Antitumor magnetic hyperthermia induced by RGD-functionalized Fe3O4 nanoparticles, in an experimental model of colorectal liver metastases

This work reports important advances in the study of magnetic nanoparticles (MNPs) related to their application in different research fields such as magnetic hyperthermia. Nanotherapy based on targeted nanoparticles could become an attractive alternative to conventional oncologic treatments as it al...

Full description

Bibliographic Details
Authors: Arriortua, Oihane, Garaio, Eneko, Herrero de la Parte, Borja, Insausti Peña, María Teresa, Lezama Diago, Luis María, Plazaola Muguruza, Fernando, García Martínez, José Ángel, Aizpurua Iparraguirre, Jesús María, Sagartzazu Aizpurua, Maialen, Irazola Duñabeitia, Mireia, Etxebarria Loizate, Nestor, García-Alonso Montoya, Ignacio, Saiz López, Alberto, Echevarría Uraga, Jose Javier
Format: article
Publication Date:2016
Country:España
Institution:Universidad del País Vasco
Repository:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/64458
Online Access:http://hdl.handle.net/10810/64458
Access Level:Open access
Keyword:magnetite nanoparticles
magnetic hyperthermia
RGD functionalization
tumor targeting
id ES_d4607f4429ba8bc5831a1bbdbce14efb
oai_identifier_str oai:addi.ehu.eus:10810/64458
network_acronym_str ES
network_name_str España
repository_id_str
spelling Antitumor magnetic hyperthermia induced by RGD-functionalized Fe3O4 nanoparticles, in an experimental model of colorectal liver metastasesArriortua, OihaneGaraio, EnekoHerrero de la Parte, BorjaInsausti Peña, María TeresaLezama Diago, Luis MaríaPlazaola Muguruza, FernandoGarcía Martínez, José ÁngelAizpurua Iparraguirre, Jesús MaríaSagartzazu Aizpurua, MaialenIrazola Duñabeitia, MireiaEtxebarria Loizate, NestorGarcía-Alonso Montoya, IgnacioSaiz López, AlbertoEchevarría Uraga, Jose Javiermagnetite nanoparticlesmagnetic hyperthermiaRGD functionalizationtumor targetingThis work reports important advances in the study of magnetic nanoparticles (MNPs) related to their application in different research fields such as magnetic hyperthermia. Nanotherapy based on targeted nanoparticles could become an attractive alternative to conventional oncologic treatments as it allows a local heating in tumoral surroundings without damage to healthy tissue. RGD-peptide-conjugated MNPs have been designed to specifically target αVβ3 receptor-expressing cancer cells, being bound the RGD peptides by “click chemistry” due to its selectivity and applicability. The thermal decomposition of iron metallo-organic precursors yield homogeneous Fe3O4 nanoparticles that have been properly functionalized with RGD peptides, and the preparation of magnetic fluids has been achieved. The nanoparticles were characterized by transmission electron microscopy (TEM), vibrating sample magnetometry (VSM), electron magnetic resonance (EMR) spectroscopy and magnetic hyperthermia. The nanoparticles present superparamagnetic behavior with very high magnetization values, which yield hyperthermia values above 500 W/g for magnetic fluids. These fluids have been administrated to rats, but instead of injecting MNP fluid directly into liver tumors, intravascular administration of MNPs in animals with induced colorectal tumors has been performed. Afterwards the animals were exposed to an alternating magnetic field in order to achieve hyperthermia. The evolution of an in vivo model has been described, resulting in a significant reduction in tumor viability.This work was supported by institutional funding from the Ministerio de Economía y Competitividad and Basque Government under Projects MAT2013-41128-R, FEDER and GIC-IT-570-13.Beilstein-Institut202420242016info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/64458reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoInglésinfo:eu-repo/grantAgreement/MINECO/MAT2013-41128-R/https://doi.org/10.3762/bjnano.7.147info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/es/This is an Open Access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.oai:addi.ehu.eus:10810/644582026-06-18T09:23:17Z
dc.title.none.fl_str_mv Antitumor magnetic hyperthermia induced by RGD-functionalized Fe3O4 nanoparticles, in an experimental model of colorectal liver metastases
title Antitumor magnetic hyperthermia induced by RGD-functionalized Fe3O4 nanoparticles, in an experimental model of colorectal liver metastases
