Placental thrombosis in acute phase abortions during experimental Toxoplasma gondii infection in sheep

After oral administration of ewes during mid gestation with 2000 freshly prepared sporulated oocysts of T. gondii isolate M4, abortions occurred between days 7 and 11 in 91.6% of pregnant and infected ewes. Afterwards, a further infection was carried out at late gestation in another group of sheep w...

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Autores: Castaño, P., Fuertes Franco, Miguel, Fernández Fernández, Miguel, Ferreras, Mª del Carmen, González Lanza, Camino, Pérez Pérez, Valentín, Benavides, Julio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/92296
Acceso en línea:http://hdl.handle.net/10261/92296
Access Level:acceso abierto
Palabra clave:Acquired-immunodeficiency-syndrome
Ovine toxoplasmosis
Pregnant sheep
Oocysts
Vaccine
Transmission
Challenge
Cells
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spelling Placental thrombosis in acute phase abortions during experimental Toxoplasma gondii infection in sheepCastaño, P.Fuertes Franco, MiguelFernández Fernández, MiguelFerreras, Mª del CarmenGonzález Lanza, CaminoPérez Pérez, ValentínBenavides, JulioAcquired-immunodeficiency-syndromeOvine toxoplasmosisPregnant sheepOocystsVaccineTransmissionChallengeCellsAfter oral administration of ewes during mid gestation with 2000 freshly prepared sporulated oocysts of T. gondii isolate M4, abortions occurred between days 7 and 11 in 91.6% of pregnant and infected ewes. Afterwards, a further infection was carried out at late gestation in another group of sheep with 500 sporulated oocysts. Abortions happened again between days 9 and 11 post infection (pi) in 58.3% of the infected ewes. Classically, abortions in natural and experimental ovine toxoplasmosis usually occur one month after infection. Few experimental studies have reported the so-called acute phase abortions as early as 7 to 14 days after oral inoculation of oocysts, and pyrexia was proposed to be responsible for abortion, although the underline mechanism was not elucidated. In the present study, all placentas analysed from ewes suffering acute phase abortions showed infarcts and thrombosis in the caruncullar villi of the placentomes and ischemic lesions (periventricular leukomalacia) in the brain of some foetuses. The parasite was identified by PCR in samples from some placentomes of only one sheep, and no antigen was detected by immunohistochemical labelling. These findings suggest that the vascular lesions found in the placenta, and the consequent hypoxic damage to the foetus, could be associated to the occurrence of acute phase abortions. Although the pathogenesis of these lesions remains to be determined, the infectious dose or virulence of the isolate may play a role in their development.This work was supported by grants AGL2011-30205, of the Spanish Ministry of Science and Innovation, and LE253U13, from the Junta de Castilla y Leon, Spain. J. Benavides is supported by CSIC through the JAE-Doc program, financed in part by European Social Fund (ESF).Peer ReviewedBioMed CentralMinisterio de Ciencia e Innovación (España)Junta de Castilla y LeónConsejo Superior de Investigaciones Científicas (España)European CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2014201420142014info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/92296reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1186/1297-9716-45-9Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/922962026-05-22T06:33:51Z
dc.title.none.fl_str_mv Placental thrombosis in acute phase abortions during experimental Toxoplasma gondii infection in sheep
title Placental thrombosis in acute phase abortions during experimental Toxoplasma gondii infection in sheep
spellingShingle Placental thrombosis in acute phase abortions during experimental Toxoplasma gondii infection in sheep
Castaño, P.
Acquired-immunodeficiency-syndrome
Ovine toxoplasmosis
Pregnant sheep
Oocysts
Vaccine
Transmission
Challenge
Cells
title_short Placental thrombosis in acute phase abortions during experimental Toxoplasma gondii infection in sheep
title_full Placental thrombosis in acute phase abortions during experimental Toxoplasma gondii infection in sheep
title_fullStr Placental thrombosis in acute phase abortions during experimental Toxoplasma gondii infection in sheep
title_full_unstemmed Placental thrombosis in acute phase abortions during experimental Toxoplasma gondii infection in sheep
title_sort Placental thrombosis in acute phase abortions during experimental Toxoplasma gondii infection in sheep
dc.creator.none.fl_str_mv Castaño, P.
Fuertes Franco, Miguel
Fernández Fernández, Miguel
Ferreras, Mª del Carmen
González Lanza, Camino
Pérez Pérez, Valentín
Benavides, Julio
author Castaño, P.
author_facet Castaño, P.
Fuertes Franco, Miguel
Fernández Fernández, Miguel
Ferreras, Mª del Carmen
González Lanza, Camino
Pérez Pérez, Valentín
Benavides, Julio
author_role author
author2 Fuertes Franco, Miguel
Fernández Fernández, Miguel
Ferreras, Mª del Carmen
González Lanza, Camino
Pérez Pérez, Valentín
Benavides, Julio
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Junta de Castilla y León
Consejo Superior de Investigaciones Científicas (España)
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Acquired-immunodeficiency-syndrome
Ovine toxoplasmosis
Pregnant sheep
Oocysts
Vaccine
Transmission
Challenge
Cells
topic Acquired-immunodeficiency-syndrome
Ovine toxoplasmosis
Pregnant sheep
Oocysts
Vaccine
Transmission
Challenge
Cells
description After oral administration of ewes during mid gestation with 2000 freshly prepared sporulated oocysts of T. gondii isolate M4, abortions occurred between days 7 and 11 in 91.6% of pregnant and infected ewes. Afterwards, a further infection was carried out at late gestation in another group of sheep with 500 sporulated oocysts. Abortions happened again between days 9 and 11 post infection (pi) in 58.3% of the infected ewes. Classically, abortions in natural and experimental ovine toxoplasmosis usually occur one month after infection. Few experimental studies have reported the so-called acute phase abortions as early as 7 to 14 days after oral inoculation of oocysts, and pyrexia was proposed to be responsible for abortion, although the underline mechanism was not elucidated. In the present study, all placentas analysed from ewes suffering acute phase abortions showed infarcts and thrombosis in the caruncullar villi of the placentomes and ischemic lesions (periventricular leukomalacia) in the brain of some foetuses. The parasite was identified by PCR in samples from some placentomes of only one sheep, and no antigen was detected by immunohistochemical labelling. These findings suggest that the vascular lesions found in the placenta, and the consequent hypoxic damage to the foetus, could be associated to the occurrence of acute phase abortions. Although the pathogenesis of these lesions remains to be determined, the infectious dose or virulence of the isolate may play a role in their development.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014
2014
2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/92296
url http://hdl.handle.net/10261/92296
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1186/1297-9716-45-9
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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