Porcine reproductive and respiratory syndrome virus impacts on gut microbiome in a strain virulence‐dependent fashion

[EN] Porcine reproductive and respiratory syndrome (PRRS) is a viral disease defined by reproductive problems, respiratory distress and a negative impact on growth rate and general condition. Virulent PRRS virus (PRRSV) strains have emerged in the last years with evident knowledge gaps in their impa...

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Autores: Argüello Rodríguez, Héctor, Rodríguez Gómez, Irene Magdalena, Sánchez Carvajal, José María, Pallares, Francisco José, Díaz, Iván, Cabrera Rubio, Raúl, Crispie, Fiona, Cotter, Paul D., Mateu, Enric, Martín Valls, Gerard, Carrasco, Librado, Gómez Laguna, Jaime
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Universidad Rey Juan Carlos
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/18829
Acceso en línea:https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.13757
https://hdl.handle.net/10612/18829
Access Level:acceso abierto
Palabra clave:Sanidad animal
Porcine reproductive and respiratory syndrome
PRRSV
3109 Ciencias Veterinarias
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spelling Porcine reproductive and respiratory syndrome virus impacts on gut microbiome in a strain virulence‐dependent fashionArgüello Rodríguez, HéctorRodríguez Gómez, Irene MagdalenaSánchez Carvajal, José MaríaPallares, Francisco JoséDíaz, IvánCabrera Rubio, RaúlCrispie, FionaCotter, Paul D.Mateu, EnricMartín Valls, GerardCarrasco, LibradoGómez Laguna, JaimeSanidad animalPorcine reproductive and respiratory syndromePRRSV3109 Ciencias Veterinarias[EN] Porcine reproductive and respiratory syndrome (PRRS) is a viral disease defined by reproductive problems, respiratory distress and a negative impact on growth rate and general condition. Virulent PRRS virus (PRRSV) strains have emerged in the last years with evident knowledge gaps in their impact on the host immune response. Thus, the present study examines the impact of acute PRRS virus (PRRSV) infection, with two strains of different virulence, on selected immune parameters and on the gut microbiota composition of infected pigs using 16S rRNA compositional sequencing. Pigs were infected with a low virulent (PRRS_3249) or a virulent (Lena) PRRSV-1 strain and euthanized at 1, 3, 6, 8 or 13 days post-inoculation (dpi). Faeces were collected from each animal at the necropsy time-point. Alpha and beta diversity analyses demonstrated that infection, particularly with the Lena strain, impacted the microbiome composition from 6 dpi onwards. Taxonomic differences revealed that infected pigs had higher abundance of Treponema and Methanobrevibacter (FDR < 0.05). Differences were more considerable for Lena- than for PRRS_3249-infected pigs, showing the impact of strain virulence in the intestinal changes. Lena-infected pigs had reduced abundancies of anaerobic commensals such as Roseburia, Anaerostipes, Butyricicoccus and Prevotella (P < 0.05). The depletion of these desirable commensals was significantly correlated to infection severity measured by viraemia, clinical signs, lung lesions and immune parameters (IL-6, IFN-γ and Hp serum levels). Altogether, the results from this study demonstrate the indirect impact of PRRSV infection on gut microbiome composition in a strain virulence-dependent fashion and its association with selected immune markersSIJ. G omez-Laguna is supported by a ‘Ram on y Cajal’ contract of the Spanish Ministry of Economy and Competitiveness (RYC-2014-16735). Hector Arg€uello is supported by the ‘Beatriz Galindo’ Programme from the Spanish Ministry of Education (BEAGAL-18-106). This work was partially supported by the Spanish Ministry of Education and Science (AGL2016-76111-R). Research in the Cotter laboratory is funded by Science Foundation Ireland in the form of a centre grants (APC Microbiome Ireland, Grant Number SFI/12/RC/2273, and Vistamilk, Grant Number SFI/16/RC/3835) and by the European Commission under the Horizon 2020 programme under grant number 818368 (MASTER)WileySanidad AnimalFacultad de Veterinaria2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.13757https://hdl.handle.net/10612/18829reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad Rey Juan CarlosInglésinfo:eu-repo/grantAgreement/MINECO/ Programa Estatal de Promoción del Talento y su Empleabilidad/ RYC-2014-16735http://creativecommons.org/licenses/by-nd/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/188292026-06-24T12:43:27Z
dc.title.none.fl_str_mv Porcine reproductive and respiratory syndrome virus impacts on gut microbiome in a strain virulence‐dependent fashion
title Porcine reproductive and respiratory syndrome virus impacts on gut microbiome in a strain virulence‐dependent fashion
spellingShingle Porcine reproductive and respiratory syndrome virus impacts on gut microbiome in a strain virulence‐dependent fashion
Argüello Rodríguez, Héctor
Sanidad animal
Porcine reproductive and respiratory syndrome
PRRSV
3109 Ciencias Veterinarias
title_short Porcine reproductive and respiratory syndrome virus impacts on gut microbiome in a strain virulence‐dependent fashion
title_full Porcine reproductive and respiratory syndrome virus impacts on gut microbiome in a strain virulence‐dependent fashion
title_fullStr Porcine reproductive and respiratory syndrome virus impacts on gut microbiome in a strain virulence‐dependent fashion
title_full_unstemmed Porcine reproductive and respiratory syndrome virus impacts on gut microbiome in a strain virulence‐dependent fashion
title_sort Porcine reproductive and respiratory syndrome virus impacts on gut microbiome in a strain virulence‐dependent fashion
dc.creator.none.fl_str_mv Argüello Rodríguez, Héctor
Rodríguez Gómez, Irene Magdalena
Sánchez Carvajal, José María
Pallares, Francisco José
Díaz, Iván
Cabrera Rubio, Raúl
Crispie, Fiona
Cotter, Paul D.
