DNA methylation and histone acetylation of rat methionine adenosyltransferase 1A and 2A genes is tissue-specific

Torres, L. (Luis)|||/items/e4740a05-e8bb-4f09-b3ca-e119dae6742b; Lopez-Rodas, G. (Gerardo)|||/items/32cdfe1e-70b1-48f3-8992-236a1d17bb83; Latasa, M.U. (María Ujué)|||/items/e1e74596-1598-4722-a96a-7bdd7cdec356; Carretero, M.V. (M. Victoria)|||/items/32989e88-e020-4ae5-befe-dcd0b529664b; Boukaba, A. (Abdelhalim)|||/items/354fa8ac-2569-4178-a064-1fa9cc79ddca; Rodriguez, J.L. (José L.)|||/items/fa2733a5-c7c0-4e99-9d41-64ee4cf1c54f; Franco, L. (Luis)|||/items/3ca4ede0-fdd2-4a83-a72f-b90cf9570816; Mato, J.M. (José María)|||/items/302dc624-b0d3-4703-90cf-1a97690ebc79; Avila, M.A. (Matías Antonio)|||/items/3ad9abbb-c18d-445b-86cf-cb76be15419f

DNA methylation and histone acetylation of rat methionine adenosyltransferase 1A and 2A genes is tissue-specific

Methionine adenosyltransferase (MAT) catalyzes the biosynthesis of S-adenosylmethionine (AdoMet). In mammals MAT activity derives from two separate genes which display a tissue-specific pattern of expression. While MAT1A is expressed only in the adult liver, MAT2A is expressed in non-hepatic tissues...

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Detalles Bibliográficos
Autores:	Torres, L. (Luis)\|\|\|/items/e4740a05-e8bb-4f09-b3ca-e119dae6742b, Lopez-Rodas, G. (Gerardo)\|\|\|/items/32cdfe1e-70b1-48f3-8992-236a1d17bb83, Latasa, M.U. (María Ujué)\|\|\|/items/e1e74596-1598-4722-a96a-7bdd7cdec356, Carretero, M.V. (M. Victoria)\|\|\|/items/32989e88-e020-4ae5-befe-dcd0b529664b, Boukaba, A. (Abdelhalim)\|\|\|/items/354fa8ac-2569-4178-a064-1fa9cc79ddca, Rodriguez, J.L. (José L.)\|\|\|/items/fa2733a5-c7c0-4e99-9d41-64ee4cf1c54f, Franco, L. (Luis)\|\|\|/items/3ca4ede0-fdd2-4a83-a72f-b90cf9570816, Mato, J.M. (José María)\|\|\|/items/302dc624-b0d3-4703-90cf-1a97690ebc79, Avila, M.A. (Matías Antonio)\|\|\|/items/3ad9abbb-c18d-445b-86cf-cb76be15419f
Tipo de recurso:	artículo
Fecha de publicación:	2000
País:	España
Institución:	Universidad de Navarra
Repositorio:	Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:	inglés
OAI Identifier:	oai:dadun.unav.edu:10171/23251
Acceso en línea:	https://hdl.handle.net/10171/23251
Access Level:	acceso abierto
Palabra clave:	Methionine adenosyltransferase Gene expression DNA methylation Histone acetylation Anti-acetylated H4

