Effect of formic acid treatment on carbon and nitrogen stable isotope ratios in sperm whale teeth dentine

Rationale: Stable isotope analysis of growth layers in sperm whale teeth dentine can provide valuable insight into individual long-distance displacements and diet. Although treating teeth half-sections with formic acid and rubbing their surface with a graphite pencil improves growth layer visibility...

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Autores: Stukonytė, Laura, Borrell Thió, Assumpció, Drago, Massimiliano, Lockyer, Christina, Víkingsson, Gísli, Aguilar, Àlex
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/219729
Acceso en línea:https://hdl.handle.net/2445/219729
Access Level:acceso abierto
Palabra clave:Balenes
Isòtops estables en ecologia
Dentina
Whales
Stable isotopes in ecological research
Dentin
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spelling Effect of formic acid treatment on carbon and nitrogen stable isotope ratios in sperm whale teeth dentineStukonytė, LauraBorrell Thió, AssumpcióDrago, MassimilianoLockyer, ChristinaVíkingsson, GísliAguilar, ÀlexBalenesIsòtops estables en ecologiaDentinaWhalesStable isotopes in ecological researchDentinRationale: Stable isotope analysis of growth layers in sperm whale teeth dentine can provide valuable insight into individual long-distance displacements and diet. Although treating teeth half-sections with formic acid and rubbing their surface with a graphite pencil improves growth layer visibility and reduces sampling error, previous studies mostly used untreated half-sections as the effect that this treatment may have on stable isotope ratios in dentine is unknown. The present study investigates the treatment effect on stable C and N isotope ratios in sperm whale teeth dentine. Methods: In the teeth of 30 sperm whales, we analysed and compared samples of powdered dentine obtained from (a) untreated half-sections, (b) half-sections etched with formic acid and rubbed with a graphite pencil and (c) half-sections etched with formic acid from which the graphite pencil rubbing had been cleansed off. δ13 C and δ15 N values were compared between the three sample groups. Results: We found significant differences in values of both elements between untreated and etched samples, with a mean increase of 0.2% in δ13C and δ15N values in the etched samples. No significant differences were found between etched samples with graphite rubbing versus those without it. Significant linear regression models were calculated to predict untreated δ13C and δ15N values from the values obtained in the etched half-sections with limited precision. Conclusions: We show for the first time that formic acid etching has a clear effect on C and N values in sperm whale teeth dentine. The developed models permit the estimation of untreated values from etched half-sections, thus enabling the use of the latter in stable isotope analysis. However, as treatment procedures may vary between studies, it is advisable that similar predictive models are developed case-by-case to ensure comparability of results.Wiley2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/219729Articles publicats en revistes (Biologia Evolutiva, Ecologia i Ciències Ambientals)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1002/rcm.9500Rapid Communications in Mass Spectrometry, 2023, vol. 37, num.10, p. 1-7https://doi.org/10.1002/rcm.9500cc-by (c) Stukonytė, Laura et al., 2023http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/2197292026-05-27T06:46:51Z
dc.title.none.fl_str_mv Effect of formic acid treatment on carbon and nitrogen stable isotope ratios in sperm whale teeth dentine
title Effect of formic acid treatment on carbon and nitrogen stable isotope ratios in sperm whale teeth dentine
spellingShingle Effect of formic acid treatment on carbon and nitrogen stable isotope ratios in sperm whale teeth dentine
Stukonytė, Laura
Balenes
Isòtops estables en ecologia
Dentina
Whales
Stable isotopes in ecological research
Dentin
title_short Effect of formic acid treatment on carbon and nitrogen stable isotope ratios in sperm whale teeth dentine
title_full Effect of formic acid treatment on carbon and nitrogen stable isotope ratios in sperm whale teeth dentine
title_fullStr Effect of formic acid treatment on carbon and nitrogen stable isotope ratios in sperm whale teeth dentine
title_full_unstemmed Effect of formic acid treatment on carbon and nitrogen stable isotope ratios in sperm whale teeth dentine
title_sort Effect of formic acid treatment on carbon and nitrogen stable isotope ratios in sperm whale teeth dentine
dc.creator.none.fl_str_mv Stukonytė, Laura
Borrell Thió, Assumpció
Drago, Massimiliano
Lockyer, Christina
Víkingsson, Gísli
Aguilar, Àlex
author Stukonytė, Laura
author_facet Stukonytė, Laura
Borrell Thió, Assumpció
Drago, Massimiliano
Lockyer, Christina
Víkingsson, Gísli
Aguilar, Àlex
author_role author
author2 Borrell Thió, Assumpció
Drago, Massimiliano
Lockyer, Christina
Víkingsson, Gísli
Aguilar, Àlex
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Balenes
Isòtops estables en ecologia
Dentina
Whales
Stable isotopes in ecological research
Dentin
topic Balenes
Isòtops estables en ecologia
Dentina
Whales
Stable isotopes in ecological research
Dentin
description Rationale: Stable isotope analysis of growth layers in sperm whale teeth dentine can provide valuable insight into individual long-distance displacements and diet. Although treating teeth half-sections with formic acid and rubbing their surface with a graphite pencil improves growth layer visibility and reduces sampling error, previous studies mostly used untreated half-sections as the effect that this treatment may have on stable isotope ratios in dentine is unknown. The present study investigates the treatment effect on stable C and N isotope ratios in sperm whale teeth dentine. Methods: In the teeth of 30 sperm whales, we analysed and compared samples of powdered dentine obtained from (a) untreated half-sections, (b) half-sections etched with formic acid and rubbed with a graphite pencil and (c) half-sections etched with formic acid from which the graphite pencil rubbing had been cleansed off. δ13 C and δ15 N values were compared between the three sample groups. Results: We found significant differences in values of both elements between untreated and etched samples, with a mean increase of 0.2% in δ13C and δ15N values in the etched samples. No significant differences were found between etched samples with graphite rubbing versus those without it. Significant linear regression models were calculated to predict untreated δ13C and δ15N values from the values obtained in the etched half-sections with limited precision. Conclusions: We show for the first time that formic acid etching has a clear effect on C and N values in sperm whale teeth dentine. The developed models permit the estimation of untreated values from etched half-sections, thus enabling the use of the latter in stable isotope analysis. However, as treatment procedures may vary between studies, it is advisable that similar predictive models are developed case-by-case to ensure comparability of results.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/219729
url https://hdl.handle.net/2445/219729
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1002/rcm.9500
Rapid Communications in Mass Spectrometry, 2023, vol. 37, num.10, p. 1-7
https://doi.org/10.1002/rcm.9500
dc.rights.none.fl_str_mv cc-by (c) Stukonytė, Laura et al., 2023
http://creativecommons.org/licenses/by/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Stukonytė, Laura et al., 2023
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia Evolutiva, Ecologia i Ciències Ambientals)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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