Field evaluation of the P22 ELISA for diagnosis of caprine tuberculosis in an endemic area

Animal tuberculosis (TB) affects a wide range of domestic species, including goats. TB eradication programs in goats are based on cell-based techniques such as the single and comparative intradermal tuberculin test (SITT and CITT, respectively). In recent years, an ELISA technique based on the P22 p...

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Autores: Velasco, Carlos, Ortega, Javier, Álvarez Sánchez, Julio, Infantes Lorenzo, Jose Antonio, Moreno, José C., Sanz, Cristina, Romero Martínez, Beatriz, Juan Ferré, Lucía De, Domínguez Rodríguez, Lucas José, Dominguez, Mercedes, Moreno, Inmaculada, Roy, Alvaro, Bezos Garrido, Javier
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/123186
Acceso en línea:https://hdl.handle.net/20.500.14352/123186
Access Level:acceso abierto
Palabra clave:579.62
Goat
Tuberculosis
Intradermal tuberculin test
P22 ELISA
Serum
Milk
Mulk tank milk
Microbiología (Veterinaria)
3109 Ciencias Veterinarias
3109.05 Microbiología
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repository_id_str
spelling Field evaluation of the P22 ELISA for diagnosis of caprine tuberculosis in an endemic areaVelasco, CarlosOrtega, JavierÁlvarez Sánchez, JulioInfantes Lorenzo, Jose AntonioMoreno, José C.Sanz, CristinaRomero Martínez, BeatrizJuan Ferré, Lucía DeDomínguez Rodríguez, Lucas JoséDominguez, MercedesMoreno, InmaculadaRoy, AlvaroBezos Garrido, Javier579.62GoatTuberculosisIntradermal tuberculin testP22 ELISASerumMilkMulk tank milkMicrobiología (Veterinaria)3109 Ciencias Veterinarias3109.05 MicrobiologíaAnimal tuberculosis (TB) affects a wide range of domestic species, including goats. TB eradication programs in goats are based on cell-based techniques such as the single and comparative intradermal tuberculin test (SITT and CITT, respectively). In recent years, an ELISA technique based on the P22 protein complex (P22 ELISA), has emerged as a valuable tool for TB diagnosis. The aim of the study was to evaluate the performance of the P22 ELISA in the context of a caprine TB eradication program using serum, individual milk and bulk tank milk (BTM) samples in order to define its usefulness in classifying herds compared to SITT and CITT. Samples from 53 herds categorized based on the detection of CITT reactors (16 high-risk herds, with one or more CITT reactors, and 37 low-risk herds, with only CITT-negative goats) were analyzed. Reactors in the P22 ELISA were detected in a higher number of high-risk herds using both serum (87.5%) and individual milk (81.3%) compared to SITT (75.0%) and CITT (31.3%), while the use of BTM led to the detection of 33.3% of the herds. Individual apparent prevalence was higher using the P22 ELISA in both serum (11.0%) and milk (15.0%) compared to the SITT (6.8%) and CITT (2.5%), with also a significantly (p < 0.001) higher number of reactors in individual milk compared with the serum. Similarly, all six herds with MTBC confirmed infection showed reactors to the SITT, CITT, and individual serum and milk P22 ELISA (2 out of 5 detected using BTM), although the highest reactivity was observed using individual milk samples. In the low-risk herds, a lower number of positive herds and animals were found with the P22 ELISA using serum or individual milk (51.4%) compared to SITT (59.5%) while using CITT only 2.7% of the herds were positive and none reacted to the P22 ELISA in BTM samples. This study shows that the P22 ELISA, using serum and especially individual milk samples, could be a complementary tool for maximizing the sensitivity of intradermal testing within the framework of a caprine TB eradication program.Frontiers MediaUniversidad Complutense de Madrid20252025-01-0120252025-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/123186reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023 PCI2023-143368 IMPROVING THE DIAGNOSIS OF TUBERCULOSIS IN DOMESTIC RUMINANTS THROUGH THE USE OF NEW ANTIGENS AND TEST PLATFORMSopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1231862026-06-02T12:44:21Z
dc.title.none.fl_str_mv Field evaluation of the P22 ELISA for diagnosis of caprine tuberculosis in an endemic area
title Field evaluation of the P22 ELISA for diagnosis of caprine tuberculosis in an endemic area
spellingShingle Field evaluation of the P22 ELISA for diagnosis of caprine tuberculosis in an endemic area
Velasco, Carlos
579.62
Goat
Tuberculosis
Intradermal tuberculin test
P22 ELISA
Serum
Milk
Mulk tank milk
Microbiología (Veterinaria)
3109 Ciencias Veterinarias
3109.05 Microbiología
title_short Field evaluation of the P22 ELISA for diagnosis of caprine tuberculosis in an endemic area
title_full Field evaluation of the P22 ELISA for diagnosis of caprine tuberculosis in an endemic area
title_fullStr Field evaluation of the P22 ELISA for diagnosis of caprine tuberculosis in an endemic area
title_full_unstemmed Field evaluation of the P22 ELISA for diagnosis of caprine tuberculosis in an endemic area
title_sort Field evaluation of the P22 ELISA for diagnosis of caprine tuberculosis in an endemic area
dc.creator.none.fl_str_mv Velasco, Carlos
Ortega, Javier
Álvarez Sánchez, Julio
Infantes Lorenzo, Jose Antonio
Moreno, José C.