spellingShingle Antitumor magnetic hyperthermia induced by RGD-functionalized Fe3O4 nanoparticles, in an experimental model of colorectal liver metastases
Arriortua, Oihane
magnetite nanoparticles
magnetic hyperthermia
RGD functionalization
tumor targeting
title_short Antitumor magnetic hyperthermia induced by RGD-functionalized Fe3O4 nanoparticles, in an experimental model of colorectal liver metastases
title_full Antitumor magnetic hyperthermia induced by RGD-functionalized Fe3O4 nanoparticles, in an experimental model of colorectal liver metastases
title_fullStr Antitumor magnetic hyperthermia induced by RGD-functionalized Fe3O4 nanoparticles, in an experimental model of colorectal liver metastases
title_full_unstemmed Antitumor magnetic hyperthermia induced by RGD-functionalized Fe3O4 nanoparticles, in an experimental model of colorectal liver metastases
title_sort Antitumor magnetic hyperthermia induced by RGD-functionalized Fe3O4 nanoparticles, in an experimental model of colorectal liver metastases
dc.creator.none.fl_str_mv Arriortua, Oihane
Garaio, Eneko
Herrero de la Parte, Borja
Insausti Peña, María Teresa
Lezama Diago, Luis María
Plazaola Muguruza, Fernando
García Martínez, José Ángel
Aizpurua Iparraguirre, Jesús María
Sagartzazu Aizpurua, Maialen
Irazola Duñabeitia, Mireia
Etxebarria Loizate, Nestor
García-Alonso Montoya, Ignacio
Saiz López, Alberto
Echevarría Uraga, Jose Javier
author Arriortua, Oihane
author_facet Arriortua, Oihane
Garaio, Eneko
Herrero de la Parte, Borja
Insausti Peña, María Teresa
Lezama Diago, Luis María
Plazaola Muguruza, Fernando
García Martínez, José Ángel
Aizpurua Iparraguirre, Jesús María
Sagartzazu Aizpurua, Maialen
Irazola Duñabeitia, Mireia
Etxebarria Loizate, Nestor
García-Alonso Montoya, Ignacio
Saiz López, Alberto
Echevarría Uraga, Jose Javier
author_role author
author2 Garaio, Eneko
Herrero de la Parte, Borja
Insausti Peña, María Teresa
Lezama Diago, Luis María
Plazaola Muguruza, Fernando
García Martínez, José Ángel
Aizpurua Iparraguirre, Jesús María
Sagartzazu Aizpurua, Maialen
Irazola Duñabeitia, Mireia
Etxebarria Loizate, Nestor
García-Alonso Montoya, Ignacio
Saiz López, Alberto
Echevarría Uraga, Jose Javier
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv magnetite nanoparticles
magnetic hyperthermia
RGD functionalization
tumor targeting
topic magnetite nanoparticles
magnetic hyperthermia
RGD functionalization
tumor targeting
description This work reports important advances in the study of magnetic nanoparticles (MNPs) related to their application in different research fields such as magnetic hyperthermia. Nanotherapy based on targeted nanoparticles could become an attractive alternative to conventional oncologic treatments as it allows a local heating in tumoral surroundings without damage to healthy tissue. RGD-peptide-conjugated MNPs have been designed to specifically target αVβ3 receptor-expressing cancer cells, being bound the RGD peptides by “click chemistry” due to its selectivity and applicability. The thermal decomposition of iron metallo-organic precursors yield homogeneous Fe3O4 nanoparticles that have been properly functionalized with RGD peptides, and the preparation of magnetic fluids has been achieved. The nanoparticles were characterized by transmission electron microscopy (TEM), vibrating sample magnetometry (VSM), electron magnetic resonance (EMR) spectroscopy and magnetic hyperthermia. The nanoparticles present superparamagnetic behavior with very high magnetization values, which yield hyperthermia values above 500 W/g for magnetic fluids. These fluids have been administrated to rats, but instead of injecting MNP fluid directly into liver tumors, intravascular administration of MNPs in animals with induced colorectal tumors has been performed. Afterwards the animals were exposed to an alternating magnetic field in order to achieve hyperthermia. The evolution of an in vivo model has been described, resulting in a significant reduction in tumor viability.
publishDate 2016
dc.date.none.fl_str_mv 2016
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/64458
url http://hdl.handle.net/10810/64458
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO/MAT2013-41128-R/
https://doi.org/10.3762/bjnano.7.147
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/es/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Beilstein-Institut
publisher.none.fl_str_mv Beilstein-Institut
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869420541559963648
score 15,301603

Similar Items