Determination of regioisomeric distribution in carbohydrate fatty acid monoesters by LC–ESI-MS

7 páginas, 5 figuras.

Detalhes bibliográficos
Autores: Pérez-Victoria, Ignacio, Zafra-Gómez, Alberto, Morales, Juan Carlos
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2007
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/36262
Acesso em linha:http://hdl.handle.net/10261/36262
Access Level:acceso abierto
Palavra-chave:Oligosaccharide
Oligosccharide fatty acid monoesters
Regioisomeric separation
Transesterification
Sucrose ester
LC-ESI-MS
id ES_d1fd85e573fb9cdca639b161ad2d3d6f
oai_identifier_str oai:digital.csic.es:10261/36262
network_acronym_str ES
network_name_str España
repository_id_str
spelling Determination of regioisomeric distribution in carbohydrate fatty acid monoesters by LC–ESI-MSPérez-Victoria, IgnacioZafra-Gómez, AlbertoMorales, Juan CarlosOligosaccharideOligosccharide fatty acid monoestersRegioisomeric separationTransesterificationSucrose esterLC-ESI-MS7 páginas, 5 figuras.A new LC–ESI-MS method for characterizing the regioisomeric distribution in carbohydrate monoesters with long-chain fatty acids is described. Sucrose monolaurate mixtures were used for development of the method. The surfactant nature and high polarity of these compounds make them appropriate analytes for being detected by electrospray-ionization mass spectrometry (ESI-MS). Despite the structural similarity of regioisomers, an excellent resolution of all regioisomers present in the different samples studied (sucrose monodecanoates, sucrose monolaurates, sucrose monopalmitates and melezitose monolaurates) is achieved. Reversed-phase liquid chromatography with isocratic acetonitrile–water mixtures was used for a proper separation of the analytes. Finally, the superiority of this chromatographic method for determining the regioselectivity in enzymatic carbohydrate acylation reactions, with respect to the typical methodology based on routine 13C NMR spectroscopy, is also discussed.Peer reviewedElsevierConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201120112007info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttp://hdl.handle.net/10261/36262reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.carres.2006.11.019Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/362622026-05-22T06:33:51Z
dc.title.none.fl_str_mv Determination of regioisomeric distribution in carbohydrate fatty acid monoesters by LC–ESI-MS
title Determination of regioisomeric distribution in carbohydrate fatty acid monoesters by LC–ESI-MS
spellingShingle Determination of regioisomeric distribution in carbohydrate fatty acid monoesters by LC–ESI-MS
Pérez-Victoria, Ignacio
Oligosaccharide
Oligosccharide fatty acid monoesters
Regioisomeric separation
Transesterification
Sucrose ester
LC-ESI-MS
title_short Determination of regioisomeric distribution in carbohydrate fatty acid monoesters by LC–ESI-MS
title_full Determination of regioisomeric distribution in carbohydrate fatty acid monoesters by LC–ESI-MS
title_fullStr Determination of regioisomeric distribution in carbohydrate fatty acid monoesters by LC–ESI-MS
title_full_unstemmed Determination of regioisomeric distribution in carbohydrate fatty acid monoesters by LC–ESI-MS
title_sort Determination of regioisomeric distribution in carbohydrate fatty acid monoesters by LC–ESI-MS
dc.creator.none.fl_str_mv Pérez-Victoria, Ignacio
Zafra-Gómez, Alberto
Morales, Juan Carlos
author Pérez-Victoria, Ignacio
author_facet Pérez-Victoria, Ignacio
Zafra-Gómez, Alberto
Morales, Juan Carlos
author_role author
author2 Zafra-Gómez, Alberto
Morales, Juan Carlos
author2_role author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Oligosaccharide
Oligosccharide fatty acid monoesters
Regioisomeric separation
Transesterification
Sucrose ester
LC-ESI-MS
topic Oligosaccharide
Oligosccharide fatty acid monoesters
Regioisomeric separation
Transesterification
Sucrose ester
LC-ESI-MS
description 7 páginas, 5 figuras.
publishDate 2007
dc.date.none.fl_str_mv 2007
2011
2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/36262
url http://hdl.handle.net/10261/36262
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.carres.2006.11.019
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869420311208787968
score 15,811543

Registos relacionados