Advances in structure elucidation of low molecular weight carbohydrates by liquid chromatography-multiple-stage mass spectrometry analysis

Oligosaccharides are gaining importance because of their beneficial properties in human health. They normally appear in natural and synthetic products as complex mixtures of different monomeric units, glycosidic linkages and degrees of polymerization, being disaccharides and trisaccharides usually t...

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Autores: Martín-Ortiz, Andrea, Carrero-Carralero, Cipriano, Hernández-Hernández, Oswaldo, Lebrón-Aguilar, Rosa, Moreno, F. Javier, Sanz, M. Luz, Ruiz-Matute, Ana I.
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/204495
Acceso en línea:http://hdl.handle.net/10261/204495
Access Level:acceso abierto
Palabra clave:Disaccharides
Trisaccharides
Liquid chromatography-multiple-stage mass spectrometry
Glycosidic linkage
Monomeric units
Diagnostic fragment ions
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spelling Advances in structure elucidation of low molecular weight carbohydrates by liquid chromatography-multiple-stage mass spectrometry analysisMartín-Ortiz, AndreaCarrero-Carralero, CiprianoHernández-Hernández, OswaldoLebrón-Aguilar, RosaMoreno, F. JavierSanz, M. LuzRuiz-Matute, Ana I.DisaccharidesTrisaccharidesLiquid chromatography-multiple-stage mass spectrometryGlycosidic linkageMonomeric unitsDiagnostic fragment ionsOligosaccharides are gaining importance because of their beneficial properties in human health. They normally appear in natural and synthetic products as complex mixtures of different monomeric units, glycosidic linkages and degrees of polymerization, being disaccharides and trisaccharides usually the most abundant ones. Although liquid chromatography-mass spectrometry is a useful technique for oligosaccharides analysis, the similarity of their structures makes difficult their characterization. Moreover, there is still scarce information about the relationship between carbohydrate chemical structure, mass spectra and chromatographic data. Then, in this work, chromatographic parameters for 23 disaccharides with different linkages and monomeric units (glucose, galactose, mannose and fructose) were determined using porous graphitized and hydrophilic interaction liquid chromatography columns. Moreover, diagnostic ions of these disaccharides obtained by tandem mass spectra (MS) were established by stepwise linear discriminant analysis. The relationship between carbohydrate chemical structure and their chromatographic retention data and characteristic ions obtained by multiple-stage mass spectrometry (MS) was successful in establishing some specific criteria that allowed the characterization of trisaccharides with different structural features.This work was supported by Ministerio de Economía, Industria y Competitividad (MINECO) of Spain (projects AGL2016-80475-R and AGL2017-84614-C2-1-R) and by the Comunidad of Madrid and European funding from FSE and FEDER programs (project S2018/BAA-4393, AVANSECAL-II-CM).ElsevierMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)European CommissionMinisterio de Economía, Industria y Competitividad (España)Comunidad de MadridMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2020202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/204495reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016-80475-RS2018/BAA-4393/AVANSECAL-II-CMinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/AGL2017-84614-C2-1-RAGL2017-84614-C2-1-R /AEI/10.13039/501100011033http://dx.doi.org/10.1016/j.chroma.2019.460664Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2044952026-05-22T06:33:51Z
dc.title.none.fl_str_mv Advances in structure elucidation of low molecular weight carbohydrates by liquid chromatography-multiple-stage mass spectrometry analysis
title Advances in structure elucidation of low molecular weight carbohydrates by liquid chromatography-multiple-stage mass spectrometry analysis
spellingShingle Advances in structure elucidation of low molecular weight carbohydrates by liquid chromatography-multiple-stage mass spectrometry analysis
Martín-Ortiz, Andrea
Disaccharides
Trisaccharides
Liquid chromatography-multiple-stage mass spectrometry
Glycosidic linkage
Monomeric units
Diagnostic fragment ions
title_short Advances in structure elucidation of low molecular weight carbohydrates by liquid chromatography-multiple-stage mass spectrometry analysis
title_full Advances in structure elucidation of low molecular weight carbohydrates by liquid chromatography-multiple-stage mass spectrometry analysis
title_fullStr Advances in structure elucidation of low molecular weight carbohydrates by liquid chromatography-multiple-stage mass spectrometry analysis
title_full_unstemmed Advances in structure elucidation of low molecular weight carbohydrates by liquid chromatography-multiple-stage mass spectrometry analysis
title_sort Advances in structure elucidation of low molecular weight carbohydrates by liquid chromatography-multiple-stage mass spectrometry analysis
dc.creator.none.fl_str_mv Martín-Ortiz, Andrea
Carrero-Carralero, Cipriano
Hernández-Hernández, Oswaldo
Lebrón-Aguilar, Rosa
Moreno, F. Javier
Sanz, M. Luz
Ruiz-Matute, Ana I.
author Martín-Ortiz, Andrea
author_facet Martín-Ortiz, Andrea
Carrero-Carralero, Cipriano
Hernández-Hernández, Oswaldo
Lebrón-Aguilar, Rosa
Moreno, F. Javier
Sanz, M. Luz
Ruiz-Matute, Ana I.
author_role author
author2 Carrero-Carralero, Cipriano
Hernández-Hernández, Oswaldo
Lebrón-Aguilar, Rosa
Moreno, F. Javier
Sanz, M. Luz
Ruiz-Matute, Ana I.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
European Commission
Ministerio de Economía, Industria y Competitividad (España)
Comunidad de Madrid
Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Disaccharides
Trisaccharides
Liquid chromatography-multiple-stage mass spectrometry
Glycosidic linkage
Monomeric units
Diagnostic fragment ions
topic Disaccharides
Trisaccharides
Liquid chromatography-multiple-stage mass spectrometry
Glycosidic linkage
Monomeric units
Diagnostic fragment ions
description Oligosaccharides are gaining importance because of their beneficial properties in human health. They normally appear in natural and synthetic products as complex mixtures of different monomeric units, glycosidic linkages and degrees of polymerization, being disaccharides and trisaccharides usually the most abundant ones. Although liquid chromatography-mass spectrometry is a useful technique for oligosaccharides analysis, the similarity of their structures makes difficult their characterization. Moreover, there is still scarce information about the relationship between carbohydrate chemical structure, mass spectra and chromatographic data. Then, in this work, chromatographic parameters for 23 disaccharides with different linkages and monomeric units (glucose, galactose, mannose and fructose) were determined using porous graphitized and hydrophilic interaction liquid chromatography columns. Moreover, diagnostic ions of these disaccharides obtained by tandem mass spectra (MS) were established by stepwise linear discriminant analysis. The relationship between carbohydrate chemical structure and their chromatographic retention data and characteristic ions obtained by multiple-stage mass spectrometry (MS) was successful in establishing some specific criteria that allowed the characterization of trisaccharides with different structural features.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/204495
url http://hdl.handle.net/10261/204495
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016-80475-R
S2018/BAA-4393/AVANSECAL-II-CM
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/AGL2017-84614-C2-1-R
AGL2017-84614-C2-1-R /AEI/10.13039/501100011033
http://dx.doi.org/10.1016/j.chroma.2019.460664
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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