Multiparametric profiling of engineered nanomaterials

Despite considerable efforts, the properties that drive the cytotoxicity of engineered nanomaterials (ENMs) remain poorly understood. Here, the authors inverstigate a panel of 31 ENMs with different core chemistries and a variety of surface modifications using conventional in vitro assays coupled wi...

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Autores: Gallud, Aucrey, Delaval, Mathilde, Kinaret, Pia, Marwah, Veer Singh, Fortino, Vittorio, Ytterberg, Jimmy, Zubarev, Roman, Skoog, Tiina, Kere, Juha|||0000-0003-1974-0271, Correia, Manuel, Loeschner, Katrin, Al-Ahmady, Zahraa, Kostarelos, Kostas|||0000-0002-2224-6672, Ruiz, Jaime, Astruc, Didier, Monopoli, Marco, Handy, Richard D, Moya, Sergio|||0000-0002-7174-1960, Savolainen, Kai, Alenius, Harri|||0000-0003-0106-8923, Greco, Dario, Fadeel, Bengt|||0000-0001-5559-8482
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:250129
Acceso en línea:https://ddd.uab.cat/record/250129
https://dx.doi.org/urn:doi:10.1002/advs.202002221
Access Level:acceso abierto
Palabra clave:Immunotoxicity
Nanomaterials
Nanotoxicology
Proteomics
Transcriptomics
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spelling Multiparametric profiling of engineered nanomaterialsunmasking the surface coating effectGallud, AucreyDelaval, MathildeKinaret, PiaMarwah, Veer SinghFortino, VittorioYtterberg, JimmyZubarev, RomanSkoog, TiinaKere, Juha|||0000-0003-1974-0271Correia, ManuelLoeschner, KatrinAl-Ahmady, ZahraaKostarelos, Kostas|||0000-0002-2224-6672Ruiz, JaimeAstruc, DidierMonopoli, MarcoHandy, Richard DMoya, Sergio|||0000-0002-7174-1960Savolainen, KaiAlenius, Harri|||0000-0003-0106-8923Greco, DarioFadeel, Bengt|||0000-0001-5559-8482ImmunotoxicityNanomaterialsNanotoxicologyProteomicsTranscriptomicsDespite considerable efforts, the properties that drive the cytotoxicity of engineered nanomaterials (ENMs) remain poorly understood. Here, the authors inverstigate a panel of 31 ENMs with different core chemistries and a variety of surface modifications using conventional in vitro assays coupled with omics-based approaches. Cytotoxicity screening and multiplex-based cytokine profiling reveals a good concordance between primary human monocyte-derived macrophages and the human monocyte-like cell line THP-1. Proteomics analysis following a low-dose exposure of cells suggests a nonspecific stress response to ENMs, while microarray-based profiling reveals significant changes in gene expression as a function of both surface modification and core chemistry. Pathway analysis highlights that the ENMs with cationic surfaces that are shown to elicit cytotoxicity downregulated DNA replication and cell cycle responses, while inflammatory responses are upregulated. These findings are validated using cell-based assays. Notably, certain small, PEGylated ENMs are found to be noncytotoxic yet they induce transcriptional responses reminiscent of viruses. In sum, using a multiparametric approach, it is shown that surface chemistry is a key determinant of cellular responses to ENMs. The data also reveal that cytotoxicity, determined by conventional in vitro assays, does not necessarily correlate with transcriptional effects of ENMs. 22020-01-0120202020-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/250129https://dx.doi.org/urn:doi:10.1002/advs.202002221reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengEuropean Commission https://doi.org/10.13039/501100000780 309329open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2501292026-06-06T12:50:31Z
dc.title.none.fl_str_mv Multiparametric profiling of engineered nanomaterials
unmasking the surface coating effect
title Multiparametric profiling of engineered nanomaterials
spellingShingle Multiparametric profiling of engineered nanomaterials
Gallud, Aucrey
Immunotoxicity
Nanomaterials
Nanotoxicology
Proteomics
Transcriptomics