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spelling	DNA methylation and histone acetylation of rat methionine adenosyltransferase 1A and 2A genes is tissue-specificTorres, L. (Luis)\|\|\|/items/e4740a05-e8bb-4f09-b3ca-e119dae6742bLopez-Rodas, G. (Gerardo)\|\|\|/items/32cdfe1e-70b1-48f3-8992-236a1d17bb83Latasa, M.U. (María Ujué)\|\|\|/items/e1e74596-1598-4722-a96a-7bdd7cdec356Carretero, M.V. (M. Victoria)\|\|\|/items/32989e88-e020-4ae5-befe-dcd0b529664bBoukaba, A. (Abdelhalim)\|\|\|/items/354fa8ac-2569-4178-a064-1fa9cc79ddcaRodriguez, J.L. (José L.)\|\|\|/items/fa2733a5-c7c0-4e99-9d41-64ee4cf1c54fFranco, L. (Luis)\|\|\|/items/3ca4ede0-fdd2-4a83-a72f-b90cf9570816Mato, J.M. (José María)\|\|\|/items/302dc624-b0d3-4703-90cf-1a97690ebc79Avila, M.A. (Matías Antonio)\|\|\|/items/3ad9abbb-c18d-445b-86cf-cb76be15419fMethionine adenosyltransferaseGene expressionDNA methylationHistone acetylationAnti-acetylated H4Methionine adenosyltransferase (MAT) catalyzes the biosynthesis of S-adenosylmethionine (AdoMet). In mammals MAT activity derives from two separate genes which display a tissue-specific pattern of expression. While MAT1A is expressed only in the adult liver, MAT2A is expressed in non-hepatic tissues. The mechanisms behind the selective expression of these two genes are not fully understood. In the present report we have evaluated MAT1A and MAT2A methylation in liver and in other tissues, such as kidney, by methylation-sensitive restriction enzyme digestion of genomic DNA. Our data indicate that MAT1A is hypomethylated in liver and hypermethylated in non-expressing tissues. The opposite situation is found for MAT2A. Additionally, histones associated to MAT1A and MAT2A genes showed enhanced levels of acetylation in expressing tissues (two-fold for MAT1A and 3.5-fold for MAT2A liver and kidney respectively). These observations support a role for chromatin structure and its modification in the tissue-specific expression of both MAT genes.ElsevierDadun. Depósito Académico Digital Universidad de Navarra20122012-09-2820002000-01-0120002000-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/23251reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/232512026-06-21T12:47:57Z
dc.title.none.fl_str_mv	DNA methylation and histone acetylation of rat methionine adenosyltransferase 1A and 2A genes is tissue-specific
title	DNA methylation and histone acetylation of rat methionine adenosyltransferase 1A and 2A genes is tissue-specific
spellingShingle	DNA methylation and histone acetylation of rat methionine adenosyltransferase 1A and 2A genes is tissue-specific Torres, L. (Luis)\|\|\|/items/e4740a05-e8bb-4f09-b3ca-e119dae6742b Methionine adenosyltransferase Gene expression DNA methylation Histone acetylation Anti-acetylated H4
title_short	DNA methylation and histone acetylation of rat methionine adenosyltransferase 1A and 2A genes is tissue-specific
title_full	DNA methylation and histone acetylation of rat methionine adenosyltransferase 1A and 2A genes is tissue-specific
title_fullStr	DNA methylation and histone acetylation of rat methionine adenosyltransferase 1A and 2A genes is tissue-specific
title_full_unstemmed	DNA methylation and histone acetylation of rat methionine adenosyltransferase 1A and 2A genes is tissue-specific
title_sort	DNA methylation and histone acetylation of rat methionine adenosyltransferase 1A and 2A genes is tissue-specific
dc.creator.none.fl_str_mv	Torres, L. (Luis)\|\|\|/items/e4740a05-e8bb-4f09-b3ca-e119dae6742b Lopez-Rodas, G. (Gerardo)\|\|\|/items/32cdfe1e-70b1-48f3-8992-236a1d17bb83 Latasa, M.U. (María Ujué)\|\|\|/items/e1e74596-1598-4722-a96a-7bdd7cdec356 Carretero, M.V. (M. Victoria)\|\|\|/items/32989e88-e020-4ae5-befe-dcd0b529664b Boukaba, A. (Abdelhalim)\|\|\|/items/354fa8ac-2569-4178-a064-1fa9cc79ddca Rodriguez, J.L. (José L.)\|\|\|/items/fa2733a5-c7c0-4e99-9d41-64ee4cf1c54f Franco, L. (Luis)\|\|\|/items/3ca4ede0-fdd2-4a83-a72f-b90cf9570816 Mato, J.M. (José María)\|\|\|/items/302dc624-b0d3-4703-90cf-1a97690ebc79 Avila, M.A. (Matías Antonio)\|\|\|/items/3ad9abbb-c18d-445b-86cf-cb76be15419f
