B-cell death and mass in syngeneically transplanted islets exposed to short and long-term hyperglycemia

We studied the effects of hyperglycemia on beta-cell death and mass in syngeneically transplanted islets. Six groups of STZ-induced diabetic C57BL/6 mice were transplanted with 100 syngeneic islets, an insufficient beta-cell mass to restore normoglycemia. Groups 1, 2, and 3 remained hyperglycemic th...

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Autores: Biarnés Costa, Montse, Montolio Rusiñol, Marta, Nacher, Victor, Raurell, Mercè, Soler Ramon, Joan, Montanya Mias, Eduard
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2002
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/135009
Acceso en línea:https://hdl.handle.net/2445/135009
Access Level:acceso abierto
Palabra clave:Diabetis
Cirurgia experimental
Hiperglucèmia
Illots de Langerhans
Diabetes
Experimental surgery
Hyperglycemia
Islands of Langerhans
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spelling B-cell death and mass in syngeneically transplanted islets exposed to short and long-term hyperglycemiaBiarnés Costa, MontseMontolio Rusiñol, MartaNacher, VictorRaurell, MercèSoler Ramon, JoanMontanya Mias, EduardDiabetisCirurgia experimentalHiperglucèmiaIllots de LangerhansDiabetesExperimental surgeryHyperglycemiaIslands of LangerhansWe studied the effects of hyperglycemia on beta-cell death and mass in syngeneically transplanted islets. Six groups of STZ-induced diabetic C57BL/6 mice were transplanted with 100 syngeneic islets, an insufficient beta-cell mass to restore normoglycemia. Groups 1, 2, and 3 remained hyperglycemic throughout the study. Groups 4, 5, and 6 were treated with insulin from day 7 before transplantation to day 10 after transplantation. After insulin discontinuation, group 6 mice achieved definitive normoglycemia. Grafts were harvested at 3 (groups 1 and 4), 10 (groups 2 and 5), and 30 (groups 3 and 6) days after transplantation. On day 3, the initially transplanted beta-cell mass (0.13 +/- 0.01 mg) was dramatically and similarly reduced in the hyperglycemic and insulin-treated groups (group 1: 0.048 +/- 0.002 mg; group 4: 0.046 +/- 0.007 mg; P < 0.001). Extensive islet necrosis (group 1: 30.7%; group 4: 26.8%) and increased beta-cell apoptosis (group 1: 0.30 +/- 0.05%; group 4: 0.42 +/- 0.07%) were found. On day 10, apoptosis remained increased in both hyperglycemic and insulin-treated mice (group 2: 0.44 +/- 0.09%; group 5: 0.48 +/- 0.08%) compared with normal pancreas (0.04 +/- 0.03%; P < 0.001). In contrast, on day 30, beta-cell apoptosis was increased in grafts exposed to sustained hyperglycemia (group 3: 0.37 +/- 0.03%) but not in normoglycemic mice (group 6: 0.12 +/- 0.02%); beta-cell mass was selectively reduced in islets exposed to hyperglycemia (group 3: 0.046 +/- 0.02 mg; group 6: 0.102 +/- 0.009 mg; P < 0.01). In summary, even in optimal conditions, approximately 60% of transplanted islet tissue was lost 3 days after syngeneic transplantation, and both apoptosis and necrosis contributed to beta-cell death. Increased apoptosis and reduced beta-cell mass were also found in islets exposed to chronic hyperglycemia, suggesting that sustained hyperglycemia increased apoptosis in transplanted beta-cells.American Diabetes Association2002info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/135009Articles publicats en revistes (Ciències Clíniques)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.2337/diabetes.51.1.66Diabetes, 2002, vol. 51, num. 1, p. 66-72https://doi.org/10.2337/diabetes.51.1.66cc-by-nc-nd (c) American Diabetes Association, 2002http://creativecommons.org/licenses/by-nc-nd/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1350092026-05-27T06:46:51Z
dc.title.none.fl_str_mv B-cell death and mass in syngeneically transplanted islets exposed to short and long-term hyperglycemia
title B-cell death and mass in syngeneically transplanted islets exposed to short and long-term hyperglycemia
spellingShingle B-cell death and mass in syngeneically transplanted islets exposed to short and long-term hyperglycemia
Biarnés Costa, Montse
Diabetis
Cirurgia experimental
Hiperglucèmia
Illots de Langerhans
Diabetes
