Respiration quotient variability: bacterial evidence

13 pages, 6 figures, 1 table

Detalles Bibliográficos
Autores: Romero-Kutzner, V., Packard, Theodore T., Berdalet, Elisa, Roy, Suzanne, Gagné, Jean-Pierre, Gómez, May
Tipo de recurso: artículo
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/117577
Acceso en línea:http://hdl.handle.net/10261/117577
Access Level:acceso abierto
Palabra clave:Growth
Potential respiration
ETS
Electron transport system
IDH
Isocitrate dehydrogenase
CO2 production
O2 consumption
O2
CO2
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spelling Respiration quotient variability: bacterial evidenceRomero-Kutzner, V.Packard, Theodore T.Berdalet, ElisaRoy, SuzanneGagné, Jean-PierreGómez, MayGrowthPotential respirationETSElectron transport systemIDHIsocitrate dehydrogenaseCO2 productionO2 consumptionO2CO213 pages, 6 figures, 1 tableRespiratory metabolism was compared between 2 different physiological states of acetate- and pyruvate-grown cultures of Pseudomonas nautica and Vibrio natriegens. Here, we analyze 35 h and 520 h experiments in which time-courses of protein, pyruvate, acetate, respiratory CO2 production (RCO2), respiratory O2 consumption (RO2), isocitrate dehydrogenase (IDH) activity, and potential respiration (Φ) were measured. Respiratory quotients (RQs) were calculated as the ratio of the respiration rates (RCO2/RO2). Such RQs are widely used in ocean ecosystem models, in calculations of carbon flux, and in evaluations of the ocean’s metabolic balance. In all the cultures, the RQ tended to increase. In the case of P. nautica on acetate, the RQ rose nearly an order of magnitude from values below 1 during carbon-substrate sufficiency to values close to 10 during carbon-substrate deficiency. In all the cultures, the respiration rates during the growth period paralleled the biomass increase, but after the substrates were exhausted, the respiration rates fell. In contrast, through this same transition period, the IDH activity and the Φ remained relatively high for the first 10 h of carbon-substrate deprivation, and then, these enzyme activities fell slowly, along with the biomass, as the carbon-substrate deprivation continued. The nutritional state of the bacteria affected the RQ, rendering the RQ variable for physiological and ecological purposes. These results argue that ecosystem models, oceanographic calculations of carbon flux, and evaluations of the ocean’s metabolic balance that are influenced by bacterial metabolism need to be reconsidered in light of RQ variabilityThis work was supported by BIOMBA project CTM 2012-32729/MAR granted to M.G., the University Foundation of Las Palmas by the program ‘Innova Canarias 2020’ financed by Endesa granted to V.R.-K., and a CIE Canarias tri-Continental Atlantic Campus (#CEI10/00018) contract to T.T.P.Peer ReviewedInter Research2015201520152015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/117577reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.3354/meps11062info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1175772026-05-22T06:33:51Z
dc.title.none.fl_str_mv Respiration quotient variability: bacterial evidence
title Respiration quotient variability: bacterial evidence
spellingShingle Respiration quotient variability: bacterial evidence
Romero-Kutzner, V.
Growth
Potential respiration
ETS
Electron transport system
IDH
Isocitrate dehydrogenase
CO2 production
O2 consumption
O2
CO2
title_short Respiration quotient variability: bacterial evidence
title_full Respiration quotient variability: bacterial evidence
title_fullStr Respiration quotient variability: bacterial evidence
title_full_unstemmed Respiration quotient variability: bacterial evidence
title_sort Respiration quotient variability: bacterial evidence
dc.creator.none.fl_str_mv Romero-Kutzner, V.
Packard, Theodore T.
Berdalet, Elisa
Roy, Suzanne
Gagné, Jean-Pierre
Gómez, May
author Romero-Kutzner, V.
author_facet Romero-Kutzner, V.
Packard, Theodore T.
Berdalet, Elisa
Roy, Suzanne
Gagné, Jean-Pierre
Gómez, May
author_role author
author2 Packard, Theodore T.
Berdalet, Elisa
Roy, Suzanne
Gagné, Jean-Pierre
Gómez, May
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Growth
Potential respiration
ETS
Electron transport system
IDH
Isocitrate dehydrogenase
CO2 production
O2 consumption
O2
CO2
topic Growth
Potential respiration
ETS
Electron transport system
IDH
Isocitrate dehydrogenase
CO2 production
O2 consumption
O2
CO2
description 13 pages, 6 figures, 1 table
publishDate 2015
dc.date.none.fl_str_mv 2015
2015
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/117577
url http://hdl.handle.net/10261/117577
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.3354/meps11062
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Inter Research
publisher.none.fl_str_mv Inter Research
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869420390946701312
score 15.812455

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