Synthetic lethality between the cohesin subunits STAG1 and STAG2 in diverse cancer contexts

Recent genome analyses have identified recurrent mutations in the cohesin complex in a wide range of human cancers. Here we demonstrate that the most frequently mutated subunit of the cohesin complex, STAG2, displays a strong synthetic lethal interaction with its paralog STAG1. Mechanistically, STAG...

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Detalles Bibliográficos
Autores: van der Lelij, Petra, Lieb, Simone, Jude, Julian, Wutz, Gordana, Santos, Catarina P., Falkenberg, Katrina, Schlattl, Andreas, Ban, Jozef, Schwentner, Raphaela, Hoffmann, Thomas, Kovar, Heinrich, Real, Francisco X., Waldman, Todd, Pearson, Mark A., Kraut, Norbert, Peters, Jan-Michael, Zuber, Johannes, Petronczki, Mark
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/33224
Acceso en línea:http://hdl.handle.net/10230/33224
http://dx.doi.org/10.7554/eLife.26980
Access Level:acceso abierto
Palabra clave:Cancer biology
Cell biology
Cell division
Chromosomes
Cohesin
Genetic interaction
Human
Mitosis
Synthetic lethality
Descripción
Sumario:Recent genome analyses have identified recurrent mutations in the cohesin complex in a wide range of human cancers. Here we demonstrate that the most frequently mutated subunit of the cohesin complex, STAG2, displays a strong synthetic lethal interaction with its paralog STAG1. Mechanistically, STAG1 loss abrogates sister chromatid cohesion in STAG2 mutated but not in wild-type cells leading to mitotic catastrophe, defective cell division and apoptosis. STAG1 inactivation inhibits the proliferation of STAG2 mutated but not wild-type bladder cancer and Ewing sarcoma cell lines. Restoration of STAG2 expression in a mutated bladder cancer model alleviates the dependency on STAG1. Thus, STAG1 and STAG2 support sister chromatid cohesion to redundantly ensure cell survival. STAG1 represents a vulnerability of cancer cells carrying mutations in the major emerging tumor suppressor STAG2 across different cancer contexts. Exploiting synthetic lethal interactions to target recurrent cohesin mutations in cancer, e.g. by inhibiting STAG1, holds the promise for the development of selective therapeutics.

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