Hyaluronic acid-based bioink improves the differentiation and network formation of neural progenitor cells

Introduction: Three-dimensional (3D) bioprinting is a promising technique for the development of neuronal in vitro models because it controls the deposition of materials and cells. Finding a biomaterial that supports neural differentiation in vitro while ensuring compatibility with the technique of...

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Autores: Pereira, Inês Sousa, López Martínez, María José, Villasante, Aranzazu, Introna, Clelia, Tornero, Daniel, Canals i Coll, Josep M., Samitier i Martí, Josep
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/195645
Acceso en línea:https://hdl.handle.net/2445/195645
Access Level:acceso abierto
Palabra clave:Materials biomèdics
Impressió 3D
Àcid hialurònic
Diferenciació de productes
Regeneració del sistema nerviós
Biomedical materials
Three-dimensional printing
Hyaluronic acid
Product differentiation
Nervous system regeneration
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spelling Hyaluronic acid-based bioink improves the differentiation and network formation of neural progenitor cellsPereira, Inês SousaLópez Martínez, María JoséVillasante, AranzazuIntrona, CleliaTornero, DanielCanals i Coll, Josep M.Samitier i Martí, JosepMaterials biomèdicsImpressió 3DÀcid hialurònicDiferenciació de productesRegeneració del sistema nerviósBiomedical materialsThree-dimensional printingHyaluronic acidProduct differentiationNervous system regenerationIntroduction: Three-dimensional (3D) bioprinting is a promising technique for the development of neuronal in vitro models because it controls the deposition of materials and cells. Finding a biomaterial that supports neural differentiation in vitro while ensuring compatibility with the technique of 3D bioprinting of a self-standing construct is a challenge. Methods: In this study, gelatin methacryloyl (GelMA), methacrylated alginate (AlgMA), and hyaluronic acid (HA) were examined by exploiting their biocompatibility and tunable mechanical properties to resemble the extracellular matrix (ECM) and to create a suitable material for printing neural progenitor cells (NPCs), supporting their long-term differentiation. NPCs were printed and differentiated for up to 15 days, and cell viability and neuronal differentiation markers were assessed throughout the culture. Results and Discussion: This composite biomaterial presented the desired physical properties to mimic the ECM of the brain with high water intake, low stiffness, and slow degradation while allowing the printing of defined structures. The viability rates were maintained at approximately 80% at all time points. However, the levels of β-III tubulin marker increased over time, demonstrating the compatibility of this biomaterial with neuronal cell culture and differentiation. Furthermore, these cells showed increased maturation with corresponding functional properties, which was also demonstrated by the formation of a neuronal network that was observed by recording spontaneous activity via Ca2+ imaging.Frontiers Media2023202320232023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion15 p.application/pdfapplication/pdfhttps://hdl.handle.net/2445/195645Articles publicats en revistes (Biomedicina)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.3389/fbioe.2023.1110547Frontiers In Bioengineering And Biotechnology, 2023, vol. 11, p. 1110547https://doi.org/10.3389/fbioe.2023.1110547cc-by (c) Pereira, Inês et al., 2023https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1956452026-05-29T05:05:01Z
dc.title.none.fl_str_mv Hyaluronic acid-based bioink improves the differentiation and network formation of neural progenitor cells
title Hyaluronic acid-based bioink improves the differentiation and network formation of neural progenitor cells
spellingShingle Hyaluronic acid-based bioink improves the differentiation and network formation of neural progenitor cells
Pereira, Inês Sousa
Materials biomèdics
Impressió 3D
Àcid hialurònic
Diferenciació de productes
Regeneració del sistema nerviós
Biomedical materials
Three-dimensional printing
Hyaluronic acid
Product differentiation
Nervous system regeneration
title_short Hyaluronic acid-based bioink improves the differentiation and network formation of neural progenitor cells
title_full Hyaluronic acid-based bioink improves the differentiation and network formation of neural progenitor cells
title_fullStr Hyaluronic acid-based bioink improves the differentiation and network formation of neural progenitor cells
title_full_unstemmed Hyaluronic acid-based bioink improves the differentiation and network formation of neural progenitor cells
title_sort Hyaluronic acid-based bioink improves the differentiation and network formation of neural progenitor cells
dc.creator.none.fl_str_mv Pereira, Inês Sousa
López Martínez, María José
Villasante, Aranzazu
Introna, Clelia
Tornero, Daniel
Canals i Coll, Josep M.
Samitier i Martí, Josep
author Pereira, Inês Sousa
author_facet Pereira, Inês Sousa
López Martínez, María José
Villasante, Aranzazu
Introna, Clelia
Tornero, Daniel
Canals i Coll, Josep M.
Samitier i Martí, Josep
author_role author
author2 López Martínez, María José
Villasante, Aranzazu
Introna, Clelia
Tornero, Daniel
Canals i Coll, Josep M.
Samitier i Martí, Josep
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Materials biomèdics
Impressió 3D
Àcid hialurònic
Diferenciació de productes
Regeneració del sistema nerviós
Biomedical materials
Three-dimensional printing
Hyaluronic acid
Product differentiation
Nervous system regeneration
topic Materials biomèdics
Impressió 3D
Àcid hialurònic
Diferenciació de productes
Regeneració del sistema nerviós
Biomedical materials
Three-dimensional printing
Hyaluronic acid
Product differentiation
Nervous system regeneration
description Introduction: Three-dimensional (3D) bioprinting is a promising technique for the development of neuronal in vitro models because it controls the deposition of materials and cells. Finding a biomaterial that supports neural differentiation in vitro while ensuring compatibility with the technique of 3D bioprinting of a self-standing construct is a challenge. Methods: In this study, gelatin methacryloyl (GelMA), methacrylated alginate (AlgMA), and hyaluronic acid (HA) were examined by exploiting their biocompatibility and tunable mechanical properties to resemble the extracellular matrix (ECM) and to create a suitable material for printing neural progenitor cells (NPCs), supporting their long-term differentiation. NPCs were printed and differentiated for up to 15 days, and cell viability and neuronal differentiation markers were assessed throughout the culture. Results and Discussion: This composite biomaterial presented the desired physical properties to mimic the ECM of the brain with high water intake, low stiffness, and slow degradation while allowing the printing of defined structures. The viability rates were maintained at approximately 80% at all time points. However, the levels of β-III tubulin marker increased over time, demonstrating the compatibility of this biomaterial with neuronal cell culture and differentiation. Furthermore, these cells showed increased maturation with corresponding functional properties, which was also demonstrated by the formation of a neuronal network that was observed by recording spontaneous activity via Ca2+ imaging.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/195645
url https://hdl.handle.net/2445/195645
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3389/fbioe.2023.1110547
Frontiers In Bioengineering And Biotechnology, 2023, vol. 11, p. 1110547
https://doi.org/10.3389/fbioe.2023.1110547
dc.rights.none.fl_str_mv cc-by (c) Pereira, Inês et al., 2023
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Pereira, Inês et al., 2023
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 15 p.
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv Articles publicats en revistes (Biomedicina)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869420543855296512
score 15.81155

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