Changes during epicotyl growth of an autolysis-related β-galactosidase from the cell wall of Cicer arietinum

During the growth of Cicer arietinum epicotyls an increase in specific cell wall beta-glucosidase and beta-galactosidase activities were observed. In contrast, the amount of cell wall proteins extracted with 3 M LiCI decreased between the third and the fourth day of germination, remaining constant u...

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Detalles Bibliográficos
Autores: Dopico, Berta, Nicolás Rodrigo, Gregorio, Labrador, Emilia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:1990
País:España
Institución:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/157392
Acceso en línea:http://hdl.handle.net/10366/157392
Access Level:acceso embargado
Palabra clave:Autolysis
Cicer arietinum
Epicotyls
Beta-galactosidase
Glucosidases, growth
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spelling Changes during epicotyl growth of an autolysis-related β-galactosidase from the cell wall of Cicer arietinumDopico, BertaNicolás Rodrigo, GregorioLabrador, EmiliaAutolysisCicer arietinumEpicotylsBeta-galactosidaseGlucosidases, growthDuring the growth of Cicer arietinum epicotyls an increase in specific cell wall beta-glucosidase and beta-galactosidase activities were observed. In contrast, the amount of cell wall proteins extracted with 3 M LiCI decreased between the third and the fourth day of germination, remaining constant up to the seventh day. Partial purification of the cell wall protein extracts from different days by SP­-Sephadex chromatography showed that one fraction increased both in the amount of protein and beta-galactosidase activity throughout the growth of epicotyls. This protein fraction, which is the third beta-galactosidase (betaIII) eluted from a SP-Sephadex chromatography, has been previously characterized by us as the main enzyme involved in the autolytic process.This work was supported by a grant from the Dirección General de Investigación Científica y Técnica, Spain No. PB87-0024.info202420241990info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10366/157392reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésPB87-0024Attribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/embargoedAccessoai:gredos.usal.es:10366/1573922026-06-07T06:28:51Z
dc.title.none.fl_str_mv Changes during epicotyl growth of an autolysis-related β-galactosidase from the cell wall of Cicer arietinum
title Changes during epicotyl growth of an autolysis-related β-galactosidase from the cell wall of Cicer arietinum
spellingShingle Changes during epicotyl growth of an autolysis-related β-galactosidase from the cell wall of Cicer arietinum
Dopico, Berta
Autolysis
Cicer arietinum
Epicotyls
Beta-galactosidase
Glucosidases, growth
title_short Changes during epicotyl growth of an autolysis-related β-galactosidase from the cell wall of Cicer arietinum
title_full Changes during epicotyl growth of an autolysis-related β-galactosidase from the cell wall of Cicer arietinum
title_fullStr Changes during epicotyl growth of an autolysis-related β-galactosidase from the cell wall of Cicer arietinum
title_full_unstemmed Changes during epicotyl growth of an autolysis-related β-galactosidase from the cell wall of Cicer arietinum
title_sort Changes during epicotyl growth of an autolysis-related β-galactosidase from the cell wall of Cicer arietinum
dc.creator.none.fl_str_mv Dopico, Berta
Nicolás Rodrigo, Gregorio
Labrador, Emilia
author Dopico, Berta
author_facet Dopico, Berta
Nicolás Rodrigo, Gregorio
Labrador, Emilia
author_role author
author2 Nicolás Rodrigo, Gregorio
Labrador, Emilia
author2_role author
author
dc.subject.none.fl_str_mv Autolysis
Cicer arietinum
Epicotyls
Beta-galactosidase
Glucosidases, growth
topic Autolysis
Cicer arietinum
Epicotyls
Beta-galactosidase
Glucosidases, growth
description During the growth of Cicer arietinum epicotyls an increase in specific cell wall beta-glucosidase and beta-galactosidase activities were observed. In contrast, the amount of cell wall proteins extracted with 3 M LiCI decreased between the third and the fourth day of germination, remaining constant up to the seventh day. Partial purification of the cell wall protein extracts from different days by SP­-Sephadex chromatography showed that one fraction increased both in the amount of protein and beta-galactosidase activity throughout the growth of epicotyls. This protein fraction, which is the third beta-galactosidase (betaIII) eluted from a SP-Sephadex chromatography, has been previously characterized by us as the main enzyme involved in the autolytic process.
publishDate 1990
dc.date.none.fl_str_mv 1990
2024
2024
info
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10366/157392
url http://hdl.handle.net/10366/157392
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv PB87-0024
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/embargoedAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv embargoedAccess
dc.source.none.fl_str_mv reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca
instname:Universidad de Salamanca (USAL)
instname_str Universidad de Salamanca (USAL)
reponame_str GREDOS. Repositorio Institucional de la Universidad de Salamanca
collection GREDOS. Repositorio Institucional de la Universidad de Salamanca
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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