Genomic analysis of 40 prophages located in the genomes of 16 carbapenemase-producing clinical strains of Klebsiella pneumoniae

Klebsiella pneumoniae is the clinically most important species within the genus and, as a result of the continuous emergence of multi-drug resistant (MDR) strains, the cause of severe nosocomial infections. The decline in the effectiveness of antibiotic treatments for infections caused by MDR bacter...

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Detalles Bibliográficos
Autores: Bleriot, Ines, Trastoy, Rocío|||0000-0003-4580-7126, Blasco, Lucía|||0000-0002-4039-4142, Fernández-Cuenca, Felipe, Ambroa, Antón|||0000-0002-3525-2553, Fernández-García, Laura|||0000-0002-8531-6105, Pacios, Olga|||0000-0002-4476-856X, Perez-Nadales, Elena, Torre-Cisneros, J.|||0000-0003-1529-6302, Oteo-Iglesias, Jesús|||0000-0003-3327-8263, Navarro Risueño, Ferran|||0000-0002-4302-2838, Miró, Elisenda|||0000-0003-2781-6926, Pascual Hernández, Álvaro|||0000-0002-8672-5891, Bou, German|||0000-0001-8837-0062, Martínez-Martínez, Luis, Tomas, Maria|||0000-0003-4501-0387
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:230731
Acceso en línea:https://ddd.uab.cat/record/230731
https://dx.doi.org/urn:doi:10.1099/mgen.0.000369
Access Level:acceso abierto
Palabra clave:Klebsiella pneumoniae
Prophages
Bioinformatics
Genomic analysis
Comparative genomics
Phylogeny
Descripción
Sumario:Klebsiella pneumoniae is the clinically most important species within the genus and, as a result of the continuous emergence of multi-drug resistant (MDR) strains, the cause of severe nosocomial infections. The decline in the effectiveness of antibiotic treatments for infections caused by MDR bacteria has generated particular interest in the study of bacteriophages. In this study, we characterized a total of 40 temperate bacteriophages (prophages) with a genome range of 11.454-84.199 kb, predicted from 16 carbapenemase-producing clinical strains of belonging to different sequence types, previously identified by multilocus sequence typing. These prophages were grouped into the three families in the order Caudovirales (27 prophages belonging to the family Myoviridae, 10 prophages belonging to the family Siphoviridae and 3 prophages belonging to the family Podoviridae). Genomic comparison of the 40 prophage genomes led to the identification of four prophages isolated from different strains and of genome sizes of around 33.3, 36.1, 39.6 and 42.6 kb. These prophages showed sequence similarities (query cover.

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