Development and Angiogenic Potential of Cell-Derived Microtissues Using Microcarrier-Template

Tissue engineering and regenerative medicine approaches use biomaterials in combination with cells to regenerate lost functions of tissues and organs to prevent organ transplantation. However, most of the current strategies fail in mimicking the tissue's extracellular matrix properties. In orde...

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Autores: Rubi Sans, Gerard, Cano Torres, Irene, Pérez Amodio, Soledad, Blanco Fernández, Bárbara, Mateos Timoneda, Miguel Ángel, Engel, Elisabeth
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/184454
Acceso en línea:https://hdl.handle.net/2445/184454
Access Level:acceso abierto
Palabra clave:Bioenginyeria
Medicina regenerativa
Angiogènesi
Bioengineering
Regenerative medicine
Neovascularization
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spelling Development and Angiogenic Potential of Cell-Derived Microtissues Using Microcarrier-TemplateRubi Sans, GerardCano Torres, IrenePérez Amodio, SoledadBlanco Fernández, BárbaraMateos Timoneda, Miguel ÁngelEngel, ElisabethBioenginyeriaMedicina regenerativaAngiogènesiBioengineeringRegenerative medicineNeovascularizationTissue engineering and regenerative medicine approaches use biomaterials in combination with cells to regenerate lost functions of tissues and organs to prevent organ transplantation. However, most of the current strategies fail in mimicking the tissue's extracellular matrix properties. In order to mimic native tissue conditions, we developed cell-derived matrix (CDM) microtissues (MT). Our methodology uses poly-lactic acid (PLA) and Cultispher(R) S microcarriers' (MCs') as scaffold templates, which are seeded with rat bone marrow mesenchymal stem cells (rBM-MSCs). The scaffold template allows cells to generate an extracellular matrix, which is then extracted for downstream use. The newly formed CDM provides cells with a complex physical (MT architecture) and biochemical (deposited ECM proteins) environment, also showing spontaneous angiogenic potential. Our results suggest that MTs generated from the combination of these two MCs (mixed MTs) are excellent candidates for tissue vascularization. Overall, this study provides a methodology for in-house fabrication of microtissues with angiogenic potential for downstream use in various tissue regenerative strategies.Multidisciplinary Digital Publishing Institute2022202220212022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion18 p.application/pdfhttps://hdl.handle.net/2445/184454Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.3390/biomedicines9030232Biomedicines, 2021, vol 9, num 3https://doi.org/10.3390/biomedicines9030232cc by (c) Rubi Sans, Gerard et al, 2021http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1844542026-05-29T05:05:01Z
dc.title.none.fl_str_mv Development and Angiogenic Potential of Cell-Derived Microtissues Using Microcarrier-Template
title Development and Angiogenic Potential of Cell-Derived Microtissues Using Microcarrier-Template
spellingShingle Development and Angiogenic Potential of Cell-Derived Microtissues Using Microcarrier-Template
Rubi Sans, Gerard
Bioenginyeria
Medicina regenerativa
Angiogènesi
Bioengineering
Regenerative medicine
Neovascularization
title_short Development and Angiogenic Potential of Cell-Derived Microtissues Using Microcarrier-Template
title_full Development and Angiogenic Potential of Cell-Derived Microtissues Using Microcarrier-Template
title_fullStr Development and Angiogenic Potential of Cell-Derived Microtissues Using Microcarrier-Template
title_full_unstemmed Development and Angiogenic Potential of Cell-Derived Microtissues Using Microcarrier-Template
title_sort Development and Angiogenic Potential of Cell-Derived Microtissues Using Microcarrier-Template
dc.creator.none.fl_str_mv Rubi Sans, Gerard
Cano Torres, Irene
Pérez Amodio, Soledad
Blanco Fernández, Bárbara
Mateos Timoneda, Miguel Ángel
Engel, Elisabeth
author Rubi Sans, Gerard
author_facet Rubi Sans, Gerard
Cano Torres, Irene
Pérez Amodio, Soledad
Blanco Fernández, Bárbara
Mateos Timoneda, Miguel Ángel
Engel, Elisabeth
author_role author
author2 Cano Torres, Irene
Pérez Amodio, Soledad
Blanco Fernández, Bárbara
Mateos Timoneda, Miguel Ángel
Engel, Elisabeth
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Bioenginyeria
Medicina regenerativa
Angiogènesi
Bioengineering
Regenerative medicine
Neovascularization
topic Bioenginyeria
Medicina regenerativa
Angiogènesi
Bioengineering
Regenerative medicine
Neovascularization
description Tissue engineering and regenerative medicine approaches use biomaterials in combination with cells to regenerate lost functions of tissues and organs to prevent organ transplantation. However, most of the current strategies fail in mimicking the tissue's extracellular matrix properties. In order to mimic native tissue conditions, we developed cell-derived matrix (CDM) microtissues (MT). Our methodology uses poly-lactic acid (PLA) and Cultispher(R) S microcarriers' (MCs') as scaffold templates, which are seeded with rat bone marrow mesenchymal stem cells (rBM-MSCs). The scaffold template allows cells to generate an extracellular matrix, which is then extracted for downstream use. The newly formed CDM provides cells with a complex physical (MT architecture) and biochemical (deposited ECM proteins) environment, also showing spontaneous angiogenic potential. Our results suggest that MTs generated from the combination of these two MCs (mixed MTs) are excellent candidates for tissue vascularization. Overall, this study provides a methodology for in-house fabrication of microtissues with angiogenic potential for downstream use in various tissue regenerative strategies.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/184454
url https://hdl.handle.net/2445/184454
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/biomedicines9030232
Biomedicines, 2021, vol 9, num 3
https://doi.org/10.3390/biomedicines9030232
dc.rights.none.fl_str_mv cc by (c) Rubi Sans, Gerard et al, 2021
http://creativecommons.org/licenses/by/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by (c) Rubi Sans, Gerard et al, 2021
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 18 p.
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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