Cathepsin K null mice show reduced adiposity during the rapid accumulation of fat stores

Growing evidences indicate that proteases are implicated in adipogenesis and in the onset of obesity. We previously reported that the cysteine protease cathepsin K (ctsk) is overexpressed in the white adipose tissue (WAT) of obese individuals. We herein characterized the WAT and the metabolic phenot...

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Autores: Funicello, Marcella, Novelli, Michela, Ragni, Maurizio, Vottari, Teresa, Cocuzza, Cesare, López-Soriano, Joaquín, Chiellini, Chiara, Boschi, Federico, Marzola, Pasquina, Masiello, Pellegrino, Saftig, Paul, Santini, Ferruccio, St-Jacques, René, Desmarais, Sylvie, Morin, Nicolas, Mancini, Joseph, Percival, M. David, Pinchera, Aldo, Maffei, Margherita
Formato: artículo
Fecha de publicación:2007
País:España
Recursos:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:109360
Acesso em linha:https://ddd.uab.cat/record/109360
https://dx.doi.org/urn:doi:10.1371/journal.pone.0000683
Access Level:acceso abierto
Palavra-chave:Obesitat
Cysteine protease cathepsin K
White adipose tissue
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spelling Cathepsin K null mice show reduced adiposity during the rapid accumulation of fat storesFunicello, MarcellaNovelli, MichelaRagni, MaurizioVottari, TeresaCocuzza, CesareLópez-Soriano, JoaquínChiellini, ChiaraBoschi, FedericoMarzola, PasquinaMasiello, PellegrinoSaftig, PaulSantini, FerruccioSt-Jacques, RenéDesmarais, SylvieMorin, NicolasMancini, JosephPercival, M. DavidPinchera, AldoMaffei, MargheritaObesitatCysteine protease cathepsin KWhite adipose tissueGrowing evidences indicate that proteases are implicated in adipogenesis and in the onset of obesity. We previously reported that the cysteine protease cathepsin K (ctsk) is overexpressed in the white adipose tissue (WAT) of obese individuals. We herein characterized the WAT and the metabolic phenotype of ctsk deficient animals (ctsk-/-). When the growth rate of ctsk-/- was compared to that of the wild type animals (WT), we could establish a time window (5-8 weeks of age) within which ctsk-/-display significantly lower body weight and WAT size as compared to WT. Such a difference was not observable in older mice. Upon treatment with high fat diet (HFD) for 12 weeks ctsk-/- gained significantly less weight than WT and showed reduced brown adipose tissue, liver mass and a lower percentage of body fat. Plasma triglycerides, cholesterol and leptin were significantly lower in HFD-fed-ctsk-/- as compared to HFD-fed WT animals. Adipocyte lipolysis rates were increased in both young and HFD-fed-ctsk-/-, as compared to WT. Carnitine palmitoyl transferase-1 activity, was higher in mitochondria isolated from the WAT of HFD treated ctsk-/- as compared to WT. Together, these data indicate that ctsk ablation in mice results in reduced body fat content under conditions requiring a rapid accumulation of fat stores. This observation could be partly explained by an increased release and/or utilization of FFA and by an augmented ratio of lipolysis/lipogenesis. These results also demonstrate that under a HFD, ctsk deficiency confers a partial resistance to the development of dyslipidemia. 22007-01-0120072007-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/109360https://dx.doi.org/urn:doi:10.1371/journal.pone.0000683reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/2.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:1093602026-06-06T12:50:31Z
dc.title.none.fl_str_mv Cathepsin K null mice show reduced adiposity during the rapid accumulation of fat stores
title Cathepsin K null mice show reduced adiposity during the rapid accumulation of fat stores
spellingShingle Cathepsin K null mice show reduced adiposity during the rapid accumulation of fat stores
Funicello, Marcella
Obesitat
Cysteine protease cathepsin K
