Time course of bilateral microglial activation in a mouse model of laser-induced glaucoma

Microglial activation is associated with glaucoma. In the model of unilateral laser-induced ocular hypertension (OHT), the time point at which the inflammatory process peaks remains unknown. Different time points (1, 3, 5, 8, and 15 d) were compared to analyze signs of microglial activation both in...

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Detalles Bibliográficos
Autores: Ramírez Sebastián, Ana Isabel, Hoz Montañana, María Rosa De, Fernández Albarral, José, García Martín, Elena Salobrar, Rojas López, María Blanca, Valiente Soriano, Francisco J., Avilés Trigueros, Marcelino, Villegas Pérez, María P., Vidal Sanz, Manuel, Triviño Casado, Alberto, Ramírez Sebastián, José Manuel, Salazar Corral, Juan José
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/103770
Acceso en línea:https://hdl.handle.net/20.500.14352/103770
Access Level:acceso abierto
Palabra clave:617.735
617.7-007.681
Animal disease models
Microglia
Retina
Oftalmología
3201.09 Oftalmología
Descripción
Sumario:Microglial activation is associated with glaucoma. In the model of unilateral laser-induced ocular hypertension (OHT), the time point at which the inflammatory process peaks remains unknown. Different time points (1, 3, 5, 8, and 15 d) were compared to analyze signs of microglial activation both in OHT and contralateral eyes. In both eyes, microglial activation was detected in all retinal layers at all time points analyzed, including: i) increase in the cell number in the outer segment photoreceptor layer and plexiform layers (only in OHT eyes) from 3 d onward; ii) increase in soma size from 1 d onward; iii) retraction of the processes from 1 d in OHT eyes and 3 d in contralateral eyes; iv) increase in the area of the retina occupied by Iba-1+ cells in the nerve fiber layer/ganglion cell layer from 1 d onward; v) increase in the number of vertical processes from 1 d in contralateral eyes and 3 d in OHT eyes. In OHT eyes at 24 h and 15 d, most Iba-1+ cells were P2RY12+ and were down-regulated at 3 and 5 d. In both eyes, microglial activation was stronger at 3 and 5 d (inflammation peaked in this model). These time points could be useful to identify factors implicated in the inflammatory process.

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