Metabolomics and transcriptomics to decipher molecular mechanisms underlying ectomycorrhizal root colonization of an oak tree
Mycorrhizas are known to have a positive impact on plant growth and ability to resist major biotic and abiotic stresses. However, the metabolic alterations underlying mycorrhizal symbiosis are still understudied. By using metabolomics and transcriptomics approaches, cork oak roots colonized by the e...
| Autores: | , , , , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:255474 |
| Acceso en línea: | https://ddd.uab.cat/record/255474 https://dx.doi.org/urn:doi:10.1038/s41598-021-87886-5 |
| Access Level: | acceso abierto |
| Palabra clave: | Molecular biology Plant sciences |
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Metabolomics and transcriptomics to decipher molecular mechanisms underlying ectomycorrhizal root colonization of an oak treeSebastiana, Mónica|||0000-0001-8735-7632Gargallo-Garriga, Albert|||0000-0002-7536-2888Sardans i Galobart, Jordi|||0000-0003-2478-0219Pérez-Trujillo, Míriam|||0000-0002-6919-7417Monteiro, Filipa|||0000-0002-1505-2140Figueiredo, Andreia|||0000-0001-8156-7700Maia, Marisa|||0000-0003-3202-0797Nascimento, Rui|||0000-0002-0478-336XSousa Silva, Marta|||0000-0002-3080-9682Ferreira, António E N.|||0000-0002-9625-8115Cordeiro, Carlos|||0000-0001-6327-8606Marques, Ana Patrícia|||0000-0002-7393-8556Sousa, Lisete|||0000-0002-2114-720XMalhó, Rui|||0000-0001-5287-869XPeñuelas, Josep|||0000-0002-7215-0150Molecular biologyPlant sciencesMycorrhizas are known to have a positive impact on plant growth and ability to resist major biotic and abiotic stresses. However, the metabolic alterations underlying mycorrhizal symbiosis are still understudied. By using metabolomics and transcriptomics approaches, cork oak roots colonized by the ectomycorrhizal fungus Pisolithus tinctorius were compared with non-colonized roots. Results show that compounds putatively corresponding to carbohydrates, organic acids, tannins, long-chain fatty acids and monoacylglycerols, were depleted in ectomycorrhizal cork oak colonized roots. Conversely, non-proteogenic amino acids, such as gamma-aminobutyric acid (GABA), and several putative defense-related compounds, including oxylipin-family compounds, terpenoids and B6 vitamers were induced in mycorrhizal roots. Transcriptomic analysis suggests the involvement of GABA in ectomycorrhizal symbiosis through increased synthesis and inhibition of degradation in mycorrhizal roots. Results from this global metabolomics analysis suggest decreases in root metabolites which are common components of exudates, and in compounds related to root external protective layers which could facilitate plant-fungal contact and enhance symbiosis. Root metabolic pathways involved in defense against stress were induced in ectomycorrhizal roots that could be involved in a plant mechanism to avoid uncontrolled growth of the fungal symbiont in the root apoplast. Several of the identified symbiosis-specific metabolites, such as GABA, may help to understand how ectomycorrhizal fungi such as P. tinctorius benefit their host plants. 22021-01-0120212021-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/255474https://dx.doi.org/urn:doi:10.1038/s41598-021-87886-5reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengEuropean Commission https://doi.org/10.13039/501100000780 731077open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2554742026-06-06T12:50:31Z |
| dc.title.none.fl_str_mv |
Metabolomics and transcriptomics to decipher molecular mechanisms underlying ectomycorrhizal root colonization of an oak tree |
| title |
Metabolomics and transcriptomics to decipher molecular mechanisms underlying ectomycorrhizal root colonization of an oak tree |
| spellingShingle |
Metabolomics and transcriptomics to decipher molecular mechanisms underlying ectomycorrhizal root colonization of an oak tree Sebastiana, Mónica|||0000-0001-8735-7632 Molecular biology Plant sciences |
| title_short |
Metabolomics and transcriptomics to decipher molecular mechanisms underlying ectomycorrhizal root colonization of an oak tree |
| title_full |
Metabolomics and transcriptomics to decipher molecular mechanisms underlying ectomycorrhizal root colonization of an oak tree |
| title_fullStr |
Metabolomics and transcriptomics to decipher molecular mechanisms underlying ectomycorrhizal root colonization of an oak tree |
| title_full_unstemmed |
Metabolomics and transcriptomics to decipher molecular mechanisms underlying ectomycorrhizal root colonization of an oak tree |
| title_sort |
Metabolomics and transcriptomics to decipher molecular mechanisms underlying ectomycorrhizal root colonization of an oak tree |
| dc.creator.none.fl_str_mv |
Sebastiana, Mónica|||0000-0001-8735-7632 Gargallo-Garriga, Albert|||0000-0002-7536-2888 Sardans i Galobart, Jordi|||0000-0003-2478-0219 Pérez-Trujillo, Míriam|||0000-0002-6919-7417 Monteiro, Filipa|||0000-0002-1505-2140 Figueiredo, Andreia|||0000-0001-8156-7700 Maia, Marisa|||0000-0003-3202-0797 Nascimento, Rui|||0000-0002-0478-336X Sousa Silva, Marta|||0000-0002-3080-9682 Ferreira, António E N.|||0000-0002-9625-8115 Cordeiro, Carlos|||0000-0001-6327-8606 Marques, Ana Patrícia|||0000-0002-7393-8556 Sousa, Lisete|||0000-0002-2114-720X Malhó, Rui|||0000-0001-5287-869X Peñuelas, Josep|||0000-0002-7215-0150 |
| author |
Sebastiana, Mónica|||0000-0001-8735-7632 |
| author_facet |
Sebastiana, Mónica|||0000-0001-8735-7632 Gargallo-Garriga, Albert|||0000-0002-7536-2888 Sardans i Galobart, Jordi|||0000-0003-2478-0219 Pérez-Trujillo, Míriam|||0000-0002-6919-7417 Monteiro, Filipa|||0000-0002-1505-2140 Figueiredo, Andreia|||0000-0001-8156-7700 Maia, Marisa|||0000-0003-3202-0797 Nascimento, Rui|||0000-0002-0478-336X Sousa Silva, Marta|||0000-0002-3080-9682 Ferreira, António E N.|||0000-0002-9625-8115 Cordeiro, Carlos|||0000-0001-6327-8606 Marques, Ana Patrícia|||0000-0002-7393-8556 Sousa, Lisete|||0000-0002-2114-720X Malhó, Rui|||0000-0001-5287-869X Peñuelas, Josep|||0000-0002-7215-0150 |
| author_role |
author |
| author2 |
Gargallo-Garriga, Albert|||0000-0002-7536-2888 Sardans i Galobart, Jordi|||0000-0003-2478-0219 Pérez-Trujillo, Míriam|||0000-0002-6919-7417 Monteiro, Filipa|||0000-0002-1505-2140 Figueiredo, Andreia|||0000-0001-8156-7700 Maia, Marisa|||0000-0003-3202-0797 Nascimento, Rui|||0000-0002-0478-336X Sousa Silva, Marta|||0000-0002-3080-9682 Ferreira, António E N.|||0000-0002-9625-8115 Cordeiro, Carlos|||0000-0001-6327-8606 Marques, Ana Patrícia|||0000-0002-7393-8556 Sousa, Lisete|||0000-0002-2114-720X Malhó, Rui|||0000-0001-5287-869X Peñuelas, Josep|||0000-0002-7215-0150 |
| author2_role |
author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Molecular biology Plant sciences |
| topic |
Molecular biology Plant sciences |
| description |
Mycorrhizas are known to have a positive impact on plant growth and ability to resist major biotic and abiotic stresses. However, the metabolic alterations underlying mycorrhizal symbiosis are still understudied. By using metabolomics and transcriptomics approaches, cork oak roots colonized by the ectomycorrhizal fungus Pisolithus tinctorius were compared with non-colonized roots. Results show that compounds putatively corresponding to carbohydrates, organic acids, tannins, long-chain fatty acids and monoacylglycerols, were depleted in ectomycorrhizal cork oak colonized roots. Conversely, non-proteogenic amino acids, such as gamma-aminobutyric acid (GABA), and several putative defense-related compounds, including oxylipin-family compounds, terpenoids and B6 vitamers were induced in mycorrhizal roots. Transcriptomic analysis suggests the involvement of GABA in ectomycorrhizal symbiosis through increased synthesis and inhibition of degradation in mycorrhizal roots. Results from this global metabolomics analysis suggest decreases in root metabolites which are common components of exudates, and in compounds related to root external protective layers which could facilitate plant-fungal contact and enhance symbiosis. Root metabolic pathways involved in defense against stress were induced in ectomycorrhizal roots that could be involved in a plant mechanism to avoid uncontrolled growth of the fungal symbiont in the root apoplast. Several of the identified symbiosis-specific metabolites, such as GABA, may help to understand how ectomycorrhizal fungi such as P. tinctorius benefit their host plants. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2 2021-01-01 2021 2021-01-01 |
| dc.type.none.fl_str_mv |
Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
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article |
| dc.identifier.none.fl_str_mv |
https://ddd.uab.cat/record/255474 https://dx.doi.org/urn:doi:10.1038/s41598-021-87886-5 |
| url |
https://ddd.uab.cat/record/255474 https://dx.doi.org/urn:doi:10.1038/s41598-021-87886-5 |
| dc.language.none.fl_str_mv |
Inglés eng |
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Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
European Commission https://doi.org/10.13039/501100000780 731077 |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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openAccess |
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application/pdf |
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reponame:Dipòsit Digital de Documents de la UAB instname:Universitat Autònoma de Barcelona |
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