Performance comparison of a flow cytometry immunoassay for intracellular cytokine staining and the QuantiFERON® SARS-CoV-2 test for detection and quantification of SARS-CoV-2-Spike-reactive-IFN-?-producing T cells after COVID-19 vaccination.

PURPOSE: We compared the performance of an in-house-developed flow cytometry assay for intracellular cytokine staining (FC-ICS) and a commercially-available cytokine release assay (the QuantiFERON® SARS-CoV-2 Test [QF]) for detection and quantification of SARS-CoV-2-Spike (S)-reactive-IFN-?-producin...

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Autores: Tormo, Nuria, Gimenez, Estela, Martinez-Navarro, Maria, Albert, Eliseo, Navalpotro, David, Torres, Ignacio, Gimeno, Concepcion, Navarro, David
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Recursos:INCLIVA
Repositorio:r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
OAI Identifier:oai:incliva.fundanetsuite.com:p16412
Acesso em linha:https://incliva.portalinvestigacion.com/publicaciones/16412
Access Level:acceso abierto
Palavra-chave:COVID-19 vaccine
Flow cytometry-based immunoassay
QuantiFERON® SARS-CoV-2
SARS-CoV-2 S protein
SARS-CoV-2 T cells
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spelling Performance comparison of a flow cytometry immunoassay for intracellular cytokine staining and the QuantiFERON® SARS-CoV-2 test for detection and quantification of SARS-CoV-2-Spike-reactive-IFN-?-producing T cells after COVID-19 vaccination.Tormo, NuriaGimenez, EstelaMartinez-Navarro, MariaAlbert, EliseoNavalpotro, DavidTorres, IgnacioGimeno, ConcepcionNavarro, DavidCOVID-19 vaccineFlow cytometry-based immunoassayQuantiFERON® SARS-CoV-2SARS-CoV-2 S proteinSARS-CoV-2 T cellsPURPOSE: We compared the performance of an in-house-developed flow cytometry assay for intracellular cytokine staining (FC-ICS) and a commercially-available cytokine release assay (the QuantiFERON® SARS-CoV-2 Test [QF]) for detection and quantification of SARS-CoV-2-Spike (S)-reactive-IFN-?-producing T cells after COVID-19 vaccination. PATIENTS AND METHODS: The sample included 141 individuals (all male; median age, 42 years; 20-72) who had been fully vaccinated with the Comirnaty® COVID-19 vaccine (at a median of 114 days; 34-145). Prior to vaccination, 91 were categorized as being SARS-CoV-2-naïve and 50 as SARS-CoV-2-experienced. A whole blood-based FC-ICS using 15-mer overlapping peptides encompassing the entire SARS-CoV-2 S protein was used for enumeration of virus-specific IFN-?-producing CD4(+) and CD8(+) T cells. The QF test (Ag1 for CD4(+) T cells and Ag2 for CD4(+) and CD8(+) T cells in combination) was carried out following the manufacturer's instructions. RESULTS: The FC-ICS and the QF assays returned significantly discordant qualitative results in both the entire cohort (P<0.001 with QF Ag1 and QF Ag2) and in SARS-CoV-2-naïve participants alone (P=0.005 and P=0.01, respectively). Discrepant results mostly involved FC-ICS positive/QF negative specimens. Overall, no correlation was found either between SARS-CoV-2 IFN-?- CD4(+) T-cell frequencies and IFN-? levels measured in the QF Ag1 tube (P=0.78) or between the sum of SARS-CoV-2 IFN-? CD4(+) and CD8(+) T-cell frequencies and IFN-? levels quantified in the QF Ag2 tube. CONCLUSION: The data suggest a greater sensitivity for the FC-ICS assay than the QF test, and urge caution when comparing SARS-CoV-2 T-cell immune responses assessed using different analytical platforms.SPRINGER2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://incliva.portalinvestigacion.com/publicaciones/16412EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASESISSN: 09349723ISSNe: 14354373reponame:r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVAinstname:INCLIVAInglésinfo:eu-repo/semantics/openAccessoai:incliva.fundanetsuite.com:p164122026-06-07T16:35:31Z
dc.title.none.fl_str_mv Performance comparison of a flow cytometry immunoassay for intracellular cytokine staining and the QuantiFERON® SARS-CoV-2 test for detection and quantification of SARS-CoV-2-Spike-reactive-IFN-?-producing T cells after COVID-19 vaccination.
title Performance comparison of a flow cytometry immunoassay for intracellular cytokine staining and the QuantiFERON® SARS-CoV-2 test for detection and quantification of SARS-CoV-2-Spike-reactive-IFN-?-producing T cells after COVID-19 vaccination.
spellingShingle Performance comparison of a flow cytometry immunoassay for intracellular cytokine staining and the QuantiFERON® SARS-CoV-2 test for detection and quantification of SARS-CoV-2-Spike-reactive-IFN-?-producing T cells after COVID-19 vaccination.
