Bacterial secretion of histamine within the gut influences immune responses within the lung
Background: Histamine is an important immunomodulator influencing both the innate and adaptive immune system. Certain host cells express the histidine decarboxylase enzyme (HDC), which is responsible for catalysing the decarboxylation of histidine to histamine. We and others have shown that bacteria...
| Autores: | , , , , , , , , , , , , , , |
|---|---|
| Tipo de documento: | artigo |
| Data de publicação: | 2018 |
| País: | España |
| Recursos: | Universidad de Navarra |
| Repositório: | Dadun. Depósito Académico Digital de la Universidad de Navarra |
| Idioma: | inglês |
| OAI Identifier: | oai:dadun.unav.edu:10171/68745 |
| Acesso em linha: | https://hdl.handle.net/10171/68745 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Gut-lung Histamine Inflammation Morganella morganii OVA mouse model |
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Bacterial secretion of histamine within the gut influences immune responses within the lungSabaté-Brescó, M. (Marina)|||/items/0f5a7fbd-3029-4fc2-be8f-7fd4ac39f3d1Barcik, W. (Weronika)|||/items/29925f80-364f-42c0-859a-95f3c115927bPugin, B. (Benoit)|||/items/4c726d36-fddb-41ea-9471-25f0d65374cdWestermann, P. (Patrick)|||/items/824169d4-0d5c-4c59-90a6-02ae66639c99Rinaldi, A. (Arturo)|||/items/5024dcdb-7253-42aa-b91a-842f679b0c3fGroeger, D. (David)|||/items/d5bb9a0f-60f9-4bf8-ae2f-c8375d8c37e8Van-Elst, D. (Dries)|||/items/6a2c43da-10ad-4192-940f-baa4801f9cf4Sokolowska, M. (Milena)|||/items/d42403a3-e907-470c-a107-6bacecf48ec9Krawczyk, K. (Krzysztof)|||/items/cd2f86c0-1923-4a31-8449-6f55357fea08Frei, R. (Remo)|||/items/0ef9d73c-8bff-48ed-880c-a3ad9676b704Ferstl, R. (Ruth)|||/items/35ebe8c7-094d-46de-a997-0e0c433f6b29Wawrzyniak, (Marcin)|||/items/96dd3be1-0bb0-4324-a17a-9862584f800bAltunbulakli, C. (Can)|||/items/da29b940-d13f-4d51-a48b-2f4c4c24ac4eAkdis, C. A. (Cezmi A.)|||/items/50d2978a-5a71-4f77-bd52-c438846667fbO'Mahony, L. (Liam)|||/items/53ef09c0-aa62-4b79-b34a-9deec1c5a9b7Gut-lungHistamineInflammationMorganella morganiiOVA mouse modelBackground: Histamine is an important immunomodulator influencing both the innate and adaptive immune system. Certain host cells express the histidine decarboxylase enzyme (HDC), which is responsible for catalysing the decarboxylation of histidine to histamine. We and others have shown that bacterial strains can also express HDC and secrete histamine; however, the influence of bacterial-derived histamine on the host immune responses distant to the gut is unclear. Methods: The Escherichia coli BL21 (E coli BL21) strain was genetically modified to express the Morganella morganii (M morganii)-derived HDC gene (E coli BL21_HTW). E coli BL21 and E coli BL21_HTW were gavaged to ovalbumin (OVA) sensitized and challenged mice to investigate the effect of bacterial-derived histamine on lung inflammatory responses. Results: Oral administration of E coli BL21_HTW, which is able to secrete histamine, to wild-type mice reduced lung eosinophilia and suppressed ex vivo OVA-stimulated cytokine secretion from lung cells in the OVA respiratory inflammation mouse model. In histamine receptor 2 (H2R)-deficient mice, administration of histamine-secreting bacteria also reduced inflammatory cell numbers in bronchoalveolar lavage (BAL). However, the suppressive effect of bacterial-derived histamine on BAL inflammation was lost in HDC-deficient mice. This loss of activity was associated with increased expression of histamine degrading enzymes and reduced histamine receptor expression. Conclusion: Histamine secretion from bacteria within the gut can have immunological consequences at distant mucosal sites, such as within the lung. These effects are influenced by host histamine receptor expression and the expression of histamine degrading enzymes.Dadun. Depósito Académico Digital Universidad de Navarra20242024-02-0520182018-01-0120182018-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/68745reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/687452026-06-21T12:47:57Z |
