Exploring the pangenome of Mycoplasma hyorhinis in search of potential virulence markers

Mycoplasma hyorhinis (homotypic synonym of Mesomycoplasma hyorhinis) is a pathobiont from the upper respiratory tract of pigs. Under unclear circumstances, it can disseminate systemically and cause disease. Although some studies have reported different infectious capabilities among strains, no facto...

ver descrição completa

Detalhes bibliográficos
Autores: Obregon-Gutierrez, P, Nogales, J, Gonzalez-Torres, C, Huerta, E, Rubio, A, Domingo, M, Segales, J, Kochanowski, K, Perez-Pulido, AJ, Aragon, V, Correa-Fiz, F, Sibila, M
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Recursos:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
Repositorio:r-FISABIO. Repositorio Institucional de Producción Científica
OAI Identifier:oai:dnet:r-fisabio___::606c3b7667aa3214e24be7cb12b4af64
Acesso em linha:https://fisabio.portalinvestigacion.com/publicaciones/20940
Access Level:acceso abierto
Palavra-chave:Mycoplasma hyorhinis
Pangenome
Strain virulence
Lesions
Nasal cavity
id ES_d2a5fa97c9067e8f0b60e9690437aff0
oai_identifier_str oai:dnet:r-fisabio___::606c3b7667aa3214e24be7cb12b4af64
network_acronym_str ES
network_name_str España
repository_id_str
spelling Exploring the pangenome of Mycoplasma hyorhinis in search of potential virulence markersObregon-Gutierrez, PNogales, JGonzalez-Torres, CHuerta, ERubio, ADomingo, MSegales, JKochanowski, KPerez-Pulido, AJAragon, VCorrea-Fiz, FSibila, MMycoplasma hyorhinisPangenomeStrain virulenceLesionsNasal cavityMycoplasma hyorhinis (homotypic synonym of Mesomycoplasma hyorhinis) is a pathobiont from the upper respiratory tract of pigs. Under unclear circumstances, it can disseminate systemically and cause disease. Although some studies have reported different infectious capabilities among strains, no factors have been directly linked to virulence. This study aimed to analyze the core and accessory genes of all available M. hyorhinis strains (pangenome) to identify potential virulence markers. We characterized the pangenome of 110 strains, including isolates from healthy (nasal cavity) and diseased (systemic organs, nasal cavity or lung) animals. Comparative analyses were performed according to the clinical background. Although most putative virulence genes were shared, we identified several genes absent in most health-associated strains related to DNA-processing mechanisms, including hsdM-hsdR restriction-modification system and various helicases. Furthermore, the particular analysis of variable lipoprotein (vlp) genes revealed a similar presence in all strains but higher number of repeats in region III of vlpF in strains isolated from systemic lesions. Genome-scale metabolic models were used to infer the metabolic capabilities of the strains, showing highly conserved predicted reactomes, including growth capabilities and auxotrophies. In conclusion, although all strains may carry genes enabling disease, nasal strains from healthy animals lacked some DNA-processing genes and showed distinct vlp patterns. Additional genomes, especially from strains isolated from healthy animals, would be needed to confirm these findings.NATURE PORTFOLIO2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://fisabio.portalinvestigacion.com/publicaciones/20940Scientific ReportsISSN: 20452322reponame:r-FISABIO. Repositorio Institucional de Producción Científicainstname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)Inglésinfo:eu-repo/semantics/openAccessoai:dnet:r-fisabio___::606c3b7667aa3214e24be7cb12b4af642026-06-11T12:45:17Z
dc.title.none.fl_str_mv Exploring the pangenome of Mycoplasma hyorhinis in search of potential virulence markers
title Exploring the pangenome of Mycoplasma hyorhinis in search of potential virulence markers
spellingShingle Exploring the pangenome of Mycoplasma hyorhinis in search of potential virulence markers
Obregon-Gutierrez, P
Mycoplasma hyorhinis
Pangenome
Strain virulence
Lesions
Nasal cavity
title_short Exploring the pangenome of Mycoplasma hyorhinis in search of potential virulence markers
title_full Exploring the pangenome of Mycoplasma hyorhinis in search of potential virulence markers
title_fullStr Exploring the pangenome of Mycoplasma hyorhinis in search of potential virulence markers
title_full_unstemmed Exploring the pangenome of Mycoplasma hyorhinis in search of potential virulence markers
title_sort Exploring the pangenome of Mycoplasma hyorhinis in search of potential virulence markers
dc.creator.none.fl_str_mv Obregon-Gutierrez, P
Nogales, J
Gonzalez-Torres, C
Huerta, E
Rubio, A
Domingo, M
Segales, J
Kochanowski, K
Perez-Pulido, AJ
Aragon, V
Correa-Fiz, F
Sibila, M
author Obregon-Gutierrez, P
author_facet Obregon-Gutierrez, P
Nogales, J
Gonzalez-Torres, C
Huerta, E
Rubio, A
Domingo, M
Segales, J
Kochanowski, K
Perez-Pulido, AJ
Aragon, V
Correa-Fiz, F
Sibila, M
author_role author
author2 Nogales, J
Gonzalez-Torres, C
Huerta, E
Rubio, A
Domingo, M
Segales, J
Kochanowski, K
Perez-Pulido, AJ
Aragon, V
Correa-Fiz, F
Sibila, M
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Mycoplasma hyorhinis
Pangenome
Strain virulence
Lesions
Nasal cavity
topic Mycoplasma hyorhinis
Pangenome
Strain virulence
Lesions
Nasal cavity
description Mycoplasma hyorhinis (homotypic synonym of Mesomycoplasma hyorhinis) is a pathobiont from the upper respiratory tract of pigs. Under unclear circumstances, it can disseminate systemically and cause disease. Although some studies have reported different infectious capabilities among strains, no factors have been directly linked to virulence. This study aimed to analyze the core and accessory genes of all available M. hyorhinis strains (pangenome) to identify potential virulence markers. We characterized the pangenome of 110 strains, including isolates from healthy (nasal cavity) and diseased (systemic organs, nasal cavity or lung) animals. Comparative analyses were performed according to the clinical background. Although most putative virulence genes were shared, we identified several genes absent in most health-associated strains related to DNA-processing mechanisms, including hsdM-hsdR restriction-modification system and various helicases. Furthermore, the particular analysis of variable lipoprotein (vlp) genes revealed a similar presence in all strains but higher number of repeats in region III of vlpF in strains isolated from systemic lesions. Genome-scale metabolic models were used to infer the metabolic capabilities of the strains, showing highly conserved predicted reactomes, including growth capabilities and auxotrophies. In conclusion, although all strains may carry genes enabling disease, nasal strains from healthy animals lacked some DNA-processing genes and showed distinct vlp patterns. Additional genomes, especially from strains isolated from healthy animals, would be needed to confirm these findings.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://fisabio.portalinvestigacion.com/publicaciones/20940
url https://fisabio.portalinvestigacion.com/publicaciones/20940
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv NATURE PORTFOLIO
publisher.none.fl_str_mv NATURE PORTFOLIO
dc.source.none.fl_str_mv Scientific Reports
ISSN: 20452322
reponame:r-FISABIO. Repositorio Institucional de Producción Científica
instname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
instname_str Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
reponame_str r-FISABIO. Repositorio Institucional de Producción Científica
collection r-FISABIO. Repositorio Institucional de Producción Científica
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869420391012761600
score 15,811543

Registos relacionados