Developmental guidance of the retroflex tract at its bending point involves Robo1-Slit2-mediated floor plate repulsion

The retroflex tract contains medial habenula efferents that target the hindbrain interpeduncular complex and surrounding areas. This tract displays a singular course. Initially, habenular axons extend ventralwards in front of the pretectum until they reach the basal plate. Next, they avoid crossing...

Descripción completa

Detalles Bibliográficos
Autores: Moreno-Bravo, Juan Antonio, Martínez-López, Jesús E., Madrigal Verdú, Pilar, Kim, Minkyung, Mastick, Grant S., López-Bendito, Guillermina, Martinez, Salvador, Puelles, Eduardo
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Universidad Miguel Hernández de Elche
Repositorio:REDIUMH. Depósito Digital de la UMH
OAI Identifier:oai:dspace.umh.es:11000/35382
Acceso en línea:https://hdl.handle.net/11000/35382
Access Level:acceso abierto
Palabra clave:Habenula
retroflex tract
Robo-Slit
axon guidance
id ES_d1ebdf1fa876e73727482f033f13f987
oai_identifier_str oai:dspace.umh.es:11000/35382
network_acronym_str ES
network_name_str España
repository_id_str
spelling Developmental guidance of the retroflex tract at its bending point involves Robo1-Slit2-mediated floor plate repulsionMoreno-Bravo, Juan AntonioMartínez-López, Jesús E.Madrigal Verdú, PilarKim, MinkyungMastick, Grant S.López-Bendito, GuillerminaMartinez, SalvadorPuelles, EduardoHabenularetroflex tractRobo-Slitaxon guidanceThe retroflex tract contains medial habenula efferents that target the hindbrain interpeduncular complex and surrounding areas. This tract displays a singular course. Initially, habenular axons extend ventralwards in front of the pretectum until they reach the basal plate. Next, they avoid crossing the local floor plate, sharply changing course caudalwards (the retroflexion alluded by the tract name) and navigate strictly antero-posteriorly across basal pretectum, midbrain and isthmus. Once they reach rhombomere 1, the habenular axons criss-cross the floor plate several times within the interpeduncular nuclear complex as they innervate it. Here we described the timing and details of growth phenomena as these axons navigate to their target. The first dorsoventral course apparently obeys Ntn1 attraction. We checked the role of local floor plate signaling in the decision to avoid the thalamic floor plate and bend caudalwards. Analyzing the altered floor and basal plates of Gli2 knockout mice, we found a contralateral projection of most habenular axons, plus ulterior bizarre navigation rostralwards. This crossing phenotype was due to a reduced expression of Slit repulsive cues, suggesting involvement of the floor-derived Robo-Slit system in the normal guidance of this tract. Using Slit and Robo mutant mice, open neural tube and co-culture assays, we determined that Robo1-Slit2 interaction is specifically required for impeding that medial habenular axons cross the thalamic floor plate. This pathfinding mechanism is essential to establish the functionally important habenulo-interpeduncular connection.Springer NatureDepartamentos de la UMH::Histología y AnatomíaInstitutos de la UMH::Instituto de Neurociencias202520252016info:eu-repo/semantics/articleapplication/pdf20application/pdfhttps://hdl.handle.net/11000/35382reponame:REDIUMH. Depósito Digital de la UMHinstname:Universidad Miguel Hernández de ElcheInglés10.1007/s00429-014-0932-4info:eu-repo/semantics/openAccessAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/oai:dspace.umh.es:11000/353822026-05-27T13:36:21Z
dc.title.none.fl_str_mv Developmental guidance of the retroflex tract at its bending point involves Robo1-Slit2-mediated floor plate repulsion
title Developmental guidance of the retroflex tract at its bending point involves Robo1-Slit2-mediated floor plate repulsion
spellingShingle Developmental guidance of the retroflex tract at its bending point involves Robo1-Slit2-mediated floor plate repulsion
Moreno-Bravo, Juan Antonio
Habenula
retroflex tract
Robo-Slit
axon guidance
title_short Developmental guidance of the retroflex tract at its bending point involves Robo1-Slit2-mediated floor plate repulsion
title_full Developmental guidance of the retroflex tract at its bending point involves Robo1-Slit2-mediated floor plate repulsion
title_fullStr Developmental guidance of the retroflex tract at its bending point involves Robo1-Slit2-mediated floor plate repulsion
title_full_unstemmed Developmental guidance of the retroflex tract at its bending point involves Robo1-Slit2-mediated floor plate repulsion
title_sort Developmental guidance of the retroflex tract at its bending point involves Robo1-Slit2-mediated floor plate repulsion
dc.creator.none.fl_str_mv Moreno-Bravo, Juan Antonio
Martínez-López, Jesús E.
Madrigal Verdú, Pilar
Kim, Minkyung
Mastick, Grant S.
López-Bendito, Guillermina
Martinez, Salvador
Puelles, Eduardo
author Moreno-Bravo, Juan Antonio
author_facet Moreno-Bravo, Juan Antonio
Martínez-López, Jesús E.
Madrigal Verdú, Pilar
Kim, Minkyung
Mastick, Grant S.
López-Bendito, Guillermina
Martinez, Salvador
Puelles, Eduardo
author_role author
author2 Martínez-López, Jesús E.
Madrigal Verdú, Pilar
Kim, Minkyung
Mastick, Grant S.
López-Bendito, Guillermina
Martinez, Salvador
Puelles, Eduardo
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamentos de la UMH::Histología y Anatomía
Institutos de la UMH::Instituto de Neurociencias
dc.subject.none.fl_str_mv Habenula
retroflex tract
Robo-Slit
axon guidance
topic Habenula
retroflex tract
Robo-Slit
axon guidance
description The retroflex tract contains medial habenula efferents that target the hindbrain interpeduncular complex and surrounding areas. This tract displays a singular course. Initially, habenular axons extend ventralwards in front of the pretectum until they reach the basal plate. Next, they avoid crossing the local floor plate, sharply changing course caudalwards (the retroflexion alluded by the tract name) and navigate strictly antero-posteriorly across basal pretectum, midbrain and isthmus. Once they reach rhombomere 1, the habenular axons criss-cross the floor plate several times within the interpeduncular nuclear complex as they innervate it. Here we described the timing and details of growth phenomena as these axons navigate to their target. The first dorsoventral course apparently obeys Ntn1 attraction. We checked the role of local floor plate signaling in the decision to avoid the thalamic floor plate and bend caudalwards. Analyzing the altered floor and basal plates of Gli2 knockout mice, we found a contralateral projection of most habenular axons, plus ulterior bizarre navigation rostralwards. This crossing phenotype was due to a reduced expression of Slit repulsive cues, suggesting involvement of the floor-derived Robo-Slit system in the normal guidance of this tract. Using Slit and Robo mutant mice, open neural tube and co-culture assays, we determined that Robo1-Slit2 interaction is specifically required for impeding that medial habenular axons cross the thalamic floor plate. This pathfinding mechanism is essential to establish the functionally important habenulo-interpeduncular connection.
publishDate 2016
dc.date.none.fl_str_mv 2016
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/11000/35382
url https://hdl.handle.net/11000/35382
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv 10.1007/s00429-014-0932-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.none.fl_str_mv application/pdf
20
application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:REDIUMH. Depósito Digital de la UMH
instname:Universidad Miguel Hernández de Elche
instname_str Universidad Miguel Hernández de Elche
reponame_str REDIUMH. Depósito Digital de la UMH
collection REDIUMH. Depósito Digital de la UMH
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869420305117609984
score 15,811543

Similares en LA Referencia