| dc.title.none.fl_str_mv |
Bacterial secretion of histamine within the gut influences immune responses within the lung |
| title |
Bacterial secretion of histamine within the gut influences immune responses within the lung |
| spellingShingle |
Bacterial secretion of histamine within the gut influences immune responses within the lung Sabaté-Brescó, M. (Marina)|||/items/0f5a7fbd-3029-4fc2-be8f-7fd4ac39f3d1 Gut-lung Histamine Inflammation Morganella morganii OVA mouse model |
| title_short |
Bacterial secretion of histamine within the gut influences immune responses within the lung |
| title_full |
Bacterial secretion of histamine within the gut influences immune responses within the lung |
| title_fullStr |
Bacterial secretion of histamine within the gut influences immune responses within the lung |
| title_full_unstemmed |
Bacterial secretion of histamine within the gut influences immune responses within the lung |
| title_sort |
Bacterial secretion of histamine within the gut influences immune responses within the lung |
| dc.creator.none.fl_str_mv |
Sabaté-Brescó, M. (Marina)|||/items/0f5a7fbd-3029-4fc2-be8f-7fd4ac39f3d1 Barcik, W. (Weronika)|||/items/29925f80-364f-42c0-859a-95f3c115927b Pugin, B. (Benoit)|||/items/4c726d36-fddb-41ea-9471-25f0d65374cd Westermann, P. (Patrick)|||/items/824169d4-0d5c-4c59-90a6-02ae66639c99 Rinaldi, A. (Arturo)|||/items/5024dcdb-7253-42aa-b91a-842f679b0c3f Groeger, D. (David)|||/items/d5bb9a0f-60f9-4bf8-ae2f-c8375d8c37e8 Van-Elst, D. (Dries)|||/items/6a2c43da-10ad-4192-940f-baa4801f9cf4 Sokolowska, M. (Milena)|||/items/d42403a3-e907-470c-a107-6bacecf48ec9 Krawczyk, K. (Krzysztof)|||/items/cd2f86c0-1923-4a31-8449-6f55357fea08 Frei, R. (Remo)|||/items/0ef9d73c-8bff-48ed-880c-a3ad9676b704 Ferstl, R. (Ruth)|||/items/35ebe8c7-094d-46de-a997-0e0c433f6b29 Wawrzyniak, (Marcin)|||/items/96dd3be1-0bb0-4324-a17a-9862584f800b Altunbulakli, C. (Can)|||/items/da29b940-d13f-4d51-a48b-2f4c4c24ac4e Akdis, C. A. (Cezmi A.)|||/items/50d2978a-5a71-4f77-bd52-c438846667fb O'Mahony, L. (Liam)|||/items/53ef09c0-aa62-4b79-b34a-9deec1c5a9b7 |
| author |
Sabaté-Brescó, M. (Marina)|||/items/0f5a7fbd-3029-4fc2-be8f-7fd4ac39f3d1 |
| author_facet |
Sabaté-Brescó, M. (Marina)|||/items/0f5a7fbd-3029-4fc2-be8f-7fd4ac39f3d1 Barcik, W. (Weronika)|||/items/29925f80-364f-42c0-859a-95f3c115927b Pugin, B. (Benoit)|||/items/4c726d36-fddb-41ea-9471-25f0d65374cd Westermann, P. (Patrick)|||/items/824169d4-0d5c-4c59-90a6-02ae66639c99 Rinaldi, A. (Arturo)|||/items/5024dcdb-7253-42aa-b91a-842f679b0c3f Groeger, D. (David)|||/items/d5bb9a0f-60f9-4bf8-ae2f-c8375d8c37e8 Van-Elst, D. (Dries)|||/items/6a2c43da-10ad-4192-940f-baa4801f9cf4 Sokolowska, M. (Milena)|||/items/d42403a3-e907-470c-a107-6bacecf48ec9 Krawczyk, K. (Krzysztof)|||/items/cd2f86c0-1923-4a31-8449-6f55357fea08 Frei, R. (Remo)|||/items/0ef9d73c-8bff-48ed-880c-a3ad9676b704 Ferstl, R. (Ruth)|||/items/35ebe8c7-094d-46de-a997-0e0c433f6b29 Wawrzyniak, (Marcin)|||/items/96dd3be1-0bb0-4324-a17a-9862584f800b Altunbulakli, C. (Can)|||/items/da29b940-d13f-4d51-a48b-2f4c4c24ac4e Akdis, C. A. (Cezmi A.)|||/items/50d2978a-5a71-4f77-bd52-c438846667fb O'Mahony, L. (Liam)|||/items/53ef09c0-aa62-4b79-b34a-9deec1c5a9b7 |
| author_role |
author |
| author2 |
