Lack of reelin modifies the gene expression in the small intestine of mice

We recently demonstrated that the mucosa of the small intestine of the rat expresses reelin and some components of its signaling system. The current study evaluates whether reelin affects the intestinal gene expression profile using microarray analysis and reeler mice, a natural mutant in which reel...

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Autores: García Miranda, Pablo, Vázquez Carretero, María Dolores, Gutiérrez Pozo, Gabriel, Peral Rubio, María José, Ilundáin Larrañeta, María Anunciación Ana
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2012
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/151981
Acceso en línea:https://hdl.handle.net/11441/151981
https://doi.org/10.1007/s13105-011-0132-0
Access Level:acceso abierto
Palabra clave:Epithelium
Genomic
Immune response
Intestine
Isolated epithelial cells
Reelin
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spelling Lack of reelin modifies the gene expression in the small intestine of miceGarcía Miranda, PabloVázquez Carretero, María DoloresGutiérrez Pozo, GabrielPeral Rubio, María JoséIlundáin Larrañeta, María Anunciación AnaEpitheliumGenomicImmune responseIntestineIsolated epithelial cellsReelinWe recently demonstrated that the mucosa of the small intestine of the rat expresses reelin and some components of its signaling system. The current study evaluates whether reelin affects the intestinal gene expression profile using microarray analysis and reeler mice, a natural mutant in which reelin is not expressed. The effect of the mutation on body weight and intestinal morphology is also evaluated. The mutation reduces body and intestinal weight during the first 2 months of age and modifies the morphology of the crypts and villi. For the microarray assays, total RNA was obtained from either isolated epithelial cells or intact small intestine. Of the 45,101 genes present in the microarray the mutation significantly alters the expression of 62 genes in the isolated epithelial cell samples and of 84 in the intact small intestine. The expression of 83% of the genes tested for validation was substantiated by reverse transcriptase polymerase chain reaction. The mutation notably up-regulates genes involved in intestinal metabolism, while it down-regulates genes related with immune response, inflammation, and tumor development. Genes involved in cell proliferation, differentiation, apoptosis, membrane transport and cytoskeleton are also differently expressed in the reeler mice as compared with the control. This is the first report showing that the lack of reelin modifies intestinal morphology and gene expression profile and suggests a role for reelin in intestinal epithelium homeostasis.Junta de Andalucía CTS 5884Springer NatureFisiologíaGenéticaJunta de Andalucía2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/151981https://doi.org/10.1007/s13105-011-0132-0reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésJournal of Physiology and Biochemistry, 68 (2), 205-218.CTS 5884https://doi.org/10.1007/s13105-011-0132-0info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1519812026-06-17T12:51:07Z
dc.title.none.fl_str_mv Lack of reelin modifies the gene expression in the small intestine of mice
title Lack of reelin modifies the gene expression in the small intestine of mice
spellingShingle Lack of reelin modifies the gene expression in the small intestine of mice
García Miranda, Pablo
Epithelium
Genomic
Immune response
Intestine
Isolated epithelial cells
Reelin
title_short Lack of reelin modifies the gene expression in the small intestine of mice
title_full Lack of reelin modifies the gene expression in the small intestine of mice
title_fullStr Lack of reelin modifies the gene expression in the small intestine of mice
title_full_unstemmed Lack of reelin modifies the gene expression in the small intestine of mice
title_sort Lack of reelin modifies the gene expression in the small intestine of mice
dc.creator.none.fl_str_mv García Miranda, Pablo
Vázquez Carretero, María Dolores
Gutiérrez Pozo, Gabriel
Peral Rubio, María José
Ilundáin Larrañeta, María Anunciación Ana
author García Miranda, Pablo
author_facet García Miranda, Pablo
Vázquez Carretero, María Dolores
Gutiérrez Pozo, Gabriel
Peral Rubio, María José
Ilundáin Larrañeta, María Anunciación Ana
author_role author
author2 Vázquez Carretero, María Dolores
Gutiérrez Pozo, Gabriel
Peral Rubio, María José
Ilundáin Larrañeta, María Anunciación Ana
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Fisiología
Genética
Junta de Andalucía
dc.subject.none.fl_str_mv Epithelium
Genomic
Immune response
Intestine
Isolated epithelial cells
Reelin
topic Epithelium
Genomic
Immune response
Intestine
Isolated epithelial cells
Reelin
description We recently demonstrated that the mucosa of the small intestine of the rat expresses reelin and some components of its signaling system. The current study evaluates whether reelin affects the intestinal gene expression profile using microarray analysis and reeler mice, a natural mutant in which reelin is not expressed. The effect of the mutation on body weight and intestinal morphology is also evaluated. The mutation reduces body and intestinal weight during the first 2 months of age and modifies the morphology of the crypts and villi. For the microarray assays, total RNA was obtained from either isolated epithelial cells or intact small intestine. Of the 45,101 genes present in the microarray the mutation significantly alters the expression of 62 genes in the isolated epithelial cell samples and of 84 in the intact small intestine. The expression of 83% of the genes tested for validation was substantiated by reverse transcriptase polymerase chain reaction. The mutation notably up-regulates genes involved in intestinal metabolism, while it down-regulates genes related with immune response, inflammation, and tumor development. Genes involved in cell proliferation, differentiation, apoptosis, membrane transport and cytoskeleton are also differently expressed in the reeler mice as compared with the control. This is the first report showing that the lack of reelin modifies intestinal morphology and gene expression profile and suggests a role for reelin in intestinal epithelium homeostasis.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/151981
https://doi.org/10.1007/s13105-011-0132-0
url https://hdl.handle.net/11441/151981
https://doi.org/10.1007/s13105-011-0132-0
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Journal of Physiology and Biochemistry, 68 (2), 205-218.
CTS 5884
https://doi.org/10.1007/s13105-011-0132-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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