African swine fever virus protein p30 interaction with heterogeneous nuclear ribonucleoprotein K (hnRNP-K) during infection
Heterogeneous nuclear ribonucleoprotein K (hnRNP-K) was identified as interacting cellular protein with the abundant immediate early protein p30 from African swine fever virus (ASFV) in a macrophage cDNA library screening. The interacting regions of hnRNP-K with p30 were established within residues...
| Autores: | , , |
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| Formato: | artículo |
| Fecha de publicación: | 2008 |
| País: | España |
| Recursos: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/289712 |
| Acesso em linha: | http://hdl.handle.net/10261/289712 |
| Access Level: | acceso abierto |
| Palavra-chave: | African swine fever virus p30 Ribonucleoproteins Virus-cell interaction |
| Resumo: | Heterogeneous nuclear ribonucleoprotein K (hnRNP-K) was identified as interacting cellular protein with the abundant immediate early protein p30 from African swine fever virus (ASFV) in a macrophage cDNA library screening. The interacting regions of hnRNP-K with p30 were established within residues 35-197, which represent KH1 and KH2 domains responsible for RNA binding. Colocalization of hnRNP-K and p30 was observed mainly in the nucleus, but not in the cytoplasm of infected cells and infection modified hnRNP-K subcellular distribution and decreased the incorporation of 5-fluorouridine into nascent RNA. Since similar effects were observed in cells transiently expressing p30, this interaction provides new insights into p30 function and could represent a possible additional mechanism by which ASFV downregulates host cell mRNA translation. Structured summary MINT-6742660hnRNP-K (uniprotkbP61978) physically interacts (MI0218)with p30 (uniprotkbQ8V1E7) by pull down (MI0096)MINT-6742673, MINT-6742696, MINT-6742729hnRNP-K (uniprotkb:P61978) physically interacts (MI:0218)with p30 (uniprotkb:Q8V1E7) by two hybrid (MI:0018)MINT-6742711:p30 (uniprotkb:Q8V1E7) and hnRNP-K (uniprotkb:P61978) colocalize (MI:0403) by fluorescence microscopy (MI:0416). © 2008 Federation of European Biochemical Societies. |
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