title_short Multiparametric profiling of engineered nanomaterials
title_full Multiparametric profiling of engineered nanomaterials
title_fullStr Multiparametric profiling of engineered nanomaterials
title_full_unstemmed Multiparametric profiling of engineered nanomaterials
title_sort Multiparametric profiling of engineered nanomaterials
dc.creator.none.fl_str_mv Gallud, Aucrey
Delaval, Mathilde
Kinaret, Pia
Marwah, Veer Singh
Fortino, Vittorio
Ytterberg, Jimmy
Zubarev, Roman
Skoog, Tiina
Kere, Juha|||0000-0003-1974-0271
Correia, Manuel
Loeschner, Katrin
Al-Ahmady, Zahraa
Kostarelos, Kostas|||0000-0002-2224-6672
Ruiz, Jaime
Astruc, Didier
Monopoli, Marco
Handy, Richard D
Moya, Sergio|||0000-0002-7174-1960
Savolainen, Kai
Alenius, Harri|||0000-0003-0106-8923
Greco, Dario
Fadeel, Bengt|||0000-0001-5559-8482
author Gallud, Aucrey
author_facet Gallud, Aucrey
Delaval, Mathilde
Kinaret, Pia
Marwah, Veer Singh
Fortino, Vittorio
Ytterberg, Jimmy
Zubarev, Roman
Skoog, Tiina
Kere, Juha|||0000-0003-1974-0271
Correia, Manuel
Loeschner, Katrin
Al-Ahmady, Zahraa
Kostarelos, Kostas|||0000-0002-2224-6672
Ruiz, Jaime
Astruc, Didier
Monopoli, Marco
Handy, Richard D
Moya, Sergio|||0000-0002-7174-1960
Savolainen, Kai
Alenius, Harri|||0000-0003-0106-8923
Greco, Dario
Fadeel, Bengt|||0000-0001-5559-8482
author_role author
author2 Delaval, Mathilde
Kinaret, Pia
Marwah, Veer Singh
Fortino, Vittorio
Ytterberg, Jimmy
Zubarev, Roman
Skoog, Tiina
Kere, Juha|||0000-0003-1974-0271
Correia, Manuel
Loeschner, Katrin
Al-Ahmady, Zahraa
Kostarelos, Kostas|||0000-0002-2224-6672
Ruiz, Jaime
Astruc, Didier
Monopoli, Marco
Handy, Richard D
Moya, Sergio|||0000-0002-7174-1960
Savolainen, Kai
Alenius, Harri|||0000-0003-0106-8923
Greco, Dario
Fadeel, Bengt|||0000-0001-5559-8482
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Immunotoxicity
Nanomaterials
Nanotoxicology
Proteomics
Transcriptomics
topic Immunotoxicity
Nanomaterials
Nanotoxicology
Proteomics
Transcriptomics
description Despite considerable efforts, the properties that drive the cytotoxicity of engineered nanomaterials (ENMs) remain poorly understood. Here, the authors inverstigate a panel of 31 ENMs with different core chemistries and a variety of surface modifications using conventional in vitro assays coupled with omics-based approaches. Cytotoxicity screening and multiplex-based cytokine profiling reveals a good concordance between primary human monocyte-derived macrophages and the human monocyte-like cell line THP-1. Proteomics analysis following a low-dose exposure of cells suggests a nonspecific stress response to ENMs, while microarray-based profiling reveals significant changes in gene expression as a function of both surface modification and core chemistry. Pathway analysis highlights that the ENMs with cationic surfaces that are shown to elicit cytotoxicity downregulated DNA replication and cell cycle responses, while inflammatory responses are upregulated. These findings are validated using cell-based assays. Notably, certain small, PEGylated ENMs are found to be noncytotoxic yet they induce transcriptional responses reminiscent of viruses. In sum, using a multiparametric approach, it is shown that surface chemistry is a key determinant of cellular responses to ENMs. The data also reveal that cytotoxicity, determined by conventional in vitro assays, does not necessarily correlate with transcriptional effects of ENMs.
publishDate 2020
dc.date.none.fl_str_mv 2
2020-01-01
2020
2020-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/250129
https://dx.doi.org/urn:doi:10.1002/advs.202002221
url https://ddd.uab.cat/record/250129
https://dx.doi.org/urn:doi:10.1002/advs.202002221
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv European Commission https://doi.org/10.13039/501100000780 309329
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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