White adipose tissue
title_short Cathepsin K null mice show reduced adiposity during the rapid accumulation of fat stores
title_full Cathepsin K null mice show reduced adiposity during the rapid accumulation of fat stores
title_fullStr Cathepsin K null mice show reduced adiposity during the rapid accumulation of fat stores
title_full_unstemmed Cathepsin K null mice show reduced adiposity during the rapid accumulation of fat stores
title_sort Cathepsin K null mice show reduced adiposity during the rapid accumulation of fat stores
dc.creator.none.fl_str_mv Funicello, Marcella
Novelli, Michela
Ragni, Maurizio
Vottari, Teresa
Cocuzza, Cesare
López-Soriano, Joaquín
Chiellini, Chiara
Boschi, Federico
Marzola, Pasquina
Masiello, Pellegrino
Saftig, Paul
Santini, Ferruccio
St-Jacques, René
Desmarais, Sylvie
Morin, Nicolas
Mancini, Joseph
Percival, M. David
Pinchera, Aldo
Maffei, Margherita
author Funicello, Marcella
author_facet Funicello, Marcella
Novelli, Michela
Ragni, Maurizio
Vottari, Teresa
Cocuzza, Cesare
López-Soriano, Joaquín
Chiellini, Chiara
Boschi, Federico
Marzola, Pasquina
Masiello, Pellegrino
Saftig, Paul
Santini, Ferruccio
St-Jacques, René
Desmarais, Sylvie
Morin, Nicolas
Mancini, Joseph
Percival, M. David
Pinchera, Aldo
Maffei, Margherita
author_role author
author2 Novelli, Michela
Ragni, Maurizio
Vottari, Teresa
Cocuzza, Cesare
López-Soriano, Joaquín
Chiellini, Chiara
Boschi, Federico
Marzola, Pasquina
Masiello, Pellegrino
Saftig, Paul
Santini, Ferruccio
St-Jacques, René
Desmarais, Sylvie
Morin, Nicolas
Mancini, Joseph
Percival, M. David
Pinchera, Aldo
Maffei, Margherita
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Obesitat
Cysteine protease cathepsin K
White adipose tissue
topic Obesitat
Cysteine protease cathepsin K
White adipose tissue
description Growing evidences indicate that proteases are implicated in adipogenesis and in the onset of obesity. We previously reported that the cysteine protease cathepsin K (ctsk) is overexpressed in the white adipose tissue (WAT) of obese individuals. We herein characterized the WAT and the metabolic phenotype of ctsk deficient animals (ctsk-/-). When the growth rate of ctsk-/- was compared to that of the wild type animals (WT), we could establish a time window (5-8 weeks of age) within which ctsk-/-display significantly lower body weight and WAT size as compared to WT. Such a difference was not observable in older mice. Upon treatment with high fat diet (HFD) for 12 weeks ctsk-/- gained significantly less weight than WT and showed reduced brown adipose tissue, liver mass and a lower percentage of body fat. Plasma triglycerides, cholesterol and leptin were significantly lower in HFD-fed-ctsk-/- as compared to HFD-fed WT animals. Adipocyte lipolysis rates were increased in both young and HFD-fed-ctsk-/-, as compared to WT. Carnitine palmitoyl transferase-1 activity, was higher in mitochondria isolated from the WAT of HFD treated ctsk-/- as compared to WT. Together, these data indicate that ctsk ablation in mice results in reduced body fat content under conditions requiring a rapid accumulation of fat stores. This observation could be partly explained by an increased release and/or utilization of FFA and by an augmented ratio of lipolysis/lipogenesis. These results also demonstrate that under a HFD, ctsk deficiency confers a partial resistance to the development of dyslipidemia.
publishDate 2007
dc.date.none.fl_str_mv 2
2007-01-01
2007
2007-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/109360
https://dx.doi.org/urn:doi:10.1371/journal.pone.0000683
url https://ddd.uab.cat/record/109360
https://dx.doi.org/urn:doi:10.1371/journal.pone.0000683
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/2.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/2.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301603

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