Selective autophagy, lipophagy and mitophagy, in the Harderian gland along the oestrous cycle: a potential retrieval effect of melatonin

[EN]Sexual dimorphism has been reported in many processes. However, sexual bias in favour of the use of males is very present in science. One of the main reasons is that the impact of hormones in diverse pathways and processes such as autophagy have not been properly addressed in vivo. The Harderian...

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Autores: García Macia, Marina, Santos Ledo, Adrián, Caballero, Beatriz, Rubio-González, Adrian, de Luxán-Delgado, Beatriz, Potes, Yaiza, Rodríguez-González, Susana Mª., Boga, José Antonio, Coto-Montes, Ana
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/161912
Acceso en línea:http://hdl.handle.net/10366/161912
Access Level:acceso abierto
Palabra clave:Melatonin
Autophagy
Mouse
Lipophagy
Sexual cycle
Mitophagy
Harderian Gland
Sexual Behavior, Animal
Reproduction
Rodentia
2407 Biología Celular
2401 Biología Animal (Zoología)
2411.16 Fisiología de la Reproducción
glándula de Harder
reproducción
autofagia
conducta sexual animal
melatonina
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oai_identifier_str oai:gredos.usal.es:10366/161912
network_acronym_str ES
network_name_str España
repository_id_str
spelling Selective autophagy, lipophagy and mitophagy, in the Harderian gland along the oestrous cycle: a potential retrieval effect of melatoninGarcía Macia, MarinaSantos Ledo, AdriánCaballero, BeatrizRubio-González, Adriande Luxán-Delgado, BeatrizPotes, YaizaRodríguez-González, Susana Mª.Boga, José AntonioCoto-Montes, AnaMelatoninAutophagyMouseLipophagySexual cycleMitophagyHarderian GlandMelatoninSexual Behavior, AnimalReproductionRodentiaAutophagy2407 Biología Celular2401 Biología Animal (Zoología)2411.16 Fisiología de la Reproducciónglándula de HarderreproducciónRodentiaautofagiaconducta sexual animalmelatonina[EN]Sexual dimorphism has been reported in many processes. However, sexual bias in favour of the use of males is very present in science. One of the main reasons is that the impact of hormones in diverse pathways and processes such as autophagy have not been properly addressed in vivo. The Harderian gland is a perfect model to study autophagic modulation as it exhibits important changes during the oestrous cycle. The aim of this study is to identify the main processes behind Harderian gland differences under oestrous cycle and their modulator. In the present study we show that redox-sensitive transcription factors have an essential role: NF-κB may activate SQSTM1/p62 in oestrus, promoting selective types of autophagy: mitophagy and lipophagy. Nrf2 activation in dioestrus, leads the retrieval phase and restoration of mitochondrial homeostasis. Melatonin’s receptors show higher expression in dioestrus, leading to decreases in pro-inflammatory mediators and enhanced Nrf2 expression. Consequently, autophagy is blocked, and porphyrin release is reduced. All these results point to melatonin as one of the main modulators of the changes in autophagy during the oestrous cycle.We are members of the INPROTEOLYS, SEBBM, SEFAGIA and INEUROPA network. We acknowledge Fibrosis and Bolaños´lab for their support during the manuscript revisions. This work was supported by FISS-18-PI17/02009, C0120R3166, C0245R4032 and BH182173. MG-M has a postdoctoral fellowship from the Instituto de Salud Carlos III (ISCIII), Ministerio de Ciencia, Innovación y Universidades. Financial support from the University of Oviedo is also acknowledged.202520252019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10366/161912reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésFISS-18-PI17/02009C0120R3166C0245R4032BH182173Attribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:gredos.usal.es:10366/1619122026-06-07T06:28:51Z
dc.title.none.fl_str_mv Selective autophagy, lipophagy and mitophagy, in the Harderian gland along the oestrous cycle: a potential retrieval effect of melatonin
title Selective autophagy, lipophagy and mitophagy, in the Harderian gland along the oestrous cycle: a potential retrieval effect of melatonin
spellingShingle Selective autophagy, lipophagy and mitophagy, in the Harderian gland along the oestrous cycle: a potential retrieval effect of melatonin