Barcik, W. (Weronika)|||/items/29925f80-364f-42c0-859a-95f3c115927b Pugin, B. (Benoit)|||/items/4c726d36-fddb-41ea-9471-25f0d65374cd Westermann, P. (Patrick)|||/items/824169d4-0d5c-4c59-90a6-02ae66639c99 Rinaldi, A. (Arturo)|||/items/5024dcdb-7253-42aa-b91a-842f679b0c3f Groeger, D. (David)|||/items/d5bb9a0f-60f9-4bf8-ae2f-c8375d8c37e8 Van-Elst, D. (Dries)|||/items/6a2c43da-10ad-4192-940f-baa4801f9cf4 Sokolowska, M. (Milena)|||/items/d42403a3-e907-470c-a107-6bacecf48ec9 Krawczyk, K. (Krzysztof)|||/items/cd2f86c0-1923-4a31-8449-6f55357fea08 Frei, R. (Remo)|||/items/0ef9d73c-8bff-48ed-880c-a3ad9676b704 Ferstl, R. (Ruth)|||/items/35ebe8c7-094d-46de-a997-0e0c433f6b29 Wawrzyniak, (Marcin)|||/items/96dd3be1-0bb0-4324-a17a-9862584f800b Altunbulakli, C. (Can)|||/items/da29b940-d13f-4d51-a48b-2f4c4c24ac4e Akdis, C. A. (Cezmi A.)|||/items/50d2978a-5a71-4f77-bd52-c438846667fb O'Mahony, L. (Liam)|||/items/53ef09c0-aa62-4b79-b34a-9deec1c5a9b7 |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Dadun. Depósito Académico Digital Universidad de Navarra |
| dc.subject.none.fl_str_mv |
Gut-lung Histamine Inflammation Morganella morganii OVA mouse model |
| topic |
Gut-lung Histamine Inflammation Morganella morganii OVA mouse model |
| description |
Background: Histamine is an important immunomodulator influencing both the innate and adaptive immune system. Certain host cells express the histidine decarboxylase enzyme (HDC), which is responsible for catalysing the decarboxylation of histidine to histamine. We and others have shown that bacterial strains can also express HDC and secrete histamine; however, the influence of bacterial-derived histamine on the host immune responses distant to the gut is unclear. Methods: The Escherichia coli BL21 (E coli BL21) strain was genetically modified to express the Morganella morganii (M morganii)-derived HDC gene (E coli BL21_HTW). E coli BL21 and E coli BL21_HTW were gavaged to ovalbumin (OVA) sensitized and challenged mice to investigate the effect of bacterial-derived histamine on lung inflammatory responses. Results: Oral administration of E coli BL21_HTW, which is able to secrete histamine, to wild-type mice reduced lung eosinophilia and suppressed ex vivo OVA-stimulated cytokine secretion from lung cells in the OVA respiratory inflammation mouse model. In histamine receptor 2 (H2R)-deficient mice, administration of histamine-secreting bacteria also reduced inflammatory cell numbers in bronchoalveolar lavage (BAL). However, the suppressive effect of bacterial-derived histamine on BAL inflammation was lost in HDC-deficient mice. This loss of activity was associated with increased expression of histamine degrading enzymes and reduced histamine receptor expression. Conclusion: Histamine secretion from bacteria within the gut can have immunological consequences at distant mucosal sites, such as within the lung. These effects are influenced by host histamine receptor expression and the expression of histamine degrading enzymes. |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018 2018-01-01 2018 2018-01-01 2024 2024-02-05 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 |
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info:eu-repo/semantics/article |
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article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/10171/68745 |
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https://hdl.handle.net/10171/68745 |
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Inglés eng |
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Inglés |
| language |
eng |
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open access http://purl.org/coar/access_right/c_abf2 |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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application/pdf |
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reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra instname:Universidad de Navarra |
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Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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