Tormo, Nuria
COVID-19 vaccine
Flow cytometry-based immunoassay
QuantiFERON® SARS-CoV-2
SARS-CoV-2 S protein
SARS-CoV-2 T cells
title_short Performance comparison of a flow cytometry immunoassay for intracellular cytokine staining and the QuantiFERON® SARS-CoV-2 test for detection and quantification of SARS-CoV-2-Spike-reactive-IFN-?-producing T cells after COVID-19 vaccination.
title_full Performance comparison of a flow cytometry immunoassay for intracellular cytokine staining and the QuantiFERON® SARS-CoV-2 test for detection and quantification of SARS-CoV-2-Spike-reactive-IFN-?-producing T cells after COVID-19 vaccination.
title_fullStr Performance comparison of a flow cytometry immunoassay for intracellular cytokine staining and the QuantiFERON® SARS-CoV-2 test for detection and quantification of SARS-CoV-2-Spike-reactive-IFN-?-producing T cells after COVID-19 vaccination.
title_full_unstemmed Performance comparison of a flow cytometry immunoassay for intracellular cytokine staining and the QuantiFERON® SARS-CoV-2 test for detection and quantification of SARS-CoV-2-Spike-reactive-IFN-?-producing T cells after COVID-19 vaccination.
title_sort Performance comparison of a flow cytometry immunoassay for intracellular cytokine staining and the QuantiFERON® SARS-CoV-2 test for detection and quantification of SARS-CoV-2-Spike-reactive-IFN-?-producing T cells after COVID-19 vaccination.
dc.creator.none.fl_str_mv Tormo, Nuria
Gimenez, Estela
Martinez-Navarro, Maria
Albert, Eliseo
Navalpotro, David
Torres, Ignacio
Gimeno, Concepcion
Navarro, David
author Tormo, Nuria
author_facet Tormo, Nuria
Gimenez, Estela
Martinez-Navarro, Maria
Albert, Eliseo
Navalpotro, David
Torres, Ignacio
Gimeno, Concepcion
Navarro, David
author_role author
author2 Gimenez, Estela
Martinez-Navarro, Maria
Albert, Eliseo
Navalpotro, David
Torres, Ignacio
Gimeno, Concepcion
Navarro, David
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv COVID-19 vaccine
Flow cytometry-based immunoassay
QuantiFERON® SARS-CoV-2
SARS-CoV-2 S protein
SARS-CoV-2 T cells
topic COVID-19 vaccine
Flow cytometry-based immunoassay
QuantiFERON® SARS-CoV-2
SARS-CoV-2 S protein
SARS-CoV-2 T cells
description PURPOSE: We compared the performance of an in-house-developed flow cytometry assay for intracellular cytokine staining (FC-ICS) and a commercially-available cytokine release assay (the QuantiFERON® SARS-CoV-2 Test [QF]) for detection and quantification of SARS-CoV-2-Spike (S)-reactive-IFN-?-producing T cells after COVID-19 vaccination. PATIENTS AND METHODS: The sample included 141 individuals (all male; median age, 42 years; 20-72) who had been fully vaccinated with the Comirnaty® COVID-19 vaccine (at a median of 114 days; 34-145). Prior to vaccination, 91 were categorized as being SARS-CoV-2-naïve and 50 as SARS-CoV-2-experienced. A whole blood-based FC-ICS using 15-mer overlapping peptides encompassing the entire SARS-CoV-2 S protein was used for enumeration of virus-specific IFN-?-producing CD4(+) and CD8(+) T cells. The QF test (Ag1 for CD4(+) T cells and Ag2 for CD4(+) and CD8(+) T cells in combination) was carried out following the manufacturer's instructions. RESULTS: The FC-ICS and the QF assays returned significantly discordant qualitative results in both the entire cohort (P<0.001 with QF Ag1 and QF Ag2) and in SARS-CoV-2-naïve participants alone (P=0.005 and P=0.01, respectively). Discrepant results mostly involved FC-ICS positive/QF negative specimens. Overall, no correlation was found either between SARS-CoV-2 IFN-?- CD4(+) T-cell frequencies and IFN-? levels measured in the QF Ag1 tube (P=0.78) or between the sum of SARS-CoV-2 IFN-? CD4(+) and CD8(+) T-cell frequencies and IFN-? levels quantified in the QF Ag2 tube. CONCLUSION: The data suggest a greater sensitivity for the FC-ICS assay than the QF test, and urge caution when comparing SARS-CoV-2 T-cell immune responses assessed using different analytical platforms.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://incliva.portalinvestigacion.com/publicaciones/16412
url https://incliva.portalinvestigacion.com/publicaciones/16412
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv SPRINGER
publisher.none.fl_str_mv SPRINGER
dc.source.none.fl_str_mv EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
ISSN: 09349723
ISSNe: 14354373
reponame:r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
instname:INCLIVA
instname_str INCLIVA
reponame_str r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
collection r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
repository.name.fl_str_mv
repository.mail.fl_str_mv
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