Experimental surgery
Hyperglycemia
Islands of Langerhans
title_short B-cell death and mass in syngeneically transplanted islets exposed to short and long-term hyperglycemia
title_full B-cell death and mass in syngeneically transplanted islets exposed to short and long-term hyperglycemia
title_fullStr B-cell death and mass in syngeneically transplanted islets exposed to short and long-term hyperglycemia
title_full_unstemmed B-cell death and mass in syngeneically transplanted islets exposed to short and long-term hyperglycemia
title_sort B-cell death and mass in syngeneically transplanted islets exposed to short and long-term hyperglycemia
dc.creator.none.fl_str_mv Biarnés Costa, Montse
Montolio Rusiñol, Marta
Nacher, Victor
Raurell, Mercè
Soler Ramon, Joan
Montanya Mias, Eduard
author Biarnés Costa, Montse
author_facet Biarnés Costa, Montse
Montolio Rusiñol, Marta
Nacher, Victor
Raurell, Mercè
Soler Ramon, Joan
Montanya Mias, Eduard
author_role author
author2 Montolio Rusiñol, Marta
Nacher, Victor
Raurell, Mercè
Soler Ramon, Joan
Montanya Mias, Eduard
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Diabetis
Cirurgia experimental
Hiperglucèmia
Illots de Langerhans
Diabetes
Experimental surgery
Hyperglycemia
Islands of Langerhans
topic Diabetis
Cirurgia experimental
Hiperglucèmia
Illots de Langerhans
Diabetes
Experimental surgery
Hyperglycemia
Islands of Langerhans
description We studied the effects of hyperglycemia on beta-cell death and mass in syngeneically transplanted islets. Six groups of STZ-induced diabetic C57BL/6 mice were transplanted with 100 syngeneic islets, an insufficient beta-cell mass to restore normoglycemia. Groups 1, 2, and 3 remained hyperglycemic throughout the study. Groups 4, 5, and 6 were treated with insulin from day 7 before transplantation to day 10 after transplantation. After insulin discontinuation, group 6 mice achieved definitive normoglycemia. Grafts were harvested at 3 (groups 1 and 4), 10 (groups 2 and 5), and 30 (groups 3 and 6) days after transplantation. On day 3, the initially transplanted beta-cell mass (0.13 +/- 0.01 mg) was dramatically and similarly reduced in the hyperglycemic and insulin-treated groups (group 1: 0.048 +/- 0.002 mg; group 4: 0.046 +/- 0.007 mg; P < 0.001). Extensive islet necrosis (group 1: 30.7%; group 4: 26.8%) and increased beta-cell apoptosis (group 1: 0.30 +/- 0.05%; group 4: 0.42 +/- 0.07%) were found. On day 10, apoptosis remained increased in both hyperglycemic and insulin-treated mice (group 2: 0.44 +/- 0.09%; group 5: 0.48 +/- 0.08%) compared with normal pancreas (0.04 +/- 0.03%; P < 0.001). In contrast, on day 30, beta-cell apoptosis was increased in grafts exposed to sustained hyperglycemia (group 3: 0.37 +/- 0.03%) but not in normoglycemic mice (group 6: 0.12 +/- 0.02%); beta-cell mass was selectively reduced in islets exposed to hyperglycemia (group 3: 0.046 +/- 0.02 mg; group 6: 0.102 +/- 0.009 mg; P < 0.01). In summary, even in optimal conditions, approximately 60% of transplanted islet tissue was lost 3 days after syngeneic transplantation, and both apoptosis and necrosis contributed to beta-cell death. Increased apoptosis and reduced beta-cell mass were also found in islets exposed to chronic hyperglycemia, suggesting that sustained hyperglycemia increased apoptosis in transplanted beta-cells.
publishDate 2002
dc.date.none.fl_str_mv 2002
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/135009
url https://hdl.handle.net/2445/135009
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.2337/diabetes.51.1.66
Diabetes, 2002, vol. 51, num. 1, p. 66-72
https://doi.org/10.2337/diabetes.51.1.66
dc.rights.none.fl_str_mv cc-by-nc-nd (c) American Diabetes Association, 2002
http://creativecommons.org/licenses/by-nc-nd/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) American Diabetes Association, 2002
http://creativecommons.org/licenses/by-nc-nd/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Diabetes Association
publisher.none.fl_str_mv American Diabetes Association
dc.source.none.fl_str_mv Articles publicats en revistes (Ciències Clíniques)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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