The incC sequence is required for R27 plasmid stability

IncHI plasmids account for multiple antimicrobial resistance in Salmonella and other enterobacterial genera. These plasmids are generally very stable in their bacterial hosts. R27 is the archetype of IncHI1 plasmids. A high percentage of the R27-encoded open reading frames (ORFs) (66.7%) do not show...

Descripción completa

Detalles Bibliográficos
Autores: Tassinari, Eleonora, Aznar Vicente, Sonia, Urcola, Imanol, Prieto Durán, Alejandro, Hüttener Queiroz, Mário, Juárez Giménez, Antonio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/112611
Acceso en línea:https://hdl.handle.net/2445/112611
Access Level:acceso abierto
Palabra clave:Seqüència d'aminoàcids
Plasmidis
Amino acid sequence
Plasmids
id ES_d5c60ecc7eaeea8e5158ae0d2c63ddbf
oai_identifier_str oai:diposit.ub.edu:2445/112611
network_acronym_str ES
network_name_str España
repository_id_str
spelling The incC sequence is required for R27 plasmid stabilityTassinari, EleonoraAznar Vicente, SoniaUrcola, ImanolPrieto Durán, AlejandroHüttener Queiroz, MárioJuárez Giménez, AntonioSeqüència d'aminoàcidsPlasmidisAmino acid sequencePlasmidsIncHI plasmids account for multiple antimicrobial resistance in Salmonella and other enterobacterial genera. These plasmids are generally very stable in their bacterial hosts. R27 is the archetype of IncHI1 plasmids. A high percentage of the R27-encoded open reading frames (ORFs) (66.7%) do not show similarity to any known ORFs. We performed a deletion analysis of all non-essential R27 DNA sequences to search for hitherto non-identified plasmid functions that might be required for plasmid stability. We report the identification of a short DNA sequence (incC) that is essential for R27 stability. That region contains several repeats (incC repeats), belongs to one of the three-plasmid replicons (R27 FIA-like) and is targeted by the R27 E protein. Deletion of the incC sequence drastically reduces R27 stability both in Escherichia coli and in Salmonella, the effect being more pronounced in this latter species. Interfering with incC-E protein interaction must lead to a reduced IncHI1 plasmid stability, and may represent a new approach to combat antimicrobial resistance.Frontiers Media2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/112611Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3389/fmicb.2016.00629Frontiers in Microbiology, 2016, vol. 7, num. 6, p. 629https://doi.org/10.3389/fmicb.2016.00629cc-by (c) Tassinari, Eleonora et al., 2016http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1126112026-05-27T06:46:51Z
dc.title.none.fl_str_mv The incC sequence is required for R27 plasmid stability
title The incC sequence is required for R27 plasmid stability
spellingShingle The incC sequence is required for R27 plasmid stability
Tassinari, Eleonora
Seqüència d'aminoàcids
Plasmidis
Amino acid sequence
Plasmids
title_short The incC sequence is required for R27 plasmid stability
title_full The incC sequence is required for R27 plasmid stability
title_fullStr The incC sequence is required for R27 plasmid stability
title_full_unstemmed The incC sequence is required for R27 plasmid stability
title_sort The incC sequence is required for R27 plasmid stability
dc.creator.none.fl_str_mv Tassinari, Eleonora
Aznar Vicente, Sonia
Urcola, Imanol
Prieto Durán, Alejandro
Hüttener Queiroz, Mário
Juárez Giménez, Antonio
author Tassinari, Eleonora
author_facet Tassinari, Eleonora
Aznar Vicente, Sonia
Urcola, Imanol
Prieto Durán, Alejandro
Hüttener Queiroz, Mário
Juárez Giménez, Antonio
author_role author
author2 Aznar Vicente, Sonia
Urcola, Imanol
Prieto Durán, Alejandro
Hüttener Queiroz, Mário
Juárez Giménez, Antonio
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Seqüència d'aminoàcids
Plasmidis
Amino acid sequence
Plasmids
topic Seqüència d'aminoàcids
Plasmidis
Amino acid sequence
Plasmids
description IncHI plasmids account for multiple antimicrobial resistance in Salmonella and other enterobacterial genera. These plasmids are generally very stable in their bacterial hosts. R27 is the archetype of IncHI1 plasmids. A high percentage of the R27-encoded open reading frames (ORFs) (66.7%) do not show similarity to any known ORFs. We performed a deletion analysis of all non-essential R27 DNA sequences to search for hitherto non-identified plasmid functions that might be required for plasmid stability. We report the identification of a short DNA sequence (incC) that is essential for R27 stability. That region contains several repeats (incC repeats), belongs to one of the three-plasmid replicons (R27 FIA-like) and is targeted by the R27 E protein. Deletion of the incC sequence drastically reduces R27 stability both in Escherichia coli and in Salmonella, the effect being more pronounced in this latter species. Interfering with incC-E protein interaction must lead to a reduced IncHI1 plasmid stability, and may represent a new approach to combat antimicrobial resistance.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/112611
url https://hdl.handle.net/2445/112611
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3389/fmicb.2016.00629
Frontiers in Microbiology, 2016, vol. 7, num. 6, p. 629
https://doi.org/10.3389/fmicb.2016.00629
dc.rights.none.fl_str_mv cc-by (c) Tassinari, Eleonora et al., 2016
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Tassinari, Eleonora et al., 2016
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869420755277578240
score 15,300719

Similares en LA Referencia