Hydrocolloids of egg white and gelatin as a platform for hydrogel-based tissue engineering

Innovative materials are needed to produce scaffolds for various tissue engineering and regenerative medicine (TERM) applications, including tissue models. Materials derived from natural sources that offer low production costs, easy availability, and high bioactivity are highly preferred. Chicken eg...

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Autores: Pele, Karinna Georgiana, Amaveda, Hippolyte, Mora, Mario, Marcuello, Carlos, Lostao, Anabel, Alamán-Díez, Pilar, Pérez-Huertas, Salvador, Pérez, María Ángeles, García-Aznar, José Manuel, García-Gareta, Elena
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Universidad de Zaragoza
Repositorio:Zaguán. Repositorio Digital de la Universidad de Zaragoza
OAI Identifier:oai:zaguan.unizar.es:126539
Acceso en línea:http://zaguan.unizar.es/record/126539
Access Level:acceso abierto
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spelling Hydrocolloids of egg white and gelatin as a platform for hydrogel-based tissue engineeringPele, Karinna GeorgianaAmaveda, HippolyteMora, MarioMarcuello, CarlosLostao, AnabelAlamán-Díez, PilarPérez-Huertas, SalvadorPérez, María ÁngelesGarcía-Aznar, José ManuelGarcía-Gareta, ElenaInnovative materials are needed to produce scaffolds for various tissue engineering and regenerative medicine (TERM) applications, including tissue models. Materials derived from natural sources that offer low production costs, easy availability, and high bioactivity are highly preferred. Chicken egg white (EW) is an overlooked protein-based material. Whilst its combination with the biopolymer gelatin has been investigated in the food technology industry, mixed hydrocolloids of EW and gelatin have not been reported in TERM. This paper investigates these hydrocolloids as a suitable platform for hydrogel-based tissue engineering, including 2D coating films, miniaturized 3D hydrogels in microfluidic devices, and 3D hydrogel scaffolds. Rheological assessment of the hydrocolloid solutions suggested that temperature and EW concentration can be used to fine-tune the viscosity of the ensuing gels. Fabricated thin 2D hydrocolloid films presented globular nano-topography and in vitro cell work showed that the mixed hydrocolloids had increased cell growth compared with EW films. Results showed that hydrocolloids of EW and gelatin can be used for creating a 3D hydrogel environment for cell studies inside microfluidic devices. Finally, 3D hydrogel scaffolds were fabricated by sequential temperature-dependent gelation followed by chemical cross-linking of the polymeric network of the hydrogel for added mechanical strength and stability. These 3D hydrogel scaffolds displayed pores, lamellae, globular nano-topography, tunable mechanical properties, high affinity for water, and cell proliferation and penetration properties. In conclusion, the large range of properties and characteristics of these materials provide a strong potential for a large variety of TERM applications, including cancer models, organoid growth, compatibility with bioprinting, or implantable devices.2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://zaguan.unizar.es/record/126539reponame:Zaguán. Repositorio Digital de la Universidad de Zaragozainstname:Universidad de ZaragozaInglésinfo:eu-repo/grantAgreement/ES/DGA/E09-23Rinfo:eu-repo/grantAgreement/ES/DGA/LMP176-21info:eu-repo/grantAgreement/EC/H2020/101018587This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 101018587-ICoMICSinfo:eu-repo/grantAgreement/ES/MICINN/PID2020-113819RB-I00info:eu-repo/grantAgreement/ES/MICINN/RYC2021-033490-Iinfo:eu-repo/grantAgreement/ES/MINECO-AEI-FEDER/PID2021-122409OB-C21info:eu-repo/semantics/openAccessoai:zaguan.unizar.es:1265392026-05-29T13:59:51Z
dc.title.none.fl_str_mv Hydrocolloids of egg white and gelatin as a platform for hydrogel-based tissue engineering
title Hydrocolloids of egg white and gelatin as a platform for hydrogel-based tissue engineering
spellingShingle Hydrocolloids of egg white and gelatin as a platform for hydrogel-based tissue engineering
Pele, Karinna Georgiana
