The Acetylglucosaminidase LytB of Streptococcus pneumoniae is Involved in the Structure and Formation of Biofilms

The N-acetylglucosaminidase LytB of Streptococcus pneumoniae is involved in nasopharyngeal colonization and is responsible for cell separation at the end of cell division; thus, ΔlytB mutants form long chains of cells. This paper reports the construction and properties of a defective pneumococcal mu...

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Detalhes bibliográficos
Autores: Domenech Lucas, Miriam, García, Ernesto
Formato: artículo
Fecha de publicación:2020
País:España
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:dnet:docta_______::c4fa7c272d863c7924926bab5cc378da
Acesso em linha:https://hdl.handle.net/20.500.14352/98133
Access Level:acceso abierto
Palavra-chave:579.25
Pneumococcus
Biofilm
LytB glucosaminidase
Choline-binding protein
Genética
Microbiología (Biología)
2409 Genética
2414 Microbiología
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oai_identifier_str oai:dnet:docta_______::c4fa7c272d863c7924926bab5cc378da
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repository_id_str
spelling The Acetylglucosaminidase LytB of Streptococcus pneumoniae is Involved in the Structure and Formation of BiofilmsDomenech Lucas, MiriamGarcía, Ernesto579.25PneumococcusBiofilmLytB glucosaminidaseCholine-binding proteinGenéticaMicrobiología (Biología)2409 Genética2414 MicrobiologíaThe N-acetylglucosaminidase LytB of Streptococcus pneumoniae is involved in nasopharyngeal colonization and is responsible for cell separation at the end of cell division; thus, ΔlytB mutants form long chains of cells. This paper reports the construction and properties of a defective pneumococcal mutant producing an inactive LytB protein (LytBE585A). It is shown that an enzymatically active LytB is required for in vitro biofilm formation, as lytB mutants (either ΔlytB or producing the inactive LytBE585A) are incapable of forming substantial biofilms, despite that extracellular DNA is present in the biofilm matrix. Adding small amounts (0.5 to 2.0 μg/ml) of exogenous LytB or some LytB constructs restored the biofilm-forming capacity of lytB mutants to wild-type levels. The LytBE585A mutant formed biofilm more rapidly than ΔlytB mutants in the presence of LytB. This suggests that the mutant protein acted in a structural role, likely through the formation of complexes with extracellular DNA. The chain-dispersing capacity of LytB allowed the separation of daughter cells, presumably facilitating the formation of microcolonies and, finally, of biofilms. A role for the possible involvement of LytB in the synthesis of the extracellular polysaccharide component of the biofilm matrix is also discussed.American Society for MicrobiologyUniversidad Complutense de Madrid20242024-02-0220202020-01-0120202020-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/98133reponame:Docta Complutenseinstname:Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dnet:docta_______::c4fa7c272d863c7924926bab5cc378da2025-08-02T12:44:52Z
dc.title.none.fl_str_mv The Acetylglucosaminidase LytB of Streptococcus pneumoniae is Involved in the Structure and Formation of Biofilms
title The Acetylglucosaminidase LytB of Streptococcus pneumoniae is Involved in the Structure and Formation of Biofilms
spellingShingle The Acetylglucosaminidase LytB of Streptococcus pneumoniae is Involved in the Structure and Formation of Biofilms
Domenech Lucas, Miriam
579.25
Pneumococcus
Biofilm
LytB glucosaminidase
Choline-binding protein
Genética
Microbiología (Biología)
2409 Genética
2414 Microbiología
title_short The Acetylglucosaminidase LytB of Streptococcus pneumoniae is Involved in the Structure and Formation of Biofilms
title_full The Acetylglucosaminidase LytB of Streptococcus pneumoniae is Involved in the Structure and Formation of Biofilms
title_fullStr The Acetylglucosaminidase LytB of Streptococcus pneumoniae is Involved in the Structure and Formation of Biofilms
title_full_unstemmed The Acetylglucosaminidase LytB of Streptococcus pneumoniae is Involved in the Structure and Formation of Biofilms
title_sort The Acetylglucosaminidase LytB of Streptococcus pneumoniae is Involved in the Structure and Formation of Biofilms
dc.creator.none.fl_str_mv Domenech Lucas, Miriam
García, Ernesto
author Domenech Lucas, Miriam
author_facet Domenech Lucas, Miriam
García, Ernesto
author_role author
author2 García, Ernesto
author2_role author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 579.25
Pneumococcus
Biofilm
LytB glucosaminidase
Choline-binding protein
Genética
Microbiología (Biología)
2409 Genética
2414 Microbiología
topic 579.25
Pneumococcus
Biofilm
LytB glucosaminidase
Choline-binding protein
Genética
Microbiología (Biología)
2409 Genética
2414 Microbiología
description The N-acetylglucosaminidase LytB of Streptococcus pneumoniae is involved in nasopharyngeal colonization and is responsible for cell separation at the end of cell division; thus, ΔlytB mutants form long chains of cells. This paper reports the construction and properties of a defective pneumococcal mutant producing an inactive LytB protein (LytBE585A). It is shown that an enzymatically active LytB is required for in vitro biofilm formation, as lytB mutants (either ΔlytB or producing the inactive LytBE585A) are incapable of forming substantial biofilms, despite that extracellular DNA is present in the biofilm matrix. Adding small amounts (0.5 to 2.0 μg/ml) of exogenous LytB or some LytB constructs restored the biofilm-forming capacity of lytB mutants to wild-type levels. The LytBE585A mutant formed biofilm more rapidly than ΔlytB mutants in the presence of LytB. This suggests that the mutant protein acted in a structural role, likely through the formation of complexes with extracellular DNA. The chain-dispersing capacity of LytB allowed the separation of daughter cells, presumably facilitating the formation of microcolonies and, finally, of biofilms. A role for the possible involvement of LytB in the synthesis of the extracellular polysaccharide component of the biofilm matrix is also discussed.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01
2020
2020-01-01
2024
2024-02-02
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/98133
url https://hdl.handle.net/20.500.14352/98133
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:
instname_str
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,638193

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