processingMultivesicular body pathwayEndosomesArrestin13 páginas, 11 figuras -- PAGS nros. 4064-4076The Aspergillus pal pathway hijacks ESCRT proteins into ambient pH signalling complexes. We show that components of ESCRT-0, ESCRT-I, ESCRT-II and ESCRT-III are nearly essential for growth, precluding assessment of null mutants for pH signalling or trafficking. This severely debilitating effect is rescued by loss-of-function mutations in two cation tolerance genes, one of which, sltA, encodes a transcription factor whose inactivation promotes hypervacuolation. Exploiting a conditional expression sltA allele, we demonstrate that deletion of vps27 (ESCRT-0), vps23 (ESCRT-I), vps36 (ESCRT-II), or vps20 or vps32 (both ESCRT-III) leads to numerous small vacuoles, a phenotype also suppressed by SltA downregulation. This situation contrasts with normal vacuoles and vacuole-associated class E compartments seen in Saccharomyces cerevisiae ESCRT null mutants. Exploiting the suppressor phenotype of sltA− mutations, we establish that Vps23, Vps36, Vps20 and Vps32 are essential for pH signalling. Phosphatidylinositol 3-phosphate-recognising protein Vps27 (ESCRT-0) is not, consistent with normal pH signalling in rabB null mutants unable to recruit Vps34 kinase to early endosomes. In contrast to the lack of pH signalling in the absence of Vps20 or Vps32, detectable signalling occurs in the absence of ESCRT-III subunit Vps24. Our data support a model in which certain ESCRT proteins are recruited to the plasma membrane to mediate pH signallingThis work was supported by the Biotechnology and Biological Sciences Research Council [grant numbers BB/D521781/1 to H.N.A., BB/F01189X/1 to H.N.A. and Elaine Bignell]; the Wellcome Trust [grant numbers 067878, 084660/Z/08/Z to H.N.A. and Joan Tilburn]; the Spanish Government [grant number BIO2009-7281 to M.A.P.]; and Comunidad de Madrid [grant number SAL/0246/2006 to M.A.P.]. J.F.A. was a CSIC I3P fellow. Deposited in PMC for immediate releasePeer reviewedCompany of Biologists201220122011info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/50458reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1242/jcs.088344info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/504582026-05-22T06:33:51Z
dc.title.none.fl_str_mv Rescue of Aspergillus nidulans severely debilitating null mutations in ESCRT-0, I, II and III genes by inactivation of a salt-tolerance pathway allows examination of ESCRT gene roles in pH signalling
title Rescue of Aspergillus nidulans severely debilitating null mutations in ESCRT-0, I, II and III genes by inactivation of a salt-tolerance pathway allows examination of ESCRT gene roles in pH signalling
spellingShingle Rescue of Aspergillus nidulans severely debilitating null mutations in ESCRT-0, I, II and III genes by inactivation of a salt-tolerance pathway allows examination of ESCRT gene roles in pH signalling
Calcagno-Pizarelli, Ana María
PacC processing
Multivesicular body pathway
Endosomes
Arrestin
title_short Rescue of Aspergillus nidulans severely debilitating null mutations in ESCRT-0, I, II and III genes by inactivation of a salt-tolerance pathway allows examination of ESCRT gene roles in pH signalling
title_full Rescue of Aspergillus nidulans severely debilitating null mutations in ESCRT-0, I, II and III genes by inactivation of a salt-tolerance pathway allows examination of ESCRT gene roles in pH signalling
title_fullStr Rescue of Aspergillus nidulans severely debilitating null mutations in ESCRT-0, I, II and III genes by inactivation of a salt-tolerance pathway allows examination of ESCRT gene roles in pH signalling
title_full_unstemmed Rescue of Aspergillus nidulans severely debilitating null mutations in ESCRT-0, I, II and III genes by inactivation of a salt-tolerance pathway allows examination of ESCRT gene roles in pH signalling
title_sort Rescue of Aspergillus nidulans severely debilitating null mutations in ESCRT-0, I, II and III genes by inactivation of a salt-tolerance pathway allows examination of ESCRT gene roles in pH signalling
dc.creator.none.fl_str_mv Calcagno-Pizarelli, Ana María
Hervás-Aguilar, América
Galindo, Antonio
Abenza, Juan F.
Peñalva, Miguel Ángel
Arst, Herbert Nathan Jr.
author Calcagno-Pizarelli, Ana María
author_facet Calcagno-Pizarelli, Ana María
Hervás-Aguilar, América
Galindo, Antonio
Abenza, Juan F.
Peñalva, Miguel Ángel
Arst, Herbert Nathan Jr.
author_role author
author2 Hervás-Aguilar, América
Galindo, Antonio
Abenza, Juan F.
Peñalva, Miguel Ángel
Arst, Herbert Nathan Jr.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv PacC processing
Multivesicular body pathway
Endosomes
Arrestin
topic PacC processing
Multivesicular body pathway
Endosomes
Arrestin
description 13 páginas, 11 figuras -- PAGS nros. 4064-4076
publishDate 2011
dc.date.none.fl_str_mv 2011
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/50458
url http://hdl.handle.net/10261/50458
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1242/jcs.088344
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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