Mateu, Enric
Martín Valls, Gerard
Carrasco, Librado
Gómez Laguna, Jaime
author Argüello Rodríguez, Héctor
author_facet Argüello Rodríguez, Héctor
Rodríguez Gómez, Irene Magdalena
Sánchez Carvajal, José María
Pallares, Francisco José
Díaz, Iván
Cabrera Rubio, Raúl
Crispie, Fiona
Cotter, Paul D.
Mateu, Enric
Martín Valls, Gerard
Carrasco, Librado
Gómez Laguna, Jaime
author_role author
author2 Rodríguez Gómez, Irene Magdalena
Sánchez Carvajal, José María
Pallares, Francisco José
Díaz, Iván
Cabrera Rubio, Raúl
Crispie, Fiona
Cotter, Paul D.
Mateu, Enric
Martín Valls, Gerard
Carrasco, Librado
Gómez Laguna, Jaime
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Sanidad Animal
Facultad de Veterinaria
dc.subject.none.fl_str_mv Sanidad animal
Porcine reproductive and respiratory syndrome
PRRSV
3109 Ciencias Veterinarias
topic Sanidad animal
Porcine reproductive and respiratory syndrome
PRRSV
3109 Ciencias Veterinarias
description [EN] Porcine reproductive and respiratory syndrome (PRRS) is a viral disease defined by reproductive problems, respiratory distress and a negative impact on growth rate and general condition. Virulent PRRS virus (PRRSV) strains have emerged in the last years with evident knowledge gaps in their impact on the host immune response. Thus, the present study examines the impact of acute PRRS virus (PRRSV) infection, with two strains of different virulence, on selected immune parameters and on the gut microbiota composition of infected pigs using 16S rRNA compositional sequencing. Pigs were infected with a low virulent (PRRS_3249) or a virulent (Lena) PRRSV-1 strain and euthanized at 1, 3, 6, 8 or 13 days post-inoculation (dpi). Faeces were collected from each animal at the necropsy time-point. Alpha and beta diversity analyses demonstrated that infection, particularly with the Lena strain, impacted the microbiome composition from 6 dpi onwards. Taxonomic differences revealed that infected pigs had higher abundance of Treponema and Methanobrevibacter (FDR < 0.05). Differences were more considerable for Lena- than for PRRS_3249-infected pigs, showing the impact of strain virulence in the intestinal changes. Lena-infected pigs had reduced abundancies of anaerobic commensals such as Roseburia, Anaerostipes, Butyricicoccus and Prevotella (P < 0.05). The depletion of these desirable commensals was significantly correlated to infection severity measured by viraemia, clinical signs, lung lesions and immune parameters (IL-6, IFN-γ and Hp serum levels). Altogether, the results from this study demonstrate the indirect impact of PRRSV infection on gut microbiome composition in a strain virulence-dependent fashion and its association with selected immune markers
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.13757
https://hdl.handle.net/10612/18829
url https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.13757
https://hdl.handle.net/10612/18829
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO/ Programa Estatal de Promoción del Talento y su Empleabilidad/ RYC-2014-16735
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad Rey Juan Carlos
instname_str Universidad Rey Juan Carlos
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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