NUCB2/nesfatin-1: a new adipokine expressed in human and murine chondrocytes with pro-inflammatory properties, an in vitro study

Nesfatin-1 is a recently discovered satiety-inducing adipokine identified in hypothalamic regions that regulates energy balance. So far, no data exist on NUCB2/nesfatin-1 localization in human and murine chondrocytes. Here, we therefore investigated NUCB2/nesfatin-1 gene and protein expression in hu...

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Autores: Scotece, Morena, Conde Aranda, Javier, Abella Cajigal, Vanesa, Rodríguez López, Verónica, Lago Paz, Francisca, Pino Mínguez, Jesús, Gómez-Reino Carnota, Juan Jesús, Gualillo ., Oreste
Tipo de recurso: artículo
Fecha de publicación:2014
País:España
Institución:Servizo Galego de Saúde (SERGAS)
Repositorio:RUNA. Repositorio da Consellería de Sanidade e Sergas
OAI Identifier:oai:runa.sergas.gal:20.500.11940/6543
Acceso en línea:http://hdl.handle.net/20.500.11940/6543
Access Level:acceso abierto
Palabra clave:Animals
Calcium-Binding Proteins
Cell Differentiation
Cell Line
Cells, Cultured
Chemokine CCL3
Chondrocytes
Cytokines
DNA-Binding Proteins
Humans
Interleukin-6
Interleukin-8
Mice
Nerve Tissue Proteins
Nucleobindins
RNA, Messenger
Nucb2
Nesfatin-1
chondrocytes
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oai_identifier_str oai:runa.sergas.gal:20.500.11940/6543
network_acronym_str ES
network_name_str España
repository_id_str
spelling NUCB2/nesfatin-1: a new adipokine expressed in human and murine chondrocytes with pro-inflammatory properties, an in vitro studyScotece, MorenaConde Aranda, JavierAbella Cajigal, VanesaRodríguez López, VerónicaLago Paz, FranciscaPino Mínguez, JesúsGómez-Reino Carnota, Juan JesúsGualillo ., OresteAnimalsCalcium-Binding ProteinsCell DifferentiationCell LineCells, CulturedChemokine CCL3ChondrocytesCytokinesDNA-Binding ProteinsHumansInterleukin-6Interleukin-8MiceNerve Tissue ProteinsNucleobindinsRNA, MessengerNucb2Nesfatin-1chondrocytesNesfatin-1 is a recently discovered satiety-inducing adipokine identified in hypothalamic regions that regulates energy balance. So far, no data exist on NUCB2/nesfatin-1 localization in human and murine chondrocytes. Here, we therefore investigated NUCB2/nesfatin-1 gene and protein expression in human and murine chondrocytes and the effect of nesfatin-1 on pro-inflammatory cytokines expression. Peptide localization was performed by laser confocal microscopy, NUCB2 mRNA expression was studied by RT-PCR and protein secretion was measured by XMap technology and Western blot analysis. First, we demonstrated cytoplasmic localization of NUCB2/nesfatin-1 peptide in both human and murine chondrocytes. We present evidence that both mRNA and protein expression of NUCB2 were increased during the differentiation of ATDC5 murine chondrocyte cell line. Furthermore, we demonstrated that nesfatin-1 induces IL-6 and MIP-1alpha mRNA expression and protein secretion in ATDC-5 cells challenged with IL-1, and also increases COX-2 mRNA expression in these cells. Finally, nesfatin-1 provoked a clear induction of pro-inflammatory agents, such as COX-2, IL-8, IL-6, and MIP-1alpha in human primary chondrocytes from OA patients.2014info:eu-repo/semantics/articlehttp://hdl.handle.net/20.500.11940/6543reponame:RUNA. Repositorio da Consellería de Sanidade e Sergasinstname:Servizo Galego de Saúde (SERGAS)Inglésinfo:eu-repo/semantics/openAccessoai:runa.sergas.gal:20.500.11940/65432026-06-12T08:40:47Z
dc.title.none.fl_str_mv NUCB2/nesfatin-1: a new adipokine expressed in human and murine chondrocytes with pro-inflammatory properties, an in vitro study
title NUCB2/nesfatin-1: a new adipokine expressed in human and murine chondrocytes with pro-inflammatory properties, an in vitro study
spellingShingle NUCB2/nesfatin-1: a new adipokine expressed in human and murine chondrocytes with pro-inflammatory properties, an in vitro study
Scotece, Morena
Animals
