Independent roles of Drosophila Moesin in imaginal disc morphogenesis and hedgehog signalling

The three ERM proteins (Ezrin, Radixin and Moesin) form a conserved family required in many developmental processes involving regulation of the cytoskeleton. In general, the molecular function of ERM proteins is to link specific membrane proteins to the actin cytoskeleton. In Drosophila, loss of moe...

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Detalles Bibliográficos
Autores: Molnar, Cristina, Celis, José F. de
Tipo de recurso: artículo
Fecha de publicación:2006
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/4734
Acceso en línea:http://hdl.handle.net/10261/4734
Access Level:acceso abierto
Palabra clave:Moesin
Hedgehog signalling
Epithelial morphogenesis
Pattern formation
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spelling Independent roles of Drosophila Moesin in imaginal disc morphogenesis and hedgehog signallingMolnar, CristinaCelis, José F. deMoesinHedgehog signallingEpithelial morphogenesisPattern formationThe three ERM proteins (Ezrin, Radixin and Moesin) form a conserved family required in many developmental processes involving regulation of the cytoskeleton. In general, the molecular function of ERM proteins is to link specific membrane proteins to the actin cytoskeleton. In Drosophila, loss of moesin (moe) activity causes incorrect localisation of maternal determinants during oogenesis, failures in rhabdomere differentiation in the eye and alterations of epithelial integrity in the wing imaginal disc. Some aspects of Drosophila Moe are related to the activity of the small GTPase RhoA, because the reduction of RhoA activity corrects many phenotypes of moe mutant embryos and imaginal discs. We have analysed the phenotype of moesin loss-of-function alleles in the wing disc and adult wing, and studied the effects of reduced Moesin activity on signalling mediated by the Notch, Decapentaplegic, Wingless and Hedgehog pathways. We found that reductions in Moesin levels in the wing disc cause the formation of wing-tissue vesicles and large thickenings of the vein L3, corresponding to breakdowns of epithelial continuity in the wing base and modifications of Hedgehog signalling in the wing blade, respectively. We did not observe any effect on signalling pathways other than Hedgehog, indicating that the moe defects in epithelial integrity have not generalised effects on cell signalling. The effects of moe mutants on Hedgehog signalling depend on the correct gene-dose of rhoA, suggesting that the requirements for Moesin in disc morphogenesis and Hh signalling in the wing disc are mediated by its regulation of RhoA activity. The mechanism linking Moesin activity with RhoA function and Hedgehog signalling remains to be elucidatedGrants from Dirección General de Investigación Científica y Técnica (BCM2003-1191 and GEN2001-4846-C05-01) to J.F.d C. and an institutional grant from Fundación Ramón Areces to the Centro de Biología Molecular “Severo Ochoa” are also acknowledged.Peer reviewedElsevierMinisterio de Economía y Competitividad (España)Fundación Ramón Areces200820082006info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_65012486270 bytesapplication/pdfhttp://hdl.handle.net/10261/4734reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.mod.2006.02.001info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/47342026-05-22T06:33:51Z
dc.title.none.fl_str_mv Independent roles of Drosophila Moesin in imaginal disc morphogenesis and hedgehog signalling
title Independent roles of Drosophila Moesin in imaginal disc morphogenesis and hedgehog signalling
spellingShingle Independent roles of Drosophila Moesin in imaginal disc morphogenesis and hedgehog signalling
Molnar, Cristina
Moesin
Hedgehog signalling
Epithelial morphogenesis
Pattern formation
title_short Independent roles of Drosophila Moesin in imaginal disc morphogenesis and hedgehog signalling
title_full Independent roles of Drosophila Moesin in imaginal disc morphogenesis and hedgehog signalling
title_fullStr Independent roles of Drosophila Moesin in imaginal disc morphogenesis and hedgehog signalling
title_full_unstemmed Independent roles of Drosophila Moesin in imaginal disc morphogenesis and hedgehog signalling
title_sort Independent roles of Drosophila Moesin in imaginal disc morphogenesis and hedgehog signalling
dc.creator.none.fl_str_mv Molnar, Cristina
Celis, José F. de
author Molnar, Cristina
author_facet Molnar, Cristina
Celis, José F. de
author_role author
author2 Celis, José F. de
author2_role author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Fundación Ramón Areces
dc.subject.none.fl_str_mv Moesin
Hedgehog signalling
Epithelial morphogenesis
Pattern formation
topic Moesin
Hedgehog signalling
Epithelial morphogenesis
Pattern formation
description The three ERM proteins (Ezrin, Radixin and Moesin) form a conserved family required in many developmental processes involving regulation of the cytoskeleton. In general, the molecular function of ERM proteins is to link specific membrane proteins to the actin cytoskeleton. In Drosophila, loss of moesin (moe) activity causes incorrect localisation of maternal determinants during oogenesis, failures in rhabdomere differentiation in the eye and alterations of epithelial integrity in the wing imaginal disc. Some aspects of Drosophila Moe are related to the activity of the small GTPase RhoA, because the reduction of RhoA activity corrects many phenotypes of moe mutant embryos and imaginal discs. We have analysed the phenotype of moesin loss-of-function alleles in the wing disc and adult wing, and studied the effects of reduced Moesin activity on signalling mediated by the Notch, Decapentaplegic, Wingless and Hedgehog pathways. We found that reductions in Moesin levels in the wing disc cause the formation of wing-tissue vesicles and large thickenings of the vein L3, corresponding to breakdowns of epithelial continuity in the wing base and modifications of Hedgehog signalling in the wing blade, respectively. We did not observe any effect on signalling pathways other than Hedgehog, indicating that the moe defects in epithelial integrity have not generalised effects on cell signalling. The effects of moe mutants on Hedgehog signalling depend on the correct gene-dose of rhoA, suggesting that the requirements for Moesin in disc morphogenesis and Hh signalling in the wing disc are mediated by its regulation of RhoA activity. The mechanism linking Moesin activity with RhoA function and Hedgehog signalling remains to be elucidated
publishDate 2006
dc.date.none.fl_str_mv 2006
2008
2008
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/4734
url http://hdl.handle.net/10261/4734
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.mod.2006.02.001
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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