García Macia, Marina
Melatonin
Autophagy
Mouse
Lipophagy
Sexual cycle
Mitophagy
Harderian Gland
Melatonin
Sexual Behavior, Animal
Reproduction
Rodentia
Autophagy
2407 Biología Celular
2401 Biología Animal (Zoología)
2411.16 Fisiología de la Reproducción
glándula de Harder
reproducción
Rodentia
autofagia
conducta sexual animal
melatonina
title_short Selective autophagy, lipophagy and mitophagy, in the Harderian gland along the oestrous cycle: a potential retrieval effect of melatonin
title_full Selective autophagy, lipophagy and mitophagy, in the Harderian gland along the oestrous cycle: a potential retrieval effect of melatonin
title_fullStr Selective autophagy, lipophagy and mitophagy, in the Harderian gland along the oestrous cycle: a potential retrieval effect of melatonin
title_full_unstemmed Selective autophagy, lipophagy and mitophagy, in the Harderian gland along the oestrous cycle: a potential retrieval effect of melatonin
title_sort Selective autophagy, lipophagy and mitophagy, in the Harderian gland along the oestrous cycle: a potential retrieval effect of melatonin
dc.creator.none.fl_str_mv García Macia, Marina
Santos Ledo, Adrián
Caballero, Beatriz
Rubio-González, Adrian
de Luxán-Delgado, Beatriz
Potes, Yaiza
Rodríguez-González, Susana Mª.
Boga, José Antonio
Coto-Montes, Ana
author García Macia, Marina
author_facet García Macia, Marina
Santos Ledo, Adrián
Caballero, Beatriz
Rubio-González, Adrian
de Luxán-Delgado, Beatriz
Potes, Yaiza
Rodríguez-González, Susana Mª.
Boga, José Antonio
Coto-Montes, Ana
author_role author
author2 Santos Ledo, Adrián
Caballero, Beatriz
Rubio-González, Adrian
de Luxán-Delgado, Beatriz
Potes, Yaiza
Rodríguez-González, Susana Mª.
Boga, José Antonio
Coto-Montes, Ana
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Melatonin
Autophagy
Mouse
Lipophagy
Sexual cycle
Mitophagy
Harderian Gland
Melatonin
Sexual Behavior, Animal
Reproduction
Rodentia
Autophagy
2407 Biología Celular
2401 Biología Animal (Zoología)
2411.16 Fisiología de la Reproducción
glándula de Harder
reproducción
Rodentia
autofagia
conducta sexual animal
melatonina
topic Melatonin
Autophagy
Mouse
Lipophagy
Sexual cycle
Mitophagy
Harderian Gland
Melatonin
Sexual Behavior, Animal
Reproduction
Rodentia
Autophagy
2407 Biología Celular
2401 Biología Animal (Zoología)
2411.16 Fisiología de la Reproducción
glándula de Harder
reproducción
Rodentia
autofagia
conducta sexual animal
melatonina
description [EN]Sexual dimorphism has been reported in many processes. However, sexual bias in favour of the use of males is very present in science. One of the main reasons is that the impact of hormones in diverse pathways and processes such as autophagy have not been properly addressed in vivo. The Harderian gland is a perfect model to study autophagic modulation as it exhibits important changes during the oestrous cycle. The aim of this study is to identify the main processes behind Harderian gland differences under oestrous cycle and their modulator. In the present study we show that redox-sensitive transcription factors have an essential role: NF-κB may activate SQSTM1/p62 in oestrus, promoting selective types of autophagy: mitophagy and lipophagy. Nrf2 activation in dioestrus, leads the retrieval phase and restoration of mitochondrial homeostasis. Melatonin’s receptors show higher expression in dioestrus, leading to decreases in pro-inflammatory mediators and enhanced Nrf2 expression. Consequently, autophagy is blocked, and porphyrin release is reduced. All these results point to melatonin as one of the main modulators of the changes in autophagy during the oestrous cycle.
publishDate 2019
dc.date.none.fl_str_mv 2019
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10366/161912
url http://hdl.handle.net/10366/161912
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv FISS-18-PI17/02009
C0120R3166
C0245R4032
BH182173
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca
instname:Universidad de Salamanca (USAL)
instname_str Universidad de Salamanca (USAL)
reponame_str GREDOS. Repositorio Institucional de la Universidad de Salamanca
collection GREDOS. Repositorio Institucional de la Universidad de Salamanca
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429