Circulating microRNA profile as a potential biomarker for obstructive sleep apnea diagnosis

Evaluation of microRNAs (miRNAs) could allow characterization of the obstructive sleep apnea (OSA) and help diagnose it more accurately. We aimed to examine circulating miRNA profiles to establish the differences between non-OSA and OSA patients. Additionally, we aimed to analyse the effect of conti...

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Autores: Santamaria-Martos, Fernando, Benítez, Iván, Ortega Delgado, Francisco José, Zapater, Andrea, Giron, Cristina, Pinilla, Lucía, Pacual, Lydia, Cortijo, Anunciación, Dalmases, Mireia, Fernández Real, José Manuel, Barbé Illa, Ferran, Sánchez de la Torre, Manuel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Universitat de Lleida (UdL)
Repositorio:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/68181
Acceso en línea:https://doi.org/10.1038/s41598-019-49940-1
http://hdl.handle.net/10459.1/68181
Access Level:acceso abierto
Palabra clave:MicroARN
Marcadors bioquímics
Síndromes d'apnea del son
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spelling Circulating microRNA profile as a potential biomarker for obstructive sleep apnea diagnosisSantamaria-Martos, FernandoBenítez, IvánOrtega Delgado, Francisco JoséZapater, AndreaGiron, CristinaPinilla, LucíaPacual, LydiaCortijo, AnunciaciónDalmases, MireiaFernández Real, José ManuelBarbé Illa, FerranSánchez de la Torre, ManuelMicroARNMarcadors bioquímicsSíndromes d'apnea del sonEvaluation of microRNAs (miRNAs) could allow characterization of the obstructive sleep apnea (OSA) and help diagnose it more accurately. We aimed to examine circulating miRNA profiles to establish the differences between non-OSA and OSA patients. Additionally, we aimed to analyse the effect of continuous positive airway pressure (CPAP) treatment on the miRNA profile. This observational, longitudinal study included 230 subjects referred to the Sleep Unit due to suspected OSA. Expression profiling of 188 miRNAs in plasma was performed in 27 subjects by TaqMan-Low-Density-Array. OSA-related miRNAs were selected for validation by RT-qPCR in 203 patients. Prediction models were built to discriminate between non-OSA and OSA: 1) NoSAS-score, 2) differentially expressed miRNAs, and 3) combination of NoSAS-score plus miRNAs. The differentially expressed miRNAs were measured after 6 months of follow-up. From the 14 miRNAs selected for validation, 6 were confirmed to be differentially expressed. The areas under the curve were 0.73 for the NoSAS-score, 0.81 for the miRNAs and 0.86 for the combination. After 6 months of CPAP treatment, miRNA levels in the OSA group seem to approximate to non-OSA levels. A cluster of miRNAs was identified to differentiate between non-OSA and OSA patients. CPAP treatment was associated with changes in the circulating miRNA profile.The founding sources have no role in the writing, data collection, analysis or interpretation of the study. The project is supported by PI 14/01266 and PI 18/00449 from the Instituto de Salud Carlos III (ISCIII), Fondo Europeo de Desarrollo Regional (FEDER), Sociedad Española de Neumología y Cirugía Torácica (SEPAR) and Societat Catalana de Pneumologia (SOCAP). The work is supported by IRBLleida Biobank (B.0000682) and PLATAFORMA BIOBANCOS PT17/0015/0027”. F.S.-M. is the recipient of a predoctoral fellowship from “AGAUR-University of Lleida” and Convocatòria d’Ajuts 2018 de Promoció a la Recerca en SalutNature Publishing Group2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://doi.org/10.1038/s41598-019-49940-1http://hdl.handle.net/10459.1/68181reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)InglésReproducció del document publicat a https://doi.org/10.1038/s41598-019-49940-1Scientific Reports, 2019, vol. 9, núm. 13456cc-by, (c) Santamaria-Martos et al., 2019info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/oai:repositori.udl.cat:10459.1/681812026-06-24T12:42:17Z