author	Torres, L. (Luis)\|\|\|/items/e4740a05-e8bb-4f09-b3ca-e119dae6742b
author_facet	Torres, L. (Luis)\|\|\|/items/e4740a05-e8bb-4f09-b3ca-e119dae6742b Lopez-Rodas, G. (Gerardo)\|\|\|/items/32cdfe1e-70b1-48f3-8992-236a1d17bb83 Latasa, M.U. (María Ujué)\|\|\|/items/e1e74596-1598-4722-a96a-7bdd7cdec356 Carretero, M.V. (M. Victoria)\|\|\|/items/32989e88-e020-4ae5-befe-dcd0b529664b Boukaba, A. (Abdelhalim)\|\|\|/items/354fa8ac-2569-4178-a064-1fa9cc79ddca Rodriguez, J.L. (José L.)\|\|\|/items/fa2733a5-c7c0-4e99-9d41-64ee4cf1c54f Franco, L. (Luis)\|\|\|/items/3ca4ede0-fdd2-4a83-a72f-b90cf9570816 Mato, J.M. (José María)\|\|\|/items/302dc624-b0d3-4703-90cf-1a97690ebc79 Avila, M.A. (Matías Antonio)\|\|\|/items/3ad9abbb-c18d-445b-86cf-cb76be15419f
author_role	author
author2	Lopez-Rodas, G. (Gerardo)\|\|\|/items/32cdfe1e-70b1-48f3-8992-236a1d17bb83 Latasa, M.U. (María Ujué)\|\|\|/items/e1e74596-1598-4722-a96a-7bdd7cdec356 Carretero, M.V. (M. Victoria)\|\|\|/items/32989e88-e020-4ae5-befe-dcd0b529664b Boukaba, A. (Abdelhalim)\|\|\|/items/354fa8ac-2569-4178-a064-1fa9cc79ddca Rodriguez, J.L. (José L.)\|\|\|/items/fa2733a5-c7c0-4e99-9d41-64ee4cf1c54f Franco, L. (Luis)\|\|\|/items/3ca4ede0-fdd2-4a83-a72f-b90cf9570816 Mato, J.M. (José María)\|\|\|/items/302dc624-b0d3-4703-90cf-1a97690ebc79 Avila, M.A. (Matías Antonio)\|\|\|/items/3ad9abbb-c18d-445b-86cf-cb76be15419f
author2_role	author author author author author author author author
dc.contributor.none.fl_str_mv	Dadun. Depósito Académico Digital Universidad de Navarra
dc.subject.none.fl_str_mv	Methionine adenosyltransferase Gene expression DNA methylation Histone acetylation Anti-acetylated H4
topic	Methionine adenosyltransferase Gene expression DNA methylation Histone acetylation Anti-acetylated H4
description	Methionine adenosyltransferase (MAT) catalyzes the biosynthesis of S-adenosylmethionine (AdoMet). In mammals MAT activity derives from two separate genes which display a tissue-specific pattern of expression. While MAT1A is expressed only in the adult liver, MAT2A is expressed in non-hepatic tissues. The mechanisms behind the selective expression of these two genes are not fully understood. In the present report we have evaluated MAT1A and MAT2A methylation in liver and in other tissues, such as kidney, by methylation-sensitive restriction enzyme digestion of genomic DNA. Our data indicate that MAT1A is hypomethylated in liver and hypermethylated in non-expressing tissues. The opposite situation is found for MAT2A. Additionally, histones associated to MAT1A and MAT2A genes showed enhanced levels of acetylation in expressing tissues (two-fold for MAT1A and 3.5-fold for MAT2A liver and kidney respectively). These observations support a role for chromatin structure and its modification in the tissue-specific expression of both MAT genes.
publishDate	2000
dc.date.none.fl_str_mv	2000 2000-01-01 2000 2000-01-01 2012 2012-09-28
dc.type.none.fl_str_mv	journal article http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	https://hdl.handle.net/10171/23251
url	https://hdl.handle.net/10171/23251
dc.language.none.fl_str_mv	Inglés eng
language_invalid_str_mv	Inglés
language	eng
dc.rights.none.fl_str_mv	open access http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv	info:eu-repo/semantics/openAccess
rights_invalid_str_mv	open access http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.publisher.none.fl_str_mv	Elsevier
publisher.none.fl_str_mv	Elsevier
dc.source.none.fl_str_mv	reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra instname:Universidad de Navarra
instname_str	Universidad de Navarra
reponame_str	Dadun. Depósito Académico Digital de la Universidad de Navarra
collection	Dadun. Depósito Académico Digital de la Universidad de Navarra
repository.name.fl_str_mv
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DNA methylation and histone acetylation of rat methionine adenosyltransferase 1A and 2A genes is tissue-specific

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