title_short Hydrocolloids of egg white and gelatin as a platform for hydrogel-based tissue engineering
title_full Hydrocolloids of egg white and gelatin as a platform for hydrogel-based tissue engineering
title_fullStr Hydrocolloids of egg white and gelatin as a platform for hydrogel-based tissue engineering
title_full_unstemmed Hydrocolloids of egg white and gelatin as a platform for hydrogel-based tissue engineering
title_sort Hydrocolloids of egg white and gelatin as a platform for hydrogel-based tissue engineering
dc.creator.none.fl_str_mv Pele, Karinna Georgiana
Amaveda, Hippolyte
Mora, Mario
Marcuello, Carlos
Lostao, Anabel
Alamán-Díez, Pilar
Pérez-Huertas, Salvador
Pérez, María Ángeles
García-Aznar, José Manuel
García-Gareta, Elena
author Pele, Karinna Georgiana
author_facet Pele, Karinna Georgiana
Amaveda, Hippolyte
Mora, Mario
Marcuello, Carlos
Lostao, Anabel
Alamán-Díez, Pilar
Pérez-Huertas, Salvador
Pérez, María Ángeles
García-Aznar, José Manuel
García-Gareta, Elena
author_role author
author2 Amaveda, Hippolyte
Mora, Mario
Marcuello, Carlos
Lostao, Anabel
Alamán-Díez, Pilar
Pérez-Huertas, Salvador
Pérez, María Ángeles
García-Aznar, José Manuel
García-Gareta, Elena
author2_role author
author
author
author
author
author
author
author
author
description Innovative materials are needed to produce scaffolds for various tissue engineering and regenerative medicine (TERM) applications, including tissue models. Materials derived from natural sources that offer low production costs, easy availability, and high bioactivity are highly preferred. Chicken egg white (EW) is an overlooked protein-based material. Whilst its combination with the biopolymer gelatin has been investigated in the food technology industry, mixed hydrocolloids of EW and gelatin have not been reported in TERM. This paper investigates these hydrocolloids as a suitable platform for hydrogel-based tissue engineering, including 2D coating films, miniaturized 3D hydrogels in microfluidic devices, and 3D hydrogel scaffolds. Rheological assessment of the hydrocolloid solutions suggested that temperature and EW concentration can be used to fine-tune the viscosity of the ensuing gels. Fabricated thin 2D hydrocolloid films presented globular nano-topography and in vitro cell work showed that the mixed hydrocolloids had increased cell growth compared with EW films. Results showed that hydrocolloids of EW and gelatin can be used for creating a 3D hydrogel environment for cell studies inside microfluidic devices. Finally, 3D hydrogel scaffolds were fabricated by sequential temperature-dependent gelation followed by chemical cross-linking of the polymeric network of the hydrogel for added mechanical strength and stability. These 3D hydrogel scaffolds displayed pores, lamellae, globular nano-topography, tunable mechanical properties, high affinity for water, and cell proliferation and penetration properties. In conclusion, the large range of properties and characteristics of these materials provide a strong potential for a large variety of TERM applications, including cancer models, organoid growth, compatibility with bioprinting, or implantable devices.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://zaguan.unizar.es/record/126539
url http://zaguan.unizar.es/record/126539
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/ES/DGA/E09-23R
info:eu-repo/grantAgreement/ES/DGA/LMP176-21
info:eu-repo/grantAgreement/EC/H2020/101018587
This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 101018587-ICoMICS
info:eu-repo/grantAgreement/ES/MICINN/PID2020-113819RB-I00
info:eu-repo/grantAgreement/ES/MICINN/RYC2021-033490-I
info:eu-repo/grantAgreement/ES/MINECO-AEI-FEDER/PID2021-122409OB-C21
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv
publisher.none.fl_str_mv
dc.source.none.fl_str_mv reponame:Zaguán. Repositorio Digital de la Universidad de Zaragoza
instname:Universidad de Zaragoza
instname_str Universidad de Zaragoza
reponame_str Zaguán. Repositorio Digital de la Universidad de Zaragoza
collection Zaguán. Repositorio Digital de la Universidad de Zaragoza
repository.name.fl_str_mv
repository.mail.fl_str_mv
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