Structural rearrangements detected in newly-formed hexaploid tritordeum after three sequential FISH experiments with repetitive DNA sequences
Mitotic chromosomes of tritordeum (HchHchAABB; 2n = 6x = 42; HT27) and their respective parents, Hordeum chilense L. (HchHch; 2n = 2x = 14; H7) and Triticum turgidum (AABB; 2n = 4x = 28; T81), were analysed after three sequential fluorescence in situ hybridization (FISH) experiments performed with s...
| Autores: | , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2014 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/101244 |
| Acceso en línea: | http://hdl.handle.net/10261/101244 |
| Access Level: | acceso abierto |
| Palabra clave: | Structural rearrangements Simple sequence repeats Fluorescence in situ hybridization Tritordeum Wheat Multicolour FISH Nondenaturing FISH |
| Sumario: | Mitotic chromosomes of tritordeum (HchHchAABB; 2n = 6x = 42; HT27) and their respective parents, Hordeum chilense L. (HchHch; 2n = 2x = 14; H7) and Triticum turgidum (AABB; 2n = 4x = 28; T81), were analysed after three sequential fluorescence in situ hybridization (FISH) experiments performed with six probes: the simple sequence repeats (SSRs) (AG)10 and (AAC)5; genomic DNA from H. chilense; 45S rDNA (pTa71); 5S rDNA (pTa794); and pSc119.2, to achieve a comparative analysis about the distribution of these sequences in the three plant materials. The SSR (AG)10 probe showed eight hybridization signals on tritordeum chromosomes and six in the chromosomes of its male parent (durum wheat). No hybridization signals were detected in female parent H. chilense. |
|---|