Quantitative imaging in 3D microscopy with partially coherent illumination
The main research objective of this work is to develop and implement a fast and high resolution technique for reconstructing the 3D refractive index (RI) of weakly absorbing samples. This technique is based on the generalization of optical diffraction tomography (ODT), developed for coherent sample...
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| Tipo de documento: | tese |
| Data de publicação: | 2021 |
| País: | España |
| Recursos: | Universidad Complutense de Madrid (UCM) |
| Repositório: | Docta Complutense |
| Idioma: | inglês |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/11607 |
| Acesso em linha: | https://hdl.handle.net/20.500.14352/11607 |
| Access Level: | Acceso aberto |
| Palavra-chave: | 535(043.2) 3D microscopy Microscopia 3D Óptica (Física) 2209.19 Óptica Física |
| Resumo: | The main research objective of this work is to develop and implement a fast and high resolution technique for reconstructing the 3D refractive index (RI) of weakly absorbing samples. This technique is based on the generalization of optical diffraction tomography (ODT), developed for coherent sample illumination (C-ODT), to the partially coherent case (PC-ODT). In contrast to C-ODT, which involves a specially designed holographic microscope, the proposed PC-ODT technique is implemented in conventional wide-field microscope. The incorporation of the required refocusing module is easy and can be done by a final user unfamiliar with optics (i.e. clinicians and biologists). The low coherent illumination provided by high numerical aperture condenser results in high resolution and reduced speckle noise typical for laser sources used in C-ODT... |
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