dc.title.none.fl_str_mv Circulating microRNA profile as a potential biomarker for obstructive sleep apnea diagnosis
title Circulating microRNA profile as a potential biomarker for obstructive sleep apnea diagnosis
spellingShingle Circulating microRNA profile as a potential biomarker for obstructive sleep apnea diagnosis
Santamaria-Martos, Fernando
MicroARN
Marcadors bioquímics
Síndromes d'apnea del son
title_short Circulating microRNA profile as a potential biomarker for obstructive sleep apnea diagnosis
title_full Circulating microRNA profile as a potential biomarker for obstructive sleep apnea diagnosis
title_fullStr Circulating microRNA profile as a potential biomarker for obstructive sleep apnea diagnosis
title_full_unstemmed Circulating microRNA profile as a potential biomarker for obstructive sleep apnea diagnosis
title_sort Circulating microRNA profile as a potential biomarker for obstructive sleep apnea diagnosis
dc.creator.none.fl_str_mv Santamaria-Martos, Fernando
Benítez, Iván
Ortega Delgado, Francisco José
Zapater, Andrea
Giron, Cristina
Pinilla, Lucía
Pacual, Lydia
Cortijo, Anunciación
Dalmases, Mireia
Fernández Real, José Manuel
Barbé Illa, Ferran
Sánchez de la Torre, Manuel
author Santamaria-Martos, Fernando
author_facet Santamaria-Martos, Fernando
Benítez, Iván
Ortega Delgado, Francisco José
Zapater, Andrea
Giron, Cristina
Pinilla, Lucía
Pacual, Lydia
Cortijo, Anunciación
Dalmases, Mireia
Fernández Real, José Manuel
Barbé Illa, Ferran
Sánchez de la Torre, Manuel
author_role author
author2 Benítez, Iván
Ortega Delgado, Francisco José
Zapater, Andrea
Giron, Cristina
Pinilla, Lucía
Pacual, Lydia
Cortijo, Anunciación
Dalmases, Mireia
Fernández Real, José Manuel
Barbé Illa, Ferran
Sánchez de la Torre, Manuel
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv MicroARN
Marcadors bioquímics
Síndromes d'apnea del son
topic MicroARN
Marcadors bioquímics
Síndromes d'apnea del son
description Evaluation of microRNAs (miRNAs) could allow characterization of the obstructive sleep apnea (OSA) and help diagnose it more accurately. We aimed to examine circulating miRNA profiles to establish the differences between non-OSA and OSA patients. Additionally, we aimed to analyse the effect of continuous positive airway pressure (CPAP) treatment on the miRNA profile. This observational, longitudinal study included 230 subjects referred to the Sleep Unit due to suspected OSA. Expression profiling of 188 miRNAs in plasma was performed in 27 subjects by TaqMan-Low-Density-Array. OSA-related miRNAs were selected for validation by RT-qPCR in 203 patients. Prediction models were built to discriminate between non-OSA and OSA: 1) NoSAS-score, 2) differentially expressed miRNAs, and 3) combination of NoSAS-score plus miRNAs. The differentially expressed miRNAs were measured after 6 months of follow-up. From the 14 miRNAs selected for validation, 6 were confirmed to be differentially expressed. The areas under the curve were 0.73 for the NoSAS-score, 0.81 for the miRNAs and 0.86 for the combination. After 6 months of CPAP treatment, miRNA levels in the OSA group seem to approximate to non-OSA levels. A cluster of miRNAs was identified to differentiate between non-OSA and OSA patients. CPAP treatment was associated with changes in the circulating miRNA profile.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1038/s41598-019-49940-1
http://hdl.handle.net/10459.1/68181
url https://doi.org/10.1038/s41598-019-49940-1
http://hdl.handle.net/10459.1/68181
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a https://doi.org/10.1038/s41598-019-49940-1
Scientific Reports, 2019, vol. 9, núm. 13456
dc.rights.none.fl_str_mv cc-by, (c) Santamaria-Martos et al., 2019
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv cc-by, (c) Santamaria-Martos et al., 2019
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universitat de Lleida (UdL)
instname_str Universitat de Lleida (UdL)
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
repository.name.fl_str_mv
repository.mail.fl_str_mv
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