Developmental stage as a possible factor affecting cytokinin content and cytokinin dehydrogenase activity in Pinus sylvestris

[EN] In the present study cytokinin dehydrogenase (CKX) activity was for the first time found in a conifer species, Pinus sylvestris. The activities were correlated with the endogenous cytokinin contents. Several enzyme substrates and two different electron acceptors were used to search for the enzy...

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Detalles Bibliográficos
Autores: Valdés, A.E., Galuszka, P., Fernández, B., Centeno Martín, María Luz, Frébort, I.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2007
País:España
Institución:Universidad de León
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/21009
Acceso en línea:https://hdl.handle.net/10612/21009
Access Level:acceso abierto
Palabra clave:Ingeniería agrícola
Cytokinin Metabolism
Conifers
Plant Ageing
2417.19 Fisiología Vegetal
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spelling Developmental stage as a possible factor affecting cytokinin content and cytokinin dehydrogenase activity in Pinus sylvestrisValdés, A.E.Galuszka, P.Fernández, B.Centeno Martín, María LuzFrébort, I.Ingeniería agrícolaCytokinin MetabolismConifersPlant Ageing2417.19 Fisiología Vegetal[EN] In the present study cytokinin dehydrogenase (CKX) activity was for the first time found in a conifer species, Pinus sylvestris. The activities were correlated with the endogenous cytokinin contents. Several enzyme substrates and two different electron acceptors were used to search for the enzyme activity in the extract from seeds, seedlings and plantlets. The highest specific activity was found in one-year-old plantlets with isopentenyladenine as the substrate and 2,6-dichlorophenolindophenol as the electron acceptor, at pH 8. An enhancement in the CKX specific activity corresponded to increasing contents of cytokinins, mainly isopentenyladenine and isopentenyladenosine, indicating that the enzyme activity is affected by the endogenous supply of cytokinins. CKX affinity for the ribosylated form of isopentenyladenine was dependent on the developmental stage, being higher in seeds than in seedlings, and not detectable in plantlets. The results are indicative of the presence of different isoenzymes throughout the development.SIThis research was carried out with financial support from the commission of the European Communities, Agriculture and Fisheries (FAIR) specific RTD program, CT96-1445 and from the Grant Agency of the Czech Republic 522/03/0979Springer NatureFisiologia VegetalEscuela de Ingeniería Agraria y Forestal2007info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://hdl.handle.net/10612/21009reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad de LeónIngléshttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/210092026-06-24T12:43:27Z
dc.title.none.fl_str_mv Developmental stage as a possible factor affecting cytokinin content and cytokinin dehydrogenase activity in Pinus sylvestris
title Developmental stage as a possible factor affecting cytokinin content and cytokinin dehydrogenase activity in Pinus sylvestris
spellingShingle Developmental stage as a possible factor affecting cytokinin content and cytokinin dehydrogenase activity in Pinus sylvestris
Valdés, A.E.
Ingeniería agrícola
Cytokinin Metabolism
Conifers
Plant Ageing
2417.19 Fisiología Vegetal
title_short Developmental stage as a possible factor affecting cytokinin content and cytokinin dehydrogenase activity in Pinus sylvestris
title_full Developmental stage as a possible factor affecting cytokinin content and cytokinin dehydrogenase activity in Pinus sylvestris
title_fullStr Developmental stage as a possible factor affecting cytokinin content and cytokinin dehydrogenase activity in Pinus sylvestris
title_full_unstemmed Developmental stage as a possible factor affecting cytokinin content and cytokinin dehydrogenase activity in Pinus sylvestris
title_sort Developmental stage as a possible factor affecting cytokinin content and cytokinin dehydrogenase activity in Pinus sylvestris
dc.creator.none.fl_str_mv Valdés, A.E.
Galuszka, P.
Fernández, B.
Centeno Martín, María Luz
Frébort, I.
author Valdés, A.E.
author_facet Valdés, A.E.
Galuszka, P.
Fernández, B.
Centeno Martín, María Luz
Frébort, I.
author_role author
author2 Galuszka, P.
Fernández, B.
Centeno Martín, María Luz
Frébort, I.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Fisiologia Vegetal
Escuela de Ingeniería Agraria y Forestal
dc.subject.none.fl_str_mv Ingeniería agrícola
Cytokinin Metabolism
Conifers
Plant Ageing
2417.19 Fisiología Vegetal
topic Ingeniería agrícola
Cytokinin Metabolism
Conifers
Plant Ageing
2417.19 Fisiología Vegetal
description [EN] In the present study cytokinin dehydrogenase (CKX) activity was for the first time found in a conifer species, Pinus sylvestris. The activities were correlated with the endogenous cytokinin contents. Several enzyme substrates and two different electron acceptors were used to search for the enzyme activity in the extract from seeds, seedlings and plantlets. The highest specific activity was found in one-year-old plantlets with isopentenyladenine as the substrate and 2,6-dichlorophenolindophenol as the electron acceptor, at pH 8. An enhancement in the CKX specific activity corresponded to increasing contents of cytokinins, mainly isopentenyladenine and isopentenyladenosine, indicating that the enzyme activity is affected by the endogenous supply of cytokinins. CKX affinity for the ribosylated form of isopentenyladenine was dependent on the developmental stage, being higher in seeds than in seedlings, and not detectable in plantlets. The results are indicative of the presence of different isoenzymes throughout the development.
publishDate 2007
dc.date.none.fl_str_mv 2007
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/10612/21009
url https://hdl.handle.net/10612/21009
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad de León
instname_str Universidad de León
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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