NCS-1 Inhibits insulin stimulated GLUT4 translocation in 3T3L1 adipocytes through a phosphatidylinositol 4-kinase dependent pathway

Expression of NCS-1 (neuronal calcium sensor-1, also termed frequenin) in 3T3L1 adipocytes strongly inhibited insulin-stimulated translocation of GLUT4 and insulin-responsive aminopeptidase. The effect of NCS-1 was specific for GLUT4 and the insulin-responsive aminopeptidase translocation as there w...

Descripción completa

Detalles Bibliográficos
Autores: Mora Fayos, Sílvia, Durham, Paul L., Smith, Jeffery R., Russo, Andrew F., Jeromin, Andrea, Pessin, Jeffrey E.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2002
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/176737
Acceso en línea:https://hdl.handle.net/2445/176737
Access Level:acceso abierto
Palabra clave:Fosforilació
Teixit adipós
Receptors d'insulina
Phosphorylation
Adipose tissues
Insulin receptors
id ES_d4e0b701dc1afdd2b215d5728d322bdf
oai_identifier_str oai:recercat.cat:2445/176737
network_acronym_str ES
network_name_str España
repository_id_str
spelling NCS-1 Inhibits insulin stimulated GLUT4 translocation in 3T3L1 adipocytes through a phosphatidylinositol 4-kinase dependent pathwayMora Fayos, SílviaDurham, Paul L.Smith, Jeffery R.Russo, Andrew F.Jeromin, AndreaPessin, Jeffrey E.FosforilacióTeixit adipósReceptors d'insulinaPhosphorylationAdipose tissuesInsulin receptorsExpression of NCS-1 (neuronal calcium sensor-1, also termed frequenin) in 3T3L1 adipocytes strongly inhibited insulin-stimulated translocation of GLUT4 and insulin-responsive aminopeptidase. The effect of NCS-1 was specific for GLUT4 and the insulin-responsive aminopeptidase translocation as there was no effect on the trafficking of the cation-independent mannose 6-phosphate receptor or the GLUT1 glucose transporter isoform. Moreover, NCS-1 showed partial colocalization with GLUT4-EGFP in the perinuclear region. The inhibitory action of NCS-1 was independent of calcium sequestration since neither treatment with ionomycin nor endothelin-1, both of which elevated the intracellular calcium concentration, restored insulin-stimulated GLUT4 translocation. Furthermore, NCS-1 did not alter the insulin-stimulated protein kinase B (PKB/Akt) phosphorylation or the recruitment of Cbl to the plasma membrane. In contrast, expression of the NCS-1 effector phosphatidylinositol 4-kinase (PI 4-kinase) inhibited insulin-stimulated GLUT4 translocation, whereas co-transfection with an inactive PI 4-kinase mutant prevented the NCS-1-induced inhibition. These data demonstrate that PI 4-kinase functions to negatively regulate GLUT4 translocation through its interaction with NCS-1.American Society for Biochemistry and Molecular Biology2021202120022021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion7 p.application/pdfhttps://hdl.handle.net/2445/176737Articles publicats en revistes (Bioquímica i Biomedicina Molecular)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1074/jbc.M203669200Journal of Biological Chemistry, 2002, vol. 277, p. 27494-27500https://doi.org/10.1074/jbc.M203669200(c) American Society for Biochemistry and Molecular Biology, 2002info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1767372026-05-29T05:05:01Z
dc.title.none.fl_str_mv NCS-1 Inhibits insulin stimulated GLUT4 translocation in 3T3L1 adipocytes through a phosphatidylinositol 4-kinase dependent pathway
title NCS-1 Inhibits insulin stimulated GLUT4 translocation in 3T3L1 adipocytes through a phosphatidylinositol 4-kinase dependent pathway
spellingShingle NCS-1 Inhibits insulin stimulated GLUT4 translocation in 3T3L1 adipocytes through a phosphatidylinositol 4-kinase dependent pathway
Mora Fayos, Sílvia
Fosforilació
Teixit adipós
Receptors d'insulina
Phosphorylation
Adipose tissues
Insulin receptors
title_short NCS-1 Inhibits insulin stimulated GLUT4 translocation in 3T3L1 adipocytes through a phosphatidylinositol 4-kinase dependent pathway
title_full NCS-1 Inhibits insulin stimulated GLUT4 translocation in 3T3L1 adipocytes through a phosphatidylinositol 4-kinase dependent pathway
title_fullStr NCS-1 Inhibits insulin stimulated GLUT4 translocation in 3T3L1 adipocytes through a phosphatidylinositol 4-kinase dependent pathway
title_full_unstemmed NCS-1 Inhibits insulin stimulated GLUT4 translocation in 3T3L1 adipocytes through a phosphatidylinositol 4-kinase dependent pathway
title_sort NCS-1 Inhibits insulin stimulated GLUT4 translocation in 3T3L1 adipocytes through a phosphatidylinositol 4-kinase dependent pathway
dc.creator.none.fl_str_mv Mora Fayos, Sílvia
Durham, Paul L.
Smith, Jeffery R.
Russo, Andrew F.
Jeromin, Andrea
Pessin, Jeffrey E.
author Mora Fayos, Sílvia
author_facet Mora Fayos, Sílvia
Durham, Paul L.
Smith, Jeffery R.
Russo, Andrew F.
Jeromin, Andrea
Pessin, Jeffrey E.
author_role author
author2 Durham, Paul L.
Smith, Jeffery R.
Russo, Andrew F.
Jeromin, Andrea
Pessin, Jeffrey E.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Fosforilació
Teixit adipós
Receptors d'insulina
Phosphorylation
Adipose tissues
Insulin receptors
topic Fosforilació
Teixit adipós
Receptors d'insulina
Phosphorylation
Adipose tissues
Insulin receptors
description Expression of NCS-1 (neuronal calcium sensor-1, also termed frequenin) in 3T3L1 adipocytes strongly inhibited insulin-stimulated translocation of GLUT4 and insulin-responsive aminopeptidase. The effect of NCS-1 was specific for GLUT4 and the insulin-responsive aminopeptidase translocation as there was no effect on the trafficking of the cation-independent mannose 6-phosphate receptor or the GLUT1 glucose transporter isoform. Moreover, NCS-1 showed partial colocalization with GLUT4-EGFP in the perinuclear region. The inhibitory action of NCS-1 was independent of calcium sequestration since neither treatment with ionomycin nor endothelin-1, both of which elevated the intracellular calcium concentration, restored insulin-stimulated GLUT4 translocation. Furthermore, NCS-1 did not alter the insulin-stimulated protein kinase B (PKB/Akt) phosphorylation or the recruitment of Cbl to the plasma membrane. In contrast, expression of the NCS-1 effector phosphatidylinositol 4-kinase (PI 4-kinase) inhibited insulin-stimulated GLUT4 translocation, whereas co-transfection with an inactive PI 4-kinase mutant prevented the NCS-1-induced inhibition. These data demonstrate that PI 4-kinase functions to negatively regulate GLUT4 translocation through its interaction with NCS-1.
publishDate 2002
dc.date.none.fl_str_mv 2002
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/176737
url https://hdl.handle.net/2445/176737
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1074/jbc.M203669200
Journal of Biological Chemistry, 2002, vol. 277, p. 27494-27500
https://doi.org/10.1074/jbc.M203669200
dc.rights.none.fl_str_mv (c) American Society for Biochemistry and Molecular Biology, 2002
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) American Society for Biochemistry and Molecular Biology, 2002
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 7 p.
application/pdf
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Biomedicina Molecular)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869420584736129024
score 15,81155

Similares en LA Referencia