Calcium-Binding Proteins
Cell Differentiation
Cell Line
Cells, Cultured
Chemokine CCL3
Chondrocytes
Cytokines
DNA-Binding Proteins
Humans
Interleukin-6
Interleukin-8
Mice
Nerve Tissue Proteins
Nucleobindins
RNA, Messenger
Nucb2
Nesfatin-1
chondrocytes
title_short NUCB2/nesfatin-1: a new adipokine expressed in human and murine chondrocytes with pro-inflammatory properties, an in vitro study
title_full NUCB2/nesfatin-1: a new adipokine expressed in human and murine chondrocytes with pro-inflammatory properties, an in vitro study
title_fullStr NUCB2/nesfatin-1: a new adipokine expressed in human and murine chondrocytes with pro-inflammatory properties, an in vitro study
title_full_unstemmed NUCB2/nesfatin-1: a new adipokine expressed in human and murine chondrocytes with pro-inflammatory properties, an in vitro study
title_sort NUCB2/nesfatin-1: a new adipokine expressed in human and murine chondrocytes with pro-inflammatory properties, an in vitro study
dc.creator.none.fl_str_mv Scotece, Morena
Conde Aranda, Javier
Abella Cajigal, Vanesa
Rodríguez López, Verónica
Lago Paz, Francisca
Pino Mínguez, Jesús
Gómez-Reino Carnota, Juan Jesús
Gualillo ., Oreste
author Scotece, Morena
author_facet Scotece, Morena
Conde Aranda, Javier
Abella Cajigal, Vanesa
Rodríguez López, Verónica
Lago Paz, Francisca
Pino Mínguez, Jesús
Gómez-Reino Carnota, Juan Jesús
Gualillo ., Oreste
author_role author
author2 Conde Aranda, Javier
Abella Cajigal, Vanesa
Rodríguez López, Verónica
Lago Paz, Francisca
Pino Mínguez, Jesús
Gómez-Reino Carnota, Juan Jesús
Gualillo ., Oreste
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Animals
Calcium-Binding Proteins
Cell Differentiation
Cell Line
Cells, Cultured
Chemokine CCL3
Chondrocytes
Cytokines
DNA-Binding Proteins
Humans
Interleukin-6
Interleukin-8
Mice
Nerve Tissue Proteins
Nucleobindins
RNA, Messenger
Nucb2
Nesfatin-1
chondrocytes
topic Animals
Calcium-Binding Proteins
Cell Differentiation
Cell Line
Cells, Cultured
Chemokine CCL3
Chondrocytes
Cytokines
DNA-Binding Proteins
Humans
Interleukin-6
Interleukin-8
Mice
Nerve Tissue Proteins
Nucleobindins
RNA, Messenger
Nucb2
Nesfatin-1
chondrocytes
description Nesfatin-1 is a recently discovered satiety-inducing adipokine identified in hypothalamic regions that regulates energy balance. So far, no data exist on NUCB2/nesfatin-1 localization in human and murine chondrocytes. Here, we therefore investigated NUCB2/nesfatin-1 gene and protein expression in human and murine chondrocytes and the effect of nesfatin-1 on pro-inflammatory cytokines expression. Peptide localization was performed by laser confocal microscopy, NUCB2 mRNA expression was studied by RT-PCR and protein secretion was measured by XMap technology and Western blot analysis. First, we demonstrated cytoplasmic localization of NUCB2/nesfatin-1 peptide in both human and murine chondrocytes. We present evidence that both mRNA and protein expression of NUCB2 were increased during the differentiation of ATDC5 murine chondrocyte cell line. Furthermore, we demonstrated that nesfatin-1 induces IL-6 and MIP-1alpha mRNA expression and protein secretion in ATDC-5 cells challenged with IL-1, and also increases COX-2 mRNA expression in these cells. Finally, nesfatin-1 provoked a clear induction of pro-inflammatory agents, such as COX-2, IL-8, IL-6, and MIP-1alpha in human primary chondrocytes from OA patients.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.11940/6543
url http://hdl.handle.net/20.500.11940/6543
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:RUNA. Repositorio da Consellería de Sanidade e Sergas
instname:Servizo Galego de Saúde (SERGAS)
instname_str Servizo Galego de Saúde (SERGAS)
reponame_str RUNA. Repositorio da Consellería de Sanidade e Sergas
collection RUNA. Repositorio da Consellería de Sanidade e Sergas
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869420479491604480
score 15.301603

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