Id2 GABAergic interneurons comprise a neglected fourth major group of cortical inhibitory cells

Cortical GABAergic interneurons (INs) represent a diverse population of mainly locally projecting cells that provide specialized forms of inhibition to pyramidal neurons and other INs. Most recent work on INs has focused on subtypes distinguished by expression of Parvalbumin (PV), Somatostatin (SST)...

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Authors: Machold, Robert, Dellal, Shlomo, Valero, Manuel, Zurita, Hector, Kruglikov, Ilya, Meng, John Hongyu, Hanson, Jessica L., Hashikawa, Yoshiko, Schuman, Benjamin, Buzsáki, György, Rudy, Bernardo
Format: article
Status:Published version
Publication Date:2023
Country:España
Institution:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repository:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/60069
Online Access:http://hdl.handle.net/10230/60069
http://dx.doi.org/10.7554/eLife.85893
Access Level:Open access
Keyword:GABAergic Interneurons
Id2
Cortical inhibition
In vivo recordings
Intersectional genetics
Mouse
Neurogliaform cells
Neuroscience
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repository_id_str
spelling Id2 GABAergic interneurons comprise a neglected fourth major group of cortical inhibitory cellsMachold, RobertDellal, ShlomoValero, ManuelZurita, HectorKruglikov, IlyaMeng, John HongyuHanson, Jessica L.Hashikawa, YoshikoSchuman, BenjaminBuzsáki, GyörgyRudy, BernardoGABAergic InterneuronsId2Cortical inhibitionIn vivo recordingsIntersectional geneticsMouseNeurogliaform cellsNeuroscienceCortical GABAergic interneurons (INs) represent a diverse population of mainly locally projecting cells that provide specialized forms of inhibition to pyramidal neurons and other INs. Most recent work on INs has focused on subtypes distinguished by expression of Parvalbumin (PV), Somatostatin (SST), or Vasoactive Intestinal Peptide (VIP). However, a fourth group that includes neurogliaform cells (NGFCs) has been less well characterized due to a lack of genetic tools. Here, we show that these INs can be accessed experimentally using intersectional genetics with the gene Id2. We find that outside of layer 1 (L1), the majority of Id2 INs are NGFCs that express high levels of neuropeptide Y (NPY) and exhibit a late-spiking firing pattern, with extensive local connectivity. While much sparser, non-NGFC Id2 INs had more variable properties, with most cells corresponding to a diverse group of INs that strongly expresses the neuropeptide CCK. In vivo, using silicon probe recordings, we observed several distinguishing aspects of NGFC activity, including a strong rebound in activity immediately following the cortical down state during NREM sleep. Our study provides insights into IN diversity and NGFC distribution and properties, and outlines an intersectional genetics approach for further study of this underappreciated group of INs.eLife202420242023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/60069http://dx.doi.org/10.7554/eLife.85893reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésElife. 2023 Sep 4;12:e85893© 2023, Machold et al. This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use and redistribution provided that the original author and source are credited.http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10230/600692026-05-29T05:05:01Z
dc.title.none.fl_str_mv Id2 GABAergic interneurons comprise a neglected fourth major group of cortical inhibitory cells
title Id2 GABAergic interneurons comprise a neglected fourth major group of cortical inhibitory cells
spellingShingle Id2 GABAergic interneurons comprise a neglected fourth major group of cortical inhibitory cells
Machold, Robert
GABAergic Interneurons
Id2
Cortical inhibition
In vivo recordings
Intersectional genetics
Mouse
Neurogliaform cells
Neuroscience
title_short Id2 GABAergic interneurons comprise a neglected fourth major group of cortical inhibitory cells
title_full Id2 GABAergic interneurons comprise a neglected fourth major group of cortical inhibitory cells
title_fullStr Id2 GABAergic interneurons comprise a neglected fourth major group of cortical inhibitory cells
title_full_unstemmed Id2 GABAergic interneurons comprise a neglected fourth major group of cortical inhibitory cells
title_sort Id2 GABAergic interneurons comprise a neglected fourth major group of cortical inhibitory cells
dc.creator.none.fl_str_mv Machold, Robert
Dellal, Shlomo
Valero, Manuel
Zurita, Hector
Kruglikov, Ilya
Meng, John Hongyu
Hanson, Jessica L.
Hashikawa, Yoshiko
Schuman, Benjamin
Buzsáki, György
Rudy, Bernardo
author Machold, Robert
author_facet Machold, Robert
Dellal, Shlomo
Valero, Manuel
Zurita, Hector
Kruglikov, Ilya
Meng, John Hongyu
Hanson, Jessica L.
Hashikawa, Yoshiko
Schuman, Benjamin
Buzsáki, György
Rudy, Bernardo
author_role author
author2 Dellal, Shlomo
Valero, Manuel
Zurita, Hector
Kruglikov, Ilya
Meng, John Hongyu
Hanson, Jessica L.
Hashikawa, Yoshiko
Schuman, Benjamin
Buzsáki, György
Rudy, Bernardo
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv GABAergic Interneurons
Id2
Cortical inhibition
In vivo recordings
Intersectional genetics
Mouse
Neurogliaform cells
Neuroscience
topic GABAergic Interneurons
Id2
Cortical inhibition
In vivo recordings
Intersectional genetics
Mouse
Neurogliaform cells
Neuroscience
description Cortical GABAergic interneurons (INs) represent a diverse population of mainly locally projecting cells that provide specialized forms of inhibition to pyramidal neurons and other INs. Most recent work on INs has focused on subtypes distinguished by expression of Parvalbumin (PV), Somatostatin (SST), or Vasoactive Intestinal Peptide (VIP). However, a fourth group that includes neurogliaform cells (NGFCs) has been less well characterized due to a lack of genetic tools. Here, we show that these INs can be accessed experimentally using intersectional genetics with the gene Id2. We find that outside of layer 1 (L1), the majority of Id2 INs are NGFCs that express high levels of neuropeptide Y (NPY) and exhibit a late-spiking firing pattern, with extensive local connectivity. While much sparser, non-NGFC Id2 INs had more variable properties, with most cells corresponding to a diverse group of INs that strongly expresses the neuropeptide CCK. In vivo, using silicon probe recordings, we observed several distinguishing aspects of NGFC activity, including a strong rebound in activity immediately following the cortical down state during NREM sleep. Our study provides insights into IN diversity and NGFC distribution and properties, and outlines an intersectional genetics approach for further study of this underappreciated group of INs.
publishDate 2023
dc.date.none.fl_str_mv 2023
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/60069
http://dx.doi.org/10.7554/eLife.85893
url http://hdl.handle.net/10230/60069
http://dx.doi.org/10.7554/eLife.85893
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Elife. 2023 Sep 4;12:e85893
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv eLife
publisher.none.fl_str_mv eLife
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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