Heat Stress and Betaine Affect Lipolysis in Pig Adipose Tissue Explants

Heat stress (HS) is an environmental variable challenging pig production worldwide. It may alter carcass composition by increasing fat deposition but the mechanisms are poorly understood. Betaine is a metabolic modifier able to decrease carcass fat in pigs, but the effects of betaine on lipolysis un...

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Autores: Pardo Domínguez, Zaira, Lachica, Manuel, Nieto, Rosa, Seiquer, Isabel, Fernández-Fígares, Ignacio
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.12327/4776
Acceso en línea:http://hdl.handle.net/20.500.12327/4776
https://doi.org/10.3390/ani15192845
Access Level:acceso abierto
Palabra clave:636
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spelling Heat Stress and Betaine Affect Lipolysis in Pig Adipose Tissue ExplantsPardo Domínguez, ZairaLachica, ManuelNieto, RosaSeiquer, IsabelFernández-Fígares, Ignacio636Heat stress (HS) is an environmental variable challenging pig production worldwide. It may alter carcass composition by increasing fat deposition but the mechanisms are poorly understood. Betaine is a metabolic modifier able to decrease carcass fat in pigs, but the effects of betaine on lipolysis under high temperatures are not known. Our goal was to determine a potential effect of betaine on lipolysis in the fat tissue of Iberian pigs under thermoneutral (TN) or HS conditions. Eight pigs (89 kg BW) were used to obtain explants from dorsal subcutaneous adipose tissue. Explants were cultivated under TN (37 ◦C) or HS conditions (41.5 ◦C) for 1.5 h to study direct effects of HS on acute lipolysis. Treatments included control and betaine addition (200 µM). Indirect effects of HS were examined using the culture medium amended with 1 µM isoproterenol ±10 nM insulin in the presence or absence of betaine and HS. Media glycerol was measured at the end of the incubations as a proxy of lipolysis. HS decreased basal lipolysis (−47%; p < 0.001) but increased isoproterenol-stimulated (a β-agonist) lipolysis (+31%; p < 0.01). Betaine increased non-stimulated lipolysis both under TN and HS conditions (73%; p < 0.001). As expected, isoproterenol augmented lipolysis both in TN (2714%; p < 0.001) and HS (3589%; p < 0.001) conditions. The addition of insulin to lipolysis-stimulated explants analogously diminished glycerol release in TN (−39%; p < 0.001) and HS (−50%; p < 0.001) conditions. In conclusion, HS reduced non-stimulated lipolysis in Iberian pigs in line with increased adipose tissue accretion, while betaine partly increased lipolysis, counteracting the effects of HS. Betaine supplementation could be a strategy to counteract HS-induced fattening in pigs.This research was funded by Ministerio de Economía y Competitividad, grant no. AGL 2016-80231. Z. Pardo received a scholarship FPI from Ministerio de Economía y Competitividad (reference BES-2017-081486) and was part of the PhD Thesis project within the Official Doctorate Program of Nutrition and Food Sciences (University of Granada, Spain).info:eu-repo/semantics/publishedVersionMDPIProducció AnimalNutrició Animal2025info:eu-repo/semantics/article12http://hdl.handle.net/20.500.12327/4776https://doi.org/10.3390/ani15192845reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésAnimalsMINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2016-80231-R/ES/Efectos fisiológicos y productivos del estrés por calor en el cerdo ibérico y posibles estrategias nutricionales para mitigarlo/Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:20.500.12327/47762026-05-29T05:05:01Z
dc.title.none.fl_str_mv Heat Stress and Betaine Affect Lipolysis in Pig Adipose Tissue Explants
title Heat Stress and Betaine Affect Lipolysis in Pig Adipose Tissue Explants
spellingShingle Heat Stress and Betaine Affect Lipolysis in Pig Adipose Tissue Explants
Pardo Domínguez, Zaira
636
title_short Heat Stress and Betaine Affect Lipolysis in Pig Adipose Tissue Explants
title_full Heat Stress and Betaine Affect Lipolysis in Pig Adipose Tissue Explants
title_fullStr Heat Stress and Betaine Affect Lipolysis in Pig Adipose Tissue Explants
title_full_unstemmed Heat Stress and Betaine Affect Lipolysis in Pig Adipose Tissue Explants
title_sort Heat Stress and Betaine Affect Lipolysis in Pig Adipose Tissue Explants
dc.creator.none.fl_str_mv Pardo Domínguez, Zaira
Lachica, Manuel
Nieto, Rosa
Seiquer, Isabel
Fernández-Fígares, Ignacio
author Pardo Domínguez, Zaira
author_facet Pardo Domínguez, Zaira
Lachica, Manuel
Nieto, Rosa
Seiquer, Isabel
Fernández-Fígares, Ignacio
author_role author
author2 Lachica, Manuel
Nieto, Rosa
Seiquer, Isabel
Fernández-Fígares, Ignacio
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Producció Animal
Nutrició Animal
dc.subject.none.fl_str_mv 636
topic 636
description Heat stress (HS) is an environmental variable challenging pig production worldwide. It may alter carcass composition by increasing fat deposition but the mechanisms are poorly understood. Betaine is a metabolic modifier able to decrease carcass fat in pigs, but the effects of betaine on lipolysis under high temperatures are not known. Our goal was to determine a potential effect of betaine on lipolysis in the fat tissue of Iberian pigs under thermoneutral (TN) or HS conditions. Eight pigs (89 kg BW) were used to obtain explants from dorsal subcutaneous adipose tissue. Explants were cultivated under TN (37 ◦C) or HS conditions (41.5 ◦C) for 1.5 h to study direct effects of HS on acute lipolysis. Treatments included control and betaine addition (200 µM). Indirect effects of HS were examined using the culture medium amended with 1 µM isoproterenol ±10 nM insulin in the presence or absence of betaine and HS. Media glycerol was measured at the end of the incubations as a proxy of lipolysis. HS decreased basal lipolysis (−47%; p < 0.001) but increased isoproterenol-stimulated (a β-agonist) lipolysis (+31%; p < 0.01). Betaine increased non-stimulated lipolysis both under TN and HS conditions (73%; p < 0.001). As expected, isoproterenol augmented lipolysis both in TN (2714%; p < 0.001) and HS (3589%; p < 0.001) conditions. The addition of insulin to lipolysis-stimulated explants analogously diminished glycerol release in TN (−39%; p < 0.001) and HS (−50%; p < 0.001) conditions. In conclusion, HS reduced non-stimulated lipolysis in Iberian pigs in line with increased adipose tissue accretion, while betaine partly increased lipolysis, counteracting the effects of HS. Betaine supplementation could be a strategy to counteract HS-induced fattening in pigs.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12327/4776
https://doi.org/10.3390/ani15192845
url http://hdl.handle.net/20.500.12327/4776
https://doi.org/10.3390/ani15192845
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Animals
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2016-80231-R/ES/Efectos fisiológicos y productivos del estrés por calor en el cerdo ibérico y posibles estrategias nutricionales para mitigarlo/
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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