Simple analytical approach to determine the microbiological profile of bee pollen by MALDI-TOF

Producción Científica

Detalles Bibliográficos
Autores: Prieto Diez, Estela, Ares Sacristán, Ana María, Bernal Yagüe, José Luis, Valverde Bastardo, Silvia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2026
País:España
Institución:Universidad de Valladolid
Repositorio:UVaDOC. Repositorio Documental de la Universidad de Valladolid
OAI Identifier:oai:uvadoc.uva.es:10324/82488
Acceso en línea:https://doi.org/10.1016/j.foodcont.2025.111939
https://uvadoc.uva.es/handle/10324/82488
Access Level:acceso abierto
Palabra clave:Bee pollen
Biotyper-MALDI-TOF
Food safety
Fungal protein extraction
Green metrics
Microbiological contamination
Microbiological profiling
23 Química
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spelling Simple analytical approach to determine the microbiological profile of bee pollen by MALDI-TOFPrieto Diez, EstelaAres Sacristán, Ana MaríaBernal Yagüe, José LuisValverde Bastardo, SilviaBee pollenBiotyper-MALDI-TOFFood safetyFungal protein extractionGreen metricsMicrobiological contaminationMicrobiological profiling23 QuímicaProducción CientíficaBee pollen is a natural food supplement collected by honeybees that has significant nutritional value and ther- apeutic qualities, including antioxidant, antibacterial, and anticarcinogenic properties. Due to its hygroscopic nature, bee pollen readily promotes microbial growth; however, there is no specific legislation for bee pollen in the European Union. Microbiological contamination therefore stands out as a crucial factor in determining its quality. To address this, the study focused on isolating bacterial and fungal colonies from bee pollen using various culture media, followed by a protein extraction based on a lysis process. Identification was achieved through the Biotyper-MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization coupled to Time-of-Flight de- tector) system. The greenness and blueness of the methods were assessed using different tools, which classified them as environmentally friendly and practical. Thirty-two samples were analyzed using proposed sample treatment approaches. Data were processed using chemometric techniques based on linear models. The results revealed the presence of both beneficial and potentially pathogenic species, including key representatives such as Bacillus licheniformis, Micrococcus luteus, and Aspergillus fumigatus. These findings highlight the importance of microbiota characterization in bee pollen and demonstrate that MALDI-TOF is a rapid and effective tool for profiling culturable microbial communities in this matrix.Ministerio de Ciencia e Innovación - MICIU/AEI750/10.13039/501100011033 y por FEDER, UE (grant PID2022-141679OR-C33)Elsevier2026info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://doi.org/10.1016/j.foodcont.2025.111939https://uvadoc.uva.es/handle/10324/82488reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolidinstname:Universidad de ValladolidIngléshttps://www.sciencedirect.com/science/article/pii/S0956713525008084info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/oai:uvadoc.uva.es:10324/824882026-06-13T12:44:47Z
dc.title.none.fl_str_mv Simple analytical approach to determine the microbiological profile of bee pollen by MALDI-TOF
title Simple analytical approach to determine the microbiological profile of bee pollen by MALDI-TOF
spellingShingle Simple analytical approach to determine the microbiological profile of bee pollen by MALDI-TOF
Prieto Diez, Estela
Bee pollen
Biotyper-MALDI-TOF
Food safety
Fungal protein extraction
Green metrics
Microbiological contamination
Microbiological profiling
23 Química
title_short Simple analytical approach to determine the microbiological profile of bee pollen by MALDI-TOF
title_full Simple analytical approach to determine the microbiological profile of bee pollen by MALDI-TOF
title_fullStr Simple analytical approach to determine the microbiological profile of bee pollen by MALDI-TOF
title_full_unstemmed Simple analytical approach to determine the microbiological profile of bee pollen by MALDI-TOF
title_sort Simple analytical approach to determine the microbiological profile of bee pollen by MALDI-TOF
dc.creator.none.fl_str_mv Prieto Diez, Estela
Ares Sacristán, Ana María
Bernal Yagüe, José Luis
Valverde Bastardo, Silvia
author Prieto Diez, Estela
author_facet Prieto Diez, Estela
Ares Sacristán, Ana María
Bernal Yagüe, José Luis
Valverde Bastardo, Silvia
author_role author
author2 Ares Sacristán, Ana María
Bernal Yagüe, José Luis
Valverde Bastardo, Silvia
author2_role author
author
author
dc.subject.none.fl_str_mv Bee pollen
Biotyper-MALDI-TOF
Food safety
Fungal protein extraction
Green metrics
Microbiological contamination
Microbiological profiling
23 Química
topic Bee pollen
Biotyper-MALDI-TOF
Food safety
Fungal protein extraction
Green metrics
Microbiological contamination
Microbiological profiling
23 Química
description Producción Científica
publishDate 2026
dc.date.none.fl_str_mv 2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.foodcont.2025.111939
https://uvadoc.uva.es/handle/10324/82488
url https://doi.org/10.1016/j.foodcont.2025.111939
https://uvadoc.uva.es/handle/10324/82488
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0956713525008084
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolid
instname:Universidad de Valladolid
instname_str Universidad de Valladolid
reponame_str UVaDOC. Repositorio Documental de la Universidad de Valladolid
collection UVaDOC. Repositorio Documental de la Universidad de Valladolid
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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