Overexpression of Scr1 protein induces strong changes in the transcriptome and metabolome of Streptomyces coelicolor that affect antibiotic production

Xenobiotic response element (XRE) regulator proteins play an important role in gene regulation in Streptomyces. In these bacteria, they are often encoded together with a small protein containing a DUF397 domain. Previous work on 15 such pairs present in S. coelicolor has shown they act as pleiotropi...

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Detalhes bibliográficos
Autores: Riascos, Carolina, Fernández-García, Gemma, García Martín, Javier, Manteca, Ángel, Santamaría, Ramón I., Díaz, Margarita
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2025
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/418637
Acesso em linha:http://hdl.handle.net/10261/418637
https://api.elsevier.com/content/abstract/scopus_id/105018527981
Access Level:Acceso aberto
Palavra-chave:Gene regulation
Metabolome
Streptomyces
Transcriptome
XRE/DUF397
Descrição
Resumo:Xenobiotic response element (XRE) regulator proteins play an important role in gene regulation in Streptomyces. In these bacteria, they are often encoded together with a small protein containing a DUF397 domain. Previous work on 15 such pairs present in S. coelicolor has shown they act as pleiotropic regulators. They dependently affect antibiotic production and morphological differentiation on different culture media. Overexpression of the protein Scr1, which is encoded by gene SCO4441, induces a drastic increase in the production of the antibiotic actinorhodin in this bacterium. This study involves a transcriptomic and metabolomic study of this overexpression.