Sanz, Cristina
Romero Martínez, Beatriz
Juan Ferré, Lucía De
Domínguez Rodríguez, Lucas José
Dominguez, Mercedes
Moreno, Inmaculada
Roy, Alvaro
Bezos Garrido, Javier
author Velasco, Carlos
author_facet Velasco, Carlos
Ortega, Javier
Álvarez Sánchez, Julio
Infantes Lorenzo, Jose Antonio
Moreno, José C.
Sanz, Cristina
Romero Martínez, Beatriz
Juan Ferré, Lucía De
Domínguez Rodríguez, Lucas José
Dominguez, Mercedes
Moreno, Inmaculada
Roy, Alvaro
Bezos Garrido, Javier
author_role author
author2 Ortega, Javier
Álvarez Sánchez, Julio
Infantes Lorenzo, Jose Antonio
Moreno, José C.
Sanz, Cristina
Romero Martínez, Beatriz
Juan Ferré, Lucía De
Domínguez Rodríguez, Lucas José
Dominguez, Mercedes
Moreno, Inmaculada
Roy, Alvaro
Bezos Garrido, Javier
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 579.62
Goat
Tuberculosis
Intradermal tuberculin test
P22 ELISA
Serum
Milk
Mulk tank milk
Microbiología (Veterinaria)
3109 Ciencias Veterinarias
3109.05 Microbiología
topic 579.62
Goat
Tuberculosis
Intradermal tuberculin test
P22 ELISA
Serum
Milk
Mulk tank milk
Microbiología (Veterinaria)
3109 Ciencias Veterinarias
3109.05 Microbiología
description Animal tuberculosis (TB) affects a wide range of domestic species, including goats. TB eradication programs in goats are based on cell-based techniques such as the single and comparative intradermal tuberculin test (SITT and CITT, respectively). In recent years, an ELISA technique based on the P22 protein complex (P22 ELISA), has emerged as a valuable tool for TB diagnosis. The aim of the study was to evaluate the performance of the P22 ELISA in the context of a caprine TB eradication program using serum, individual milk and bulk tank milk (BTM) samples in order to define its usefulness in classifying herds compared to SITT and CITT. Samples from 53 herds categorized based on the detection of CITT reactors (16 high-risk herds, with one or more CITT reactors, and 37 low-risk herds, with only CITT-negative goats) were analyzed. Reactors in the P22 ELISA were detected in a higher number of high-risk herds using both serum (87.5%) and individual milk (81.3%) compared to SITT (75.0%) and CITT (31.3%), while the use of BTM led to the detection of 33.3% of the herds. Individual apparent prevalence was higher using the P22 ELISA in both serum (11.0%) and milk (15.0%) compared to the SITT (6.8%) and CITT (2.5%), with also a significantly (p < 0.001) higher number of reactors in individual milk compared with the serum. Similarly, all six herds with MTBC confirmed infection showed reactors to the SITT, CITT, and individual serum and milk P22 ELISA (2 out of 5 detected using BTM), although the highest reactivity was observed using individual milk samples. In the low-risk herds, a lower number of positive herds and animals were found with the P22 ELISA using serum or individual milk (51.4%) compared to SITT (59.5%) while using CITT only 2.7% of the herds were positive and none reacted to the P22 ELISA in BTM samples. This study shows that the P22 ELISA, using serum and especially individual milk samples, could be a complementary tool for maximizing the sensitivity of intradermal testing within the framework of a caprine TB eradication program.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025-01-01
2025
2025-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/123186
url https://hdl.handle.net/20.500.14352/123186
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023 PCI2023-143368 IMPROVING THE DIAGNOSIS OF TUBERCULOSIS IN DOMESTIC RUMINANTS THROUGH THE USE OF NEW ANTIGENS AND TEST PLATFORMS
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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