Assessment of bone-regeneration using adipose-derived stem cells in critical-size alveolar ridge defects: an experimental study in a dog model
Purpose: To assess bone regeneration potential of a fibronectin- and adipose-derived stem cell-covered ceramic biomaterial in three-wall critical size alveolar ridge defects. Materials and methods: In 18 dogs, four dehiscence-type and critical size defects were created surgically in the edentulous a...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2016 |
| País: | España |
| Institución: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:2445/183026 |
| Acceso en línea: | https://hdl.handle.net/2445/183026 |
| Access Level: | acceso abierto |
| Palabra clave: | Cèl·lules mare Glicoproteïnes Regeneració (Biologia) Ossos Stem cells Glycoproteins Regeneration (Biology) Bones |
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Assessment of bone-regeneration using adipose-derived stem cells in critical-size alveolar ridge defects: an experimental study in a dog modelAlvira González, JoaquínSánchez Garcés, Ma. ÁngelesBarbany, Joan RamonReina del Pozo, ManuelMüller Sánchez, Claudia AlejandraGay Escoda, CosmeCèl·lules mareGlicoproteïnesRegeneració (Biologia)OssosStem cellsGlycoproteinsRegeneration (Biology)BonesPurpose: To assess bone regeneration potential of a fibronectin- and adipose-derived stem cell-covered ceramic biomaterial in three-wall critical size alveolar ridge defects. Materials and methods: In 18 dogs, four dehiscence-type and critical size defects were created surgically in the edentulous alveolar ridge. Defects were randomly regenerated using biomaterials coated with particulate ß-tricalcium phosphate (ß-TCP), ß-TCP with fibronectin (Fn) (ß-TCP-Fn), and ß-TCP with a combination of Fn and autologous adipose-derived stem cells (ADSCs) (ß-TCP-Fn-ADSCs), leaving one defect as control. The animals were divided into three groups according to the time of euthanasia (1, 2, or 3 months of healing). Results: At the time of sacrifice, statistically significant differences between the four types of defects in the total area of bone regeneration, percentage of neoformed bone matrix, medullary space, or contact between particulate biomaterial and neoformed bone matrix were not found. All defects showed a significant increase in neoformed bone matrix as sacrifice was delayed, but a uniform pattern was not followed. Only defects treated with ß-TCP-Fn-ADSCs showed a significant increase in the bone regeneration area when animals sacrificed at 3 months were compared to those sacrificed at 1 month (P = .006). Conclusion: The use of ADSCs in bone regeneration processes of critical size defects of the alveolar ridge did not entail an advantage regarding greater bone regeneration as compared with other biomaterials. However, the use of ß-TCP coated with a combination of Fn and ADSCs appeared to favor stabilization of the regenerated area, allowing a more efficient maintenance of the space at 3 months of healing.Quintessence Publishing Group2022202220162022info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion8 p.application/pdfhttps://hdl.handle.net/2445/183026Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/10.11607/jomi.4190International Journal of Oral & Maxillofacial Implants, 2016, vol. 31, num. 1, p. 196-203https://doi.org/10.11607/jomi.4190(c) Quintessence Publishing Group, 2016info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1830262026-05-29T05:05:01Z |
| dc.title.none.fl_str_mv |
Assessment of bone-regeneration using adipose-derived stem cells in critical-size alveolar ridge defects: an experimental study in a dog model |
| title |
Assessment of bone-regeneration using adipose-derived stem cells in critical-size alveolar ridge defects: an experimental study in a dog model |
| spellingShingle |
Assessment of bone-regeneration using adipose-derived stem cells in critical-size alveolar ridge defects: an experimental study in a dog model Alvira González, Joaquín Cèl·lules mare Glicoproteïnes Regeneració (Biologia) Ossos Stem cells Glycoproteins Regeneration (Biology) Bones |
| title_short |
Assessment of bone-regeneration using adipose-derived stem cells in critical-size alveolar ridge defects: an experimental study in a dog model |
| title_full |
Assessment of bone-regeneration using adipose-derived stem cells in critical-size alveolar ridge defects: an experimental study in a dog model |
| title_fullStr |
Assessment of bone-regeneration using adipose-derived stem cells in critical-size alveolar ridge defects: an experimental study in a dog model |
| title_full_unstemmed |
Assessment of bone-regeneration using adipose-derived stem cells in critical-size alveolar ridge defects: an experimental study in a dog model |
| title_sort |
Assessment of bone-regeneration using adipose-derived stem cells in critical-size alveolar ridge defects: an experimental study in a dog model |
| dc.creator.none.fl_str_mv |
Alvira González, Joaquín Sánchez Garcés, Ma. Ángeles Barbany, Joan Ramon Reina del Pozo, Manuel Müller Sánchez, Claudia Alejandra Gay Escoda, Cosme |
| author |
Alvira González, Joaquín |
| author_facet |
Alvira González, Joaquín Sánchez Garcés, Ma. Ángeles Barbany, Joan Ramon Reina del Pozo, Manuel Müller Sánchez, Claudia Alejandra Gay Escoda, Cosme |
| author_role |
author |
| author2 |
Sánchez Garcés, Ma. Ángeles Barbany, Joan Ramon Reina del Pozo, Manuel Müller Sánchez, Claudia Alejandra Gay Escoda, Cosme |
| author2_role |
author author author author author |
| dc.subject.none.fl_str_mv |
Cèl·lules mare Glicoproteïnes Regeneració (Biologia) Ossos Stem cells Glycoproteins Regeneration (Biology) Bones |
| topic |
Cèl·lules mare Glicoproteïnes Regeneració (Biologia) Ossos Stem cells Glycoproteins Regeneration (Biology) Bones |
| description |
Purpose: To assess bone regeneration potential of a fibronectin- and adipose-derived stem cell-covered ceramic biomaterial in three-wall critical size alveolar ridge defects. Materials and methods: In 18 dogs, four dehiscence-type and critical size defects were created surgically in the edentulous alveolar ridge. Defects were randomly regenerated using biomaterials coated with particulate ß-tricalcium phosphate (ß-TCP), ß-TCP with fibronectin (Fn) (ß-TCP-Fn), and ß-TCP with a combination of Fn and autologous adipose-derived stem cells (ADSCs) (ß-TCP-Fn-ADSCs), leaving one defect as control. The animals were divided into three groups according to the time of euthanasia (1, 2, or 3 months of healing). Results: At the time of sacrifice, statistically significant differences between the four types of defects in the total area of bone regeneration, percentage of neoformed bone matrix, medullary space, or contact between particulate biomaterial and neoformed bone matrix were not found. All defects showed a significant increase in neoformed bone matrix as sacrifice was delayed, but a uniform pattern was not followed. Only defects treated with ß-TCP-Fn-ADSCs showed a significant increase in the bone regeneration area when animals sacrificed at 3 months were compared to those sacrificed at 1 month (P = .006). Conclusion: The use of ADSCs in bone regeneration processes of critical size defects of the alveolar ridge did not entail an advantage regarding greater bone regeneration as compared with other biomaterials. However, the use of ß-TCP coated with a combination of Fn and ADSCs appeared to favor stabilization of the regenerated area, allowing a more efficient maintenance of the space at 3 months of healing. |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016 2022 2022 2022 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion |
| format |
article |
| status_str |
acceptedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/2445/183026 |
| url |
https://hdl.handle.net/2445/183026 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Versió postprint del document publicat a: https://doi.org/10.11607/jomi.4190 International Journal of Oral & Maxillofacial Implants, 2016, vol. 31, num. 1, p. 196-203 https://doi.org/10.11607/jomi.4190 |
| dc.rights.none.fl_str_mv |
(c) Quintessence Publishing Group, 2016 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
(c) Quintessence Publishing Group, 2016 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
8 p. application/pdf |
| dc.publisher.none.fl_str_mv |
Quintessence Publishing Group |
| publisher.none.fl_str_mv |
Quintessence Publishing Group |
| dc.source.none.fl_str_mv |
Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia) reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| instname_str |
Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| reponame_str |
Recercat. Dipósit de la Recerca de Catalunya |
| collection |
Recercat. Dipósit de la Recerca de Catalunya |
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1869420487255261184 |
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15,812429 |