Serum proteome of dogs at subclinical and clinical onset of canine leishmaniosis

The objective of this study was to identify changes in serum proteome in dogs that may occur after an experimental infection at subclinical and clinical stages of canine leishmaniosis (CanL). For this purpose, canine pre- and post-infection with Leishmania infantum serum proteomes in the same dogs w...

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Autores: Franco Martínez, Lorena, Villar Rayo, Margarita María, Tvarijonaviciute, Asta, Escribano, Damíán, Bernal, Luis Jesús, Cerón Madrigal, José Joaquín, Thomas, María del Carmen, Mateos Hernández, Lourdes, Tecles Vicente, Fernando, Fuente García, José de Jesús de la, López López, Manuel Carlos, Martínez Subiela, Silvia
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universidad de Castilla-La Mancha
Repositorio:RUIdeRA. Repositorio Institucional de la UCLM
OAI Identifier:oai:ruidera.uclm.es:10578/23839
Acceso en línea:https://hdl.handle.net/10578/23839
Access Level:acceso abierto
Palabra clave:Biomarkers
Dog diseases
Leishmania
Proteomics
SWATH-MS
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spelling Serum proteome of dogs at subclinical and clinical onset of canine leishmaniosisFranco Martínez, LorenaVillar Rayo, Margarita MaríaTvarijonaviciute, AstaEscribano, DamíánBernal, Luis JesúsCerón Madrigal, José JoaquínThomas, María del CarmenMateos Hernández, LourdesTecles Vicente, FernandoFuente García, José de Jesús de laLópez López, Manuel CarlosMartínez Subiela, SilviaBiomarkersDog diseasesLeishmaniaProteomicsSWATH-MSThe objective of this study was to identify changes in serum proteome in dogs that may occur after an experimental infection at subclinical and clinical stages of canine leishmaniosis (CanL). For this purpose, canine pre- and post-infection with Leishmania infantum serum proteomes in the same dogs were analysed by a high-throughput label-based quantitative LC-MS/MS proteomic approach. A total of 169 proteins were identified, and 74 of them including complement C8 alpha chain, adiponectin, transferrin, sphingomyelin phosphodiesterase acid-like 3A and immunoglobulins showed different modulation between the different stages of CanL. These proteins could be considered as potential serum biomarkers of early diagnostic or disease progression in CanL. Additionally, biological pathways modulated during CanL such as blood coagulation or gonadotropin-releasing hormone receptor were revealed, which could help to understand the pathological mechanisms of the disease.Blackwell Verlag202020202019info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://hdl.handle.net/10578/23839reponame:RUIdeRA. Repositorio Institucional de la UCLMinstname:Universidad de Castilla-La ManchaInglésinfo:eu-repo/semantics/openAccessoai:ruidera.uclm.es:10578/238392026-05-27T07:36:41Z
dc.title.none.fl_str_mv Serum proteome of dogs at subclinical and clinical onset of canine leishmaniosis
title Serum proteome of dogs at subclinical and clinical onset of canine leishmaniosis
spellingShingle Serum proteome of dogs at subclinical and clinical onset of canine leishmaniosis
Franco Martínez, Lorena
Biomarkers
Dog diseases
Leishmania
Proteomics
SWATH-MS
title_short Serum proteome of dogs at subclinical and clinical onset of canine leishmaniosis
title_full Serum proteome of dogs at subclinical and clinical onset of canine leishmaniosis
title_fullStr Serum proteome of dogs at subclinical and clinical onset of canine leishmaniosis
title_full_unstemmed Serum proteome of dogs at subclinical and clinical onset of canine leishmaniosis
title_sort Serum proteome of dogs at subclinical and clinical onset of canine leishmaniosis
dc.creator.none.fl_str_mv Franco Martínez, Lorena
Villar Rayo, Margarita María
Tvarijonaviciute, Asta
Escribano, Damíán
Bernal, Luis Jesús
Cerón Madrigal, José Joaquín
Thomas, María del Carmen
Mateos Hernández, Lourdes
Tecles Vicente, Fernando
Fuente García, José de Jesús de la
López López, Manuel Carlos
Martínez Subiela, Silvia
author Franco Martínez, Lorena
author_facet Franco Martínez, Lorena
Villar Rayo, Margarita María
Tvarijonaviciute, Asta
Escribano, Damíán
Bernal, Luis Jesús
Cerón Madrigal, José Joaquín
Thomas, María del Carmen
Mateos Hernández, Lourdes
Tecles Vicente, Fernando
Fuente García, José de Jesús de la
López López, Manuel Carlos
Martínez Subiela, Silvia
author_role author
author2 Villar Rayo, Margarita María
Tvarijonaviciute, Asta
Escribano, Damíán
Bernal, Luis Jesús
Cerón Madrigal, José Joaquín
Thomas, María del Carmen
Mateos Hernández, Lourdes
Tecles Vicente, Fernando
Fuente García, José de Jesús de la
López López, Manuel Carlos
Martínez Subiela, Silvia
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biomarkers
Dog diseases
Leishmania
Proteomics
SWATH-MS
topic Biomarkers
Dog diseases
Leishmania
Proteomics
SWATH-MS
description The objective of this study was to identify changes in serum proteome in dogs that may occur after an experimental infection at subclinical and clinical stages of canine leishmaniosis (CanL). For this purpose, canine pre- and post-infection with Leishmania infantum serum proteomes in the same dogs were analysed by a high-throughput label-based quantitative LC-MS/MS proteomic approach. A total of 169 proteins were identified, and 74 of them including complement C8 alpha chain, adiponectin, transferrin, sphingomyelin phosphodiesterase acid-like 3A and immunoglobulins showed different modulation between the different stages of CanL. These proteins could be considered as potential serum biomarkers of early diagnostic or disease progression in CanL. Additionally, biological pathways modulated during CanL such as blood coagulation or gonadotropin-releasing hormone receptor were revealed, which could help to understand the pathological mechanisms of the disease.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10578/23839
url https://hdl.handle.net/10578/23839
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Blackwell Verlag
publisher.none.fl_str_mv Blackwell Verlag
dc.source.none.fl_str_mv reponame:RUIdeRA. Repositorio Institucional de la UCLM
instname:Universidad de Castilla-La Mancha
instname_str Universidad de Castilla-La Mancha
reponame_str RUIdeRA. Repositorio Institucional de la UCLM
collection RUIdeRA. Repositorio Institucional de la UCLM
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869420483958538240
score 15.300719

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