The Relation Between Protein Adsorption and Hemocompatibility of Antifouling Polymer Brushes

Whenever an artificial surface comes into contact with blood, proteins are rapidly adsorbed onto its surface. This phenomenon, termed fouling, is then followed by a series of undesired reactions involving activation of complement or the coagulation cascade and adhesion of leukocytes and platelets le...

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Autores: Riedelová, Zuzana, Santos Pereira, Andres de los, Svoboda, Jan, Pop Georgievski, Ognen, Májek, Pavel, Pečánková, Klára, Dyčka, Filip, Rodriguez Emmenegger, César, Riedel, Tomáš
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2022
País:España
Recursos:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/197645
Acesso em linha:https://hdl.handle.net/2445/197645
Access Level:acceso abierto
Palavra-chave:Trombosi
Materials biomèdics
Polímers en medicina
Thrombosis
Biomedical materials
Polymers in medicine
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spelling The Relation Between Protein Adsorption and Hemocompatibility of Antifouling Polymer BrushesRiedelová, ZuzanaSantos Pereira, Andres de losSvoboda, JanPop Georgievski, OgnenMájek, PavelPečánková, KláraDyčka, FilipRodriguez Emmenegger, CésarRiedel, TomášTrombosiMaterials biomèdicsPolímers en medicinaThrombosisBiomedical materialsPolymers in medicineWhenever an artificial surface comes into contact with blood, proteins are rapidly adsorbed onto its surface. This phenomenon, termed fouling, is then followed by a series of undesired reactions involving activation of complement or the coagulation cascade and adhesion of leukocytes and platelets leading to thrombus formation. Thus, considerable efforts are directed towards the preparation of fouling-resistant surfaces with the best possible hemocompatibility. Herein, a comprehensive hemocompatibility study after heparinized blood contact with seven polymer brushes prepared by surface-initiated atom transfer radical polymerization is reported. The resistance to fouling is quantified and thrombus formation and deposition of blood cellular components on the coatings are analyzed. Moreover, identification of the remaining adsorbed proteins is performed via mass spectroscopy to elucidate their influence on the surface hemocompatibility. Compared with an unmodified glass surface, the grafting of polymer brushes minimizes the adhesion of platelets and leukocytes and prevents the thrombus formation. The fouling from undiluted blood plasma is reduced by up to 99%. Most of the identified proteins are connected with the initial events of foreign body reaction towards biomaterial (coagulation cascade proteins, complement component, and inflammatory proteins). In addition, several proteins that are not previously linked with blood-biomaterial interaction are presented and discussed.Wiley2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/197645Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésVersió postprint del document publicat a: https://doi.org/10.1002/mabi.202200247Macromolecular Bioscience, 2022, vol. 22, num. 11, p. 2200247https://doi.org/10.1002/mabi.202200247(c) John Wiley & Sons, Inc, 2022info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1976452026-05-27T06:46:51Z
dc.title.none.fl_str_mv The Relation Between Protein Adsorption and Hemocompatibility of Antifouling Polymer Brushes
title The Relation Between Protein Adsorption and Hemocompatibility of Antifouling Polymer Brushes
spellingShingle The Relation Between Protein Adsorption and Hemocompatibility of Antifouling Polymer Brushes
Riedelová, Zuzana
Trombosi
Materials biomèdics
Polímers en medicina
Thrombosis
Biomedical materials
Polymers in medicine
title_short The Relation Between Protein Adsorption and Hemocompatibility of Antifouling Polymer Brushes
title_full The Relation Between Protein Adsorption and Hemocompatibility of Antifouling Polymer Brushes
title_fullStr The Relation Between Protein Adsorption and Hemocompatibility of Antifouling Polymer Brushes
title_full_unstemmed The Relation Between Protein Adsorption and Hemocompatibility of Antifouling Polymer Brushes
title_sort The Relation Between Protein Adsorption and Hemocompatibility of Antifouling Polymer Brushes
dc.creator.none.fl_str_mv Riedelová, Zuzana
Santos Pereira, Andres de los
Svoboda, Jan
Pop Georgievski, Ognen
Májek, Pavel
Pečánková, Klára
Dyčka, Filip
Rodriguez Emmenegger, César
Riedel, Tomáš
author Riedelová, Zuzana
author_facet Riedelová, Zuzana
Santos Pereira, Andres de los
Svoboda, Jan
Pop Georgievski, Ognen
Májek, Pavel
Pečánková, Klára
Dyčka, Filip
Rodriguez Emmenegger, César
Riedel, Tomáš
author_role author
author2 Santos Pereira, Andres de los
Svoboda, Jan
Pop Georgievski, Ognen
Májek, Pavel
Pečánková, Klára
Dyčka, Filip
Rodriguez Emmenegger, César
Riedel, Tomáš
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Trombosi
Materials biomèdics
Polímers en medicina
Thrombosis
Biomedical materials
Polymers in medicine
topic Trombosi
Materials biomèdics
Polímers en medicina
Thrombosis
Biomedical materials
Polymers in medicine
description Whenever an artificial surface comes into contact with blood, proteins are rapidly adsorbed onto its surface. This phenomenon, termed fouling, is then followed by a series of undesired reactions involving activation of complement or the coagulation cascade and adhesion of leukocytes and platelets leading to thrombus formation. Thus, considerable efforts are directed towards the preparation of fouling-resistant surfaces with the best possible hemocompatibility. Herein, a comprehensive hemocompatibility study after heparinized blood contact with seven polymer brushes prepared by surface-initiated atom transfer radical polymerization is reported. The resistance to fouling is quantified and thrombus formation and deposition of blood cellular components on the coatings are analyzed. Moreover, identification of the remaining adsorbed proteins is performed via mass spectroscopy to elucidate their influence on the surface hemocompatibility. Compared with an unmodified glass surface, the grafting of polymer brushes minimizes the adhesion of platelets and leukocytes and prevents the thrombus formation. The fouling from undiluted blood plasma is reduced by up to 99%. Most of the identified proteins are connected with the initial events of foreign body reaction towards biomaterial (coagulation cascade proteins, complement component, and inflammatory proteins). In addition, several proteins that are not previously linked with blood-biomaterial interaction are presented and discussed.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/197645
url https://hdl.handle.net/2445/197645
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1002/mabi.202200247
Macromolecular Bioscience, 2022, vol. 22, num. 11, p. 2200247
https://doi.org/10.1002/mabi.202200247
dc.rights.none.fl_str_mv (c) John Wiley & Sons, Inc, 2022
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) John Wiley & Sons, Inc